Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus licheniformis UPF0477 protein BLi01284/BL02661 (BLi01284, BL02661) CSB-YP727502BQU
CSB-EP727502BQU
CSB-BP727502BQU
CSB-MP727502BQU
CSB-EP727502BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis GMP reductase (guaC) CSB-YP727503BQU
CSB-EP727503BQU
CSB-BP727503BQU
CSB-MP727503BQU
CSB-EP727503BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis 30S ribosomal protein S14 (rpsN) CSB-YP727504BQU
CSB-EP727504BQU
CSB-BP727504BQU
CSB-MP727504BQU
CSB-EP727504BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis Acylphosphatase (acyP) CSB-YP727505BQU
CSB-EP727505BQU
CSB-BP727505BQU
CSB-MP727505BQU
CSB-EP727505BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B (gatB) CSB-YP727506BQU
CSB-EP727506BQU
CSB-BP727506BQU
CSB-MP727506BQU
CSB-EP727506BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP727507BQU
CSB-EP727507BQU
CSB-BP727507BQU
CSB-MP727507BQU
CSB-EP727507BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP727508BQU
CSB-EP727508BQU
CSB-BP727508BQU
CSB-MP727508BQU
CSB-EP727508BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis DEAD-box ATP-dependent RNA helicase CshA (cshA) CSB-YP727509BQU
CSB-EP727509BQU
CSB-BP727509BQU
CSB-MP727509BQU
CSB-EP727509BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis Spore germination lipase lipC (lipC) CSB-YP727510BQU
CSB-EP727510BQU
CSB-BP727510BQU
CSB-MP727510BQU
CSB-EP727510BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS), partial CSB-YP727511BQU
CSB-EP727511BQU
CSB-BP727511BQU
CSB-MP727511BQU
CSB-EP727511BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis tRNA pseudouridine synthase A (truA) CSB-YP727512BQU
CSB-EP727512BQU
CSB-BP727512BQU
CSB-MP727512BQU
CSB-EP727512BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis 50S ribosomal protein L6 (rplF) CSB-YP727513BQU
CSB-EP727513BQU
CSB-BP727513BQU
CSB-MP727513BQU
CSB-EP727513BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis 30S ribosomal protein S19 (rpsS) CSB-YP727514BQU
CSB-EP727514BQU
CSB-BP727514BQU
CSB-MP727514BQU
CSB-EP727514BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis 50S ribosomal protein L3 (rplC) CSB-YP727515BQU
CSB-EP727515BQU
CSB-BP727515BQU
CSB-MP727515BQU
CSB-EP727515BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP727516BQU
CSB-EP727516BQU
CSB-BP727516BQU
CSB-MP727516BQU
CSB-EP727516BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis 50S ribosomal protein L11 (rplK) CSB-YP727517BQU
CSB-EP727517BQU
CSB-BP727517BQU
CSB-MP727517BQU
CSB-EP727517BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP727518BQU
CSB-EP727518BQU
CSB-BP727518BQU
CSB-MP727518BQU
CSB-EP727518BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis tRNA (Ile)-lysidine synthase (tilS) CSB-YP727519BQU
CSB-EP727519BQU
CSB-BP727519BQU
CSB-MP727519BQU
CSB-EP727519BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP727520BQU
CSB-EP727520BQU
CSB-BP727520BQU
CSB-MP727520BQU
CSB-EP727520BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis Initiation-control protein yabA (BLi00046, BL00538) CSB-YP727521BQU
CSB-EP727521BQU
CSB-BP727521BQU
CSB-MP727521BQU
CSB-EP727521BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>