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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mouse Protein yippee-like 4 (Ypel4) CSB-YP727582MO
CSB-EP727582MO
CSB-BP727582MO
CSB-MP727582MO
CSB-EP727582MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Isoleucine--tRNA ligase (ileS), partial CSB-YP727583BUI
CSB-EP727583BUI
CSB-BP727583BUI
CSB-MP727583BUI
CSB-EP727583BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosome-binding factor A (rbfA) CSB-YP727584BUI
CSB-EP727584BUI
CSB-BP727584BUI
CSB-MP727584BUI
CSB-EP727584BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative septation protein spoVG (spoVG) CSB-YP727585BUI
CSB-EP727585BUI
CSB-BP727585BUI
CSB-MP727585BUI
CSB-EP727585BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Canine distemper virus Hemagglutinin glycoprotein (H), partial CSB-YP727586CGAU1
CSB-EP727586CGAU1
CSB-BP727586CGAU1
CSB-MP727586CGAU1
CSB-EP727586CGAU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L32 (rpmF) CSB-YP727587BUI
CSB-EP727587BUI
CSB-BP727587BUI
CSB-MP727587BUI
CSB-EP727587BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor G 2 (fusA2), partial CSB-YP727588BUI
CSB-EP727588BUI
CSB-BP727588BUI
CSB-MP727588BUI
CSB-EP727588BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP727589BUI
CSB-EP727589BUI
CSB-BP727589BUI
CSB-MP727589BUI
CSB-EP727589BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spermidine/putrescine import ATP-binding protein PotA (potA) CSB-YP727590BUI
CSB-EP727590BUI
CSB-BP727590BUI
CSB-MP727590BUI
CSB-EP727590BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Type III pantothenate kinase (coaX) CSB-YP727591BUI
CSB-EP727591BUI
CSB-BP727591BUI
CSB-MP727591BUI
CSB-EP727591BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucumber mosaic virus Movement protein (ORF3a) CSB-YP727592CFAU
CSB-EP727592CFAU
CSB-BP727592CFAU
CSB-MP727592CFAU
CSB-EP727592CFAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucumber mosaic virus Movement protein (ORF3a) CSB-YP727593CFAV
CSB-EP727593CFAV
CSB-BP727593CFAV
CSB-MP727593CFAV
CSB-EP727593CFAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S11 (rpsK) CSB-YP727594BUI
CSB-EP727594BUI
CSB-BP727594BUI
CSB-MP727594BUI
CSB-EP727594BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L14 (rplN) CSB-YP727595BUI
CSB-EP727595BUI
CSB-BP727595BUI
CSB-MP727595BUI
CSB-EP727595BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S10 (rpsJ) CSB-YP727596BUI
CSB-EP727596BUI
CSB-BP727596BUI
CSB-MP727596BUI
CSB-EP727596BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UvrABC system protein C (uvrC), partial CSB-YP727597BUI
CSB-EP727597BUI
CSB-BP727597BUI
CSB-MP727597BUI
CSB-EP727597BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Membrane protein insertase YidC (yidC), partial CSB-YP727598BUI1
CSB-EP727598BUI1
CSB-BP727598BUI1
CSB-MP727598BUI1
CSB-EP727598BUI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP727599BUI
CSB-EP727599BUI
CSB-BP727599BUI
CSB-MP727599BUI
CSB-EP727599BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable M18 family aminopeptidase 1 (apeA) CSB-YP727600BUI
CSB-EP727600BUI
CSB-BP727600BUI
CSB-MP727600BUI
CSB-EP727600BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamyl-tRNA (Gln) amidotransferase subunit A (gatA) CSB-YP727601BUI
CSB-EP727601BUI
CSB-BP727601BUI
CSB-MP727601BUI
CSB-EP727601BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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