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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Legionella pneumophila GTPase obg (obg) CSB-YP726211LAAA
CSB-EP726211LAAA
CSB-BP726211LAAA
CSB-MP726211LAAA
CSB-EP726211LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP726212LAAA
CSB-EP726212LAAA
CSB-BP726212LAAA
CSB-MP726212LAAA
CSB-EP726212LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Non-canonical purine NTP pyrophosphatase (lpl2404) CSB-YP726213LAAA
CSB-EP726213LAAA
CSB-BP726213LAAA
CSB-MP726213LAAA
CSB-EP726213LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP726214LAAA1
CSB-EP726214LAAA1
CSB-BP726214LAAA1
CSB-MP726214LAAA1
CSB-EP726214LAAA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Maf-like protein lpl2014 (lpl2014) CSB-YP726215LAAA
CSB-EP726215LAAA
CSB-BP726215LAAA
CSB-MP726215LAAA
CSB-EP726215LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Chaperone protein DnaK (dnaK), partial CSB-YP726216LAAA
CSB-EP726216LAAA
CSB-BP726216LAAA
CSB-MP726216LAAA
CSB-EP726216LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP726217LAAA
CSB-EP726217LAAA
CSB-BP726217LAAA
CSB-MP726217LAAA
CSB-EP726217LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP726218LAAA
CSB-EP726218LAAA
CSB-BP726218LAAA
CSB-MP726218LAAA
CSB-EP726218LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Trigger factor (tig) CSB-YP726219LAAA
CSB-EP726219LAAA
CSB-BP726219LAAA
CSB-MP726219LAAA
CSB-EP726219LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP726220LAAA
CSB-EP726220LAAA
CSB-BP726220LAAA
CSB-MP726220LAAA
CSB-EP726220LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B (gatB) CSB-YP726221LAAA
CSB-EP726221LAAA
CSB-BP726221LAAA
CSB-MP726221LAAA
CSB-EP726221LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Protein translocase subunit SecA (secA), partial CSB-YP726222LAAA
CSB-EP726222LAAA
CSB-BP726222LAAA
CSB-MP726222LAAA
CSB-EP726222LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Octanoyltransferase (lipB) CSB-YP726223LAAA
CSB-EP726223LAAA
CSB-BP726223LAAA
CSB-MP726223LAAA
CSB-EP726223LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Histidine--tRNA ligase (hisS) CSB-YP726224LAAA
CSB-EP726224LAAA
CSB-BP726224LAAA
CSB-MP726224LAAA
CSB-EP726224LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP726225LAAA
CSB-EP726225LAAA
CSB-BP726225LAAA
CSB-MP726225LAAA
CSB-EP726225LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Ribonuclease HII (rnhB) CSB-YP726226LAAA
CSB-EP726226LAAA
CSB-BP726226LAAA
CSB-MP726226LAAA
CSB-EP726226LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Cysteine--tRNA ligase (cysS) CSB-YP726227LAAA
CSB-EP726227LAAA
CSB-BP726227LAAA
CSB-MP726227LAAA
CSB-EP726227LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Probable transcriptional regulatory protein lpl1249 (lpl1249) CSB-YP726228LAAA
CSB-EP726228LAAA
CSB-BP726228LAAA
CSB-MP726228LAAA
CSB-EP726228LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Probable potassium transport system protein kup 1 (kup1), partial CSB-YP726229LAAA
CSB-EP726229LAAA
CSB-BP726229LAAA
CSB-MP726229LAAA
CSB-EP726229LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila DNA ligase (ligA), partial CSB-YP726230LAAA
CSB-EP726230LAAA
CSB-BP726230LAAA
CSB-MP726230LAAA
CSB-EP726230LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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