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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Yarrowia lipolytica Eukaryotic translation initiation factor 3 subunit I (TIF34) CSB-YP728303YAP
CSB-EP728303YAP
CSB-BP728303YAP
CSB-MP728303YAP
CSB-EP728303YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis ATP-dependent RNA helicase CHL1 (CHL1), partial CSB-YP728304KBK
CSB-EP728304KBK
CSB-BP728304KBK
CSB-MP728304KBK
CSB-EP728304KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis ATPase expression protein 1, mitochondrial (AEP1), partial CSB-YP728305KBK
CSB-EP728305KBK
CSB-BP728305KBK
CSB-MP728305KBK
CSB-EP728305KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Carbamoyl-phosphate synthase arginine-specific small chain (CPA1) CSB-YP728306KBK
CSB-EP728306KBK
CSB-BP728306KBK
CSB-MP728306KBK
CSB-EP728306KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis ATP-dependent RNA helicase DBP2 (DBP2), partial CSB-YP728307KBK
CSB-EP728307KBK
CSB-BP728307KBK
CSB-MP728307KBK
CSB-EP728307KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Translation machinery-associated protein 22 (TMA22) CSB-YP728308KBK
CSB-EP728308KBK
CSB-BP728308KBK
CSB-MP728308KBK
CSB-EP728308KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis 5-amino-6- (5-phosphoribosylamino)uracil reductase (RIB7) CSB-YP728309KBK
CSB-EP728309KBK
CSB-BP728309KBK
CSB-MP728309KBK
CSB-EP728309KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis RNA exonuclease 3 (REX3) CSB-YP728310KBK
CSB-EP728310KBK
CSB-BP728310KBK
CSB-MP728310KBK
CSB-EP728310KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis DNA polymerase epsilon subunit D (DPB4) CSB-YP728311KBK
CSB-EP728311KBK
CSB-BP728311KBK
CSB-MP728311KBK
CSB-EP728311KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Pre-mRNA-splicing factor SLT11 (SLT11) CSB-YP728312KBK
CSB-EP728312KBK
CSB-BP728312KBK
CSB-MP728312KBK
CSB-EP728312KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis DNA repair protein RAD5 (RAD5), partial CSB-YP728313KBK
CSB-EP728313KBK
CSB-BP728313KBK
CSB-MP728313KBK
CSB-EP728313KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Mitochondrial import inner membrane translocase subunit TIM13 (TIM13) CSB-YP728314KBK
CSB-EP728314KBK
CSB-BP728314KBK
CSB-MP728314KBK
CSB-EP728314KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Ribosome-releasing factor 2, mitochondrial (MEF2), partial CSB-YP728315KBK
CSB-EP728315KBK
CSB-BP728315KBK
CSB-MP728315KBK
CSB-EP728315KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Ribosome assembly protein 3 (RSA3) CSB-YP728316KBK
CSB-EP728316KBK
CSB-BP728316KBK
CSB-MP728316KBK
CSB-EP728316KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Required for respiratory growth protein 1, mitochondrial (RRG1) CSB-YP728317KBK
CSB-EP728317KBK
CSB-BP728317KBK
CSB-MP728317KBK
CSB-EP728317KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis mRNA cap guanine-N7 methyltransferase (ABD1) CSB-YP728318KBK
CSB-EP728318KBK
CSB-BP728318KBK
CSB-MP728318KBK
CSB-EP728318KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis mRNA 3'-end-processing protein YTH1 (YTH1) CSB-YP728319KBK
CSB-EP728319KBK
CSB-BP728319KBK
CSB-MP728319KBK
CSB-EP728319KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Vacuolar protein sorting-associated protein 27 (VPS27), partial CSB-YP728320KBK
CSB-EP728320KBK
CSB-BP728320KBK
CSB-MP728320KBK
CSB-EP728320KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Cell division cycle protein 123 (CDC123) CSB-YP728321KBK
CSB-EP728321KBK
CSB-BP728321KBK
CSB-MP728321KBK
CSB-EP728321KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Leucine aminopeptidase 2 (KLLA0F03883g), partial CSB-YP728322KBK
CSB-EP728322KBK
CSB-BP728322KBK
CSB-MP728322KBK
CSB-EP728322KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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