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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Debaryomyces hansenii DASH complex subunit SPC19 (SPC19) CSB-YP728163DIS
CSB-EP728163DIS
CSB-BP728163DIS
CSB-MP728163DIS
CSB-EP728163DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Enolase 2 (ENO2) CSB-YP728164DIS
CSB-EP728164DIS
CSB-BP728164DIS
CSB-MP728164DIS
CSB-EP728164DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Putative heme-binding peroxidase (DEHA2G12166g) CSB-YP728165DIS
CSB-EP728165DIS
CSB-BP728165DIS
CSB-MP728165DIS
CSB-EP728165DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Mediator of RNA polymerase II transcription subunit 6 (MED6) CSB-YP728166DIS
CSB-EP728166DIS
CSB-BP728166DIS
CSB-MP728166DIS
CSB-EP728166DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii DNA polymerase epsilon subunit D (DPB4) CSB-YP728167DIS
CSB-EP728167DIS
CSB-BP728167DIS
CSB-MP728167DIS
CSB-EP728167DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Protein TOS6 (TOS6) CSB-YP728168DIS
CSB-EP728168DIS
CSB-BP728168DIS
CSB-MP728168DIS
CSB-EP728168DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Elongation factor 2 (EFT1), partial CSB-YP728169DIS
CSB-EP728169DIS
CSB-BP728169DIS
CSB-MP728169DIS
CSB-EP728169DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Sensitive to high expression protein 9 homolog, mitochondrial (SHE9), partial CSB-YP728170DIS
CSB-EP728170DIS
CSB-BP728170DIS
CSB-MP728170DIS
CSB-EP728170DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii N-acetyltransferase ECO1 (ECO1) CSB-YP728171DIS
CSB-EP728171DIS
CSB-BP728171DIS
CSB-MP728171DIS
CSB-EP728171DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Catabolic 3-dehydroquinase (DQD1) CSB-YP728172DIS
CSB-EP728172DIS
CSB-BP728172DIS
CSB-MP728172DIS
CSB-EP728172DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii WD repeat-containing protein DEHA2F21868g (DEHA2F21868g), partial CSB-YP728173DIS
CSB-EP728173DIS
CSB-BP728173DIS
CSB-MP728173DIS
CSB-EP728173DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Pro-apoptotic serine protease NMA111 (NMA111), partial CSB-YP728174DIS
CSB-EP728174DIS
CSB-BP728174DIS
CSB-MP728174DIS
CSB-EP728174DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Copper transport protein 86 (CTR86) CSB-YP728175DIS
CSB-EP728175DIS
CSB-BP728175DIS
CSB-MP728175DIS
CSB-EP728175DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Cytochrome c peroxidase, mitochondrial (CCP1), partial CSB-YP728176DIS
CSB-EP728176DIS
CSB-BP728176DIS
CSB-MP728176DIS
CSB-EP728176DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Phosphoglycerate kinase (PGK1) CSB-YP728177DIS
CSB-EP728177DIS
CSB-BP728177DIS
CSB-MP728177DIS
CSB-EP728177DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Serine/threonine-protein kinase CBK1 (CBK1), partial CSB-YP728178DIS
CSB-EP728178DIS
CSB-BP728178DIS
CSB-MP728178DIS
CSB-EP728178DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Ribosome biogenesis protein YTM1 (YTM1) CSB-YP728179DIS
CSB-EP728179DIS
CSB-BP728179DIS
CSB-MP728179DIS
CSB-EP728179DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Nascent polypeptide-associated complex subunit beta (EGD1) CSB-YP728180DIS
CSB-EP728180DIS
CSB-BP728180DIS
CSB-MP728180DIS
CSB-EP728180DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Peroxisomal targeting signal receptor (PEX5), partial CSB-YP728181DIS
CSB-EP728181DIS
CSB-BP728181DIS
CSB-MP728181DIS
CSB-EP728181DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Signal recognition particle SEC65 subunit (SEC65) CSB-YP728182DIS
CSB-EP728182DIS
CSB-BP728182DIS
CSB-MP728182DIS
CSB-EP728182DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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