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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Kluyveromyces lactis Alpha-1,3/1,6-mannosyltransferase ALG2 (ALG2), partial CSB-YP728383KBK1
CSB-EP728383KBK1
CSB-BP728383KBK1
CSB-MP728383KBK1
CSB-EP728383KBK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Endopolyphosphatase (PPN1), partial CSB-YP728384KBK
CSB-EP728384KBK
CSB-BP728384KBK
CSB-MP728384KBK
CSB-EP728384KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Eukaryotic translation initiation factor 3 subunit G (TIF35) CSB-YP728385KBK
CSB-EP728385KBK
CSB-BP728385KBK
CSB-MP728385KBK
CSB-EP728385KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Chromatin modification-related protein EAF7 (EAF7) CSB-YP728386KBK
CSB-EP728386KBK
CSB-BP728386KBK
CSB-MP728386KBK
CSB-EP728386KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Ceramide synthase subunit LIP1 (LIP1), partial CSB-YP728387KBK
CSB-EP728387KBK
CSB-BP728387KBK
CSB-MP728387KBK
CSB-EP728387KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Golgi apparatus membrane protein TVP38 (TVP38), partial CSB-YP728388KBK1
CSB-EP728388KBK1
CSB-BP728388KBK1
CSB-MP728388KBK1
CSB-EP728388KBK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Protein LST4 (LST4), partial CSB-YP728389KBK
CSB-EP728389KBK
CSB-BP728389KBK
CSB-MP728389KBK
CSB-EP728389KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis ATP-dependent RNA helicase eIF4A (TIF1) CSB-YP728390KBK
CSB-EP728390KBK
CSB-BP728390KBK
CSB-MP728390KBK
CSB-EP728390KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis UDP-N-acetylglucosamine transferase subunit ALG13 (ALG13) CSB-YP728391KBK
CSB-EP728391KBK
CSB-BP728391KBK
CSB-MP728391KBK
CSB-EP728391KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis IMP-specific 5'-nucleotidase 1 (ISN1) CSB-YP728392KBK
CSB-EP728392KBK
CSB-BP728392KBK
CSB-MP728392KBK
CSB-EP728392KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Sensitivity to high expression protein 10 (SHE10), partial CSB-YP728393KBK
CSB-EP728393KBK
CSB-BP728393KBK
CSB-MP728393KBK
CSB-EP728393KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica ATP synthase subunit delta (atpH) CSB-YP728394EAAB
CSB-EP728394EAAB
CSB-BP728394EAAB
CSB-MP728394EAAB
CSB-EP728394EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Phosphate import ATP-binding protein PstB 2 (pstB2) CSB-YP728395EAAB
CSB-EP728395EAAB
CSB-BP728395EAAB
CSB-MP728395EAAB
CSB-EP728395EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Aliphatic sulfonates import ATP-binding protein SsuB (ssuB) CSB-YP728396EAAB
CSB-EP728396EAAB
CSB-BP728396EAAB
CSB-MP728396EAAB
CSB-EP728396EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Sulfurtransferase TusA (tusA) CSB-YP728397EAAB
CSB-EP728397EAAB
CSB-BP728397EAAB
CSB-MP728397EAAB
CSB-EP728397EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Triosephosphate isomerase (tpiA) CSB-YP728398EAAB
CSB-EP728398EAAB
CSB-BP728398EAAB
CSB-MP728398EAAB
CSB-EP728398EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica tRNA (uracil (54)-C (5))-methyltransferase (trmA) CSB-YP728399EAAB
CSB-EP728399EAAB
CSB-BP728399EAAB
CSB-MP728399EAAB
CSB-EP728399EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Putative ECA polymerase (wzyE), partial CSB-YP728400EAAB
CSB-EP728400EAAB
CSB-BP728400EAAB
CSB-MP728400EAAB
CSB-EP728400EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Glycogen debranching enzyme (glgX), partial CSB-YP728401EAAB
CSB-EP728401EAAB
CSB-BP728401EAAB
CSB-MP728401EAAB
CSB-EP728401EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP728402EAAB
CSB-EP728402EAAB
CSB-BP728402EAAB
CSB-MP728402EAAB
CSB-EP728402EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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