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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Human Retinoic acid early transcript 1L protein (RAET1L) CSB-YP730141HU
CSB-EP730141HU
CSB-BP730141HU
CSB-MP730141HU
CSB-EP730141HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human SH2 domain-containing adapter protein E (SHE) CSB-YP730142HU
CSB-EP730142HU
CSB-BP730142HU
CSB-MP730142HU
CSB-EP730142HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Doublesex- and mab-3-related transcription factor A1 (DMRTA1) CSB-YP730143HU
CSB-EP730143HU
CSB-BP730143HU
CSB-MP730143HU
CSB-EP730143HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Inactive dual specificity phosphatase 27 (DUSP27), partial CSB-YP730144HU
CSB-EP730144HU
CSB-BP730144HU
CSB-MP730144HU
CSB-EP730144HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein FAM75B (FAM75B) CSB-YP730145HU
CSB-EP730145HU
CSB-BP730145HU
CSB-MP730145HU
CSB-EP730145HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Uncharacterized protein C9orf57 (C9orf57), partial CSB-YP730146HU1
CSB-EP730146HU1
CSB-BP730146HU1
CSB-MP730146HU1
CSB-EP730146HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative cystatin-9-like 2 (CST9L2) CSB-YP730147HU
CSB-EP730147HU
CSB-BP730147HU
CSB-MP730147HU
CSB-EP730147HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog Proteasome subunit beta type-8 (PSMB8) CSB-YP730148DO
CSB-EP730148DO
CSB-BP730148DO
CSB-MP730148DO
CSB-EP730148DO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica CBL-interacting protein kinase 18 (CIPK18) CSB-YP730149OFG
CSB-EP730149OFG
CSB-BP730149OFG
CSB-MP730149OFG
CSB-EP730149OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Vesicular glutamate transporter 2.2 (slc17a6a), partial CSB-YP730150DIL
CSB-EP730150DIL
CSB-BP730150DIL
CSB-MP730150DIL
CSB-EP730150DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bothrops jararaca Snake venom serine protease HS112 CSB-YP730151BUW
CSB-EP730151BUW
CSB-BP730151BUW
CSB-MP730151BUW
CSB-EP730151BUW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus clausii Putative septation protein spoVG (spoVG) CSB-YP730152BAAC
CSB-EP730152BAAC
CSB-BP730152BAAC
CSB-MP730152BAAC
CSB-EP730152BAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus clausii Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP730153BAAC
CSB-EP730153BAAC
CSB-BP730153BAAC
CSB-MP730153BAAC
CSB-EP730153BAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus clausii UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2 (murA2) CSB-YP730154BAAC
CSB-EP730154BAAC
CSB-BP730154BAAC
CSB-MP730154BAAC
CSB-EP730154BAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus clausii Uracil phosphoribosyltransferase (upp) CSB-YP730155BAAC
CSB-EP730155BAAC
CSB-BP730155BAAC
CSB-MP730155BAAC
CSB-EP730155BAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus clausii ATP synthase subunit b (atpF), partial CSB-YP730156BAAC
CSB-EP730156BAAC
CSB-BP730156BAAC
CSB-MP730156BAAC
CSB-EP730156BAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus clausii Heme-degrading monooxygenase isdG (isdG) CSB-YP730157BAAC
CSB-EP730157BAAC
CSB-BP730157BAAC
CSB-MP730157BAAC
CSB-EP730157BAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus clausii Carbon storage regulator homolog (csrA) CSB-YP730158BAAC
CSB-EP730158BAAC
CSB-BP730158BAAC
CSB-MP730158BAAC
CSB-EP730158BAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus clausii ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP730159BAAC
CSB-EP730159BAAC
CSB-BP730159BAAC
CSB-MP730159BAAC
CSB-EP730159BAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus clausii Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP730160BAAC
CSB-EP730160BAAC
CSB-BP730160BAAC
CSB-MP730160BAAC
CSB-EP730160BAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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