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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant UPF0145 protein STH1265 (STH1265) CSB-YP727822SRW
CSB-EP727822SRW
CSB-BP727822SRW
CSB-MP727822SRW
CSB-EP727822SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP727823SRW
CSB-EP727823SRW
CSB-BP727823SRW
CSB-MP727823SRW
CSB-EP727823SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP727824SRW
CSB-EP727824SRW
CSB-BP727824SRW
CSB-MP727824SRW
CSB-EP727824SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L20 (rplT) CSB-YP727825SRW
CSB-EP727825SRW
CSB-BP727825SRW
CSB-MP727825SRW
CSB-EP727825SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sugar fermentation stimulation protein homolog (sfsA) CSB-YP727826SRW
CSB-EP727826SRW
CSB-BP727826SRW
CSB-MP727826SRW
CSB-EP727826SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphate import ATP-binding protein PstB 2 (pstB2) CSB-YP727827SRW
CSB-EP727827SRW
CSB-BP727827SRW
CSB-MP727827SRW
CSB-EP727827SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable rRNA maturation factor (STH531) CSB-YP727828SRW
CSB-EP727828SRW
CSB-BP727828SRW
CSB-MP727828SRW
CSB-EP727828SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L27 (rpmA) CSB-YP727829SRW
CSB-EP727829SRW
CSB-BP727829SRW
CSB-MP727829SRW
CSB-EP727829SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Valine--tRNA ligase (valS), partial CSB-YP727830SRW
CSB-EP727830SRW
CSB-BP727830SRW
CSB-MP727830SRW
CSB-EP727830SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant SsrA-binding protein (smpB) CSB-YP727831SRW
CSB-EP727831SRW
CSB-BP727831SRW
CSB-MP727831SRW
CSB-EP727831SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative sporulation transcription regulator WhiA (whiA) CSB-YP727832SRW
CSB-EP727832SRW
CSB-BP727832SRW
CSB-MP727832SRW
CSB-EP727832SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flagellar assembly factor FliW (fliW) CSB-YP727833SRW
CSB-EP727833SRW
CSB-BP727833SRW
CSB-MP727833SRW
CSB-EP727833SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit delta (atpH) CSB-YP727834SRW
CSB-EP727834SRW
CSB-BP727834SRW
CSB-MP727834SRW
CSB-EP727834SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L31 (rpmE) CSB-YP727835SRW
CSB-EP727835SRW
CSB-BP727835SRW
CSB-MP727835SRW
CSB-EP727835SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromosomal replication initiator protein DnaA (dnaA) CSB-YP727836SRW
CSB-EP727836SRW
CSB-BP727836SRW
CSB-MP727836SRW
CSB-EP727836SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable auxin efflux carrier component 1c (PIN1C), partial CSB-YP727837OFG
CSB-EP727837OFG
CSB-BP727837OFG
CSB-MP727837OFG
CSB-EP727837OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica tRNA (guanine-N (7)-)-methyltransferase (Os06g0236900, LOC_Os06g12990) CSB-YP727838OFG
CSB-EP727838OFG
CSB-BP727838OFG
CSB-MP727838OFG
CSB-EP727838OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable 4-coumarate--CoA ligase 4 (4CL4), partial CSB-YP727839OFG
CSB-EP727839OFG
CSB-BP727839OFG
CSB-MP727839OFG
CSB-EP727839OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Casparian strip membrane protein Os06g0231050 (Os06g0231050, LOC_Os06g12500), partial CSB-YP727840OFG1
CSB-EP727840OFG1
CSB-BP727840OFG1
CSB-MP727840OFG1
CSB-EP727840OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box protein At5g06550 (At5g06550), partial CSB-YP727841DOA
CSB-EP727841DOA
CSB-BP727841DOA
CSB-MP727841DOA
CSB-EP727841DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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