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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Idiomarina loihiensis Probable multidrug resistance protein norM (norM), partial CSB-YP728836IAAA
CSB-EP728836IAAA
CSB-BP728836IAAA
CSB-MP728836IAAA
CSB-EP728836IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis Argininosuccinate synthase (argG) CSB-YP728837IAAA
CSB-EP728837IAAA
CSB-BP728837IAAA
CSB-MP728837IAAA
CSB-EP728837IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis Glutamate--tRNA ligase (gltX) CSB-YP728838IAAA
CSB-EP728838IAAA
CSB-BP728838IAAA
CSB-MP728838IAAA
CSB-EP728838IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis 3-ketoacyl-CoA thiolase (fadA) CSB-YP728839IAAA
CSB-EP728839IAAA
CSB-BP728839IAAA
CSB-MP728839IAAA
CSB-EP728839IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis Dihydrodipicolinate reductase (dapB) CSB-YP728840IAAA
CSB-EP728840IAAA
CSB-BP728840IAAA
CSB-MP728840IAAA
CSB-EP728840IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP728841IAAA
CSB-EP728841IAAA
CSB-BP728841IAAA
CSB-MP728841IAAA
CSB-EP728841IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP728842IAAA
CSB-EP728842IAAA
CSB-BP728842IAAA
CSB-MP728842IAAA
CSB-EP728842IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP728843IAAA
CSB-EP728843IAAA
CSB-BP728843IAAA
CSB-MP728843IAAA
CSB-EP728843IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis 30S ribosomal protein S13 (rpsM) CSB-YP728844IAAA
CSB-EP728844IAAA
CSB-BP728844IAAA
CSB-MP728844IAAA
CSB-EP728844IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP728845IAAA
CSB-EP728845IAAA
CSB-BP728845IAAA
CSB-MP728845IAAA
CSB-EP728845IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis 30S ribosomal protein S17 (rpsQ) CSB-YP728846IAAA
CSB-EP728846IAAA
CSB-BP728846IAAA
CSB-MP728846IAAA
CSB-EP728846IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis 50S ribosomal protein L16 (rplP) CSB-YP728847IAAA
CSB-EP728847IAAA
CSB-BP728847IAAA
CSB-MP728847IAAA
CSB-EP728847IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis Regulator of ribonuclease activity B (rraB) CSB-YP728848IAAA
CSB-EP728848IAAA
CSB-BP728848IAAA
CSB-MP728848IAAA
CSB-EP728848IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP728849IAAA
CSB-EP728849IAAA
CSB-BP728849IAAA
CSB-MP728849IAAA
CSB-EP728849IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP728850IAAA
CSB-EP728850IAAA
CSB-BP728850IAAA
CSB-MP728850IAAA
CSB-EP728850IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP728851IAAA
CSB-EP728851IAAA
CSB-BP728851IAAA
CSB-MP728851IAAA
CSB-EP728851IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis ATP-dependent RNA helicase rhlB (rhlB) CSB-YP728852IAAA
CSB-EP728852IAAA
CSB-BP728852IAAA
CSB-MP728852IAAA
CSB-EP728852IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis 50S ribosomal protein L35 (rpmI) CSB-YP728853IAAA
CSB-EP728853IAAA
CSB-BP728853IAAA
CSB-MP728853IAAA
CSB-EP728853IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP728854IAAA
CSB-EP728854IAAA
CSB-BP728854IAAA
CSB-MP728854IAAA
CSB-EP728854IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis Lipid-A-disaccharide synthase (lpxB) CSB-YP728855IAAA
CSB-EP728855IAAA
CSB-BP728855IAAA
CSB-MP728855IAAA
CSB-EP728855IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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