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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Thermus thermophilus Proline--tRNA ligase (proS) CSB-YP729176TNT
CSB-EP729176TNT
CSB-BP729176TNT
CSB-MP729176TNT
CSB-EP729176TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP729177TNT
CSB-EP729177TNT
CSB-BP729177TNT
CSB-MP729177TNT
CSB-EP729177TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP729178TNT
CSB-EP729178TNT
CSB-BP729178TNT
CSB-MP729178TNT
CSB-EP729178TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio WD repeat-containing protein 26 (wdr26), partial CSB-YP729179DIL
CSB-EP729179DIL
CSB-BP729179DIL
CSB-MP729179DIL
CSB-EP729179DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein unc-93 homolog A (unc93a), partial CSB-YP729180DIL
CSB-EP729180DIL
CSB-BP729180DIL
CSB-MP729180DIL
CSB-EP729180DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Uncharacterized protein C2orf73 homolog CSB-YP729181MO
CSB-EP729181MO
CSB-BP729181MO
CSB-MP729181MO
CSB-EP729181MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Biorientation of chromosomes in cell division protein 1 (Bod1) CSB-YP729182MO
CSB-EP729182MO
CSB-BP729182MO
CSB-MP729182MO
CSB-EP729182MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative mitochondrial import inner membrane translocase subunit Tim23B (TIMM23B), partial CSB-YP729183HU1
CSB-EP729183HU1
CSB-BP729183HU1
CSB-MP729183HU1
CSB-EP729183HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse TOM1-like protein 2 (Tom1l2) CSB-YP729184MO
CSB-EP729184MO
CSB-BP729184MO
CSB-MP729184MO
CSB-EP729184MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse DBF4-type zinc finger-containing protein 2 homolog (Zdbf2), partial CSB-YP729185MO
CSB-EP729185MO
CSB-BP729185MO
CSB-MP729185MO
CSB-EP729185MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Active breakpoint cluster region-related protein (Abr), partial CSB-YP729186MO
CSB-EP729186MO
CSB-BP729186MO
CSB-MP729186MO
CSB-EP729186MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Unconventional myosin-Ig (Myo1g), partial CSB-YP729187MO
CSB-EP729187MO
CSB-BP729187MO
CSB-MP729187MO
CSB-EP729187MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Rap1 GTPase-activating protein 2 (Rap1gap2) CSB-YP729188MO
CSB-EP729188MO
CSB-BP729188MO
CSB-MP729188MO
CSB-EP729188MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Zinc finger MYM-type protein 1 (ZMYM1), partial CSB-YP729189HU
CSB-EP729189HU
CSB-BP729189HU
CSB-MP729189HU
CSB-EP729189HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Low density lipoprotein receptor adapter protein 1 (LDLRAP1) CSB-YP729190HU
CSB-EP729190HU
CSB-BP729190HU
CSB-MP729190HU
CSB-EP729190HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Acetyl-CoA carboxylase 1 (Acaca), partial CSB-YP729191MO
CSB-EP729191MO
CSB-BP729191MO
CSB-MP729191MO
CSB-EP729191MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Myosin-1 (Myh1), partial CSB-YP729192MO
CSB-EP729192MO
CSB-BP729192MO
CSB-MP729192MO
CSB-EP729192MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein KIBRA (Wwc1), partial CSB-YP729193MO
CSB-EP729193MO
CSB-BP729193MO
CSB-MP729193MO
CSB-EP729193MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human DNL-type zinc finger protein (DNLZ) CSB-YP729194HU
CSB-EP729194HU
CSB-BP729194HU
CSB-MP729194HU
CSB-EP729194HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human NHS-like protein 1 (NHSL1), partial CSB-YP729195HU
CSB-EP729195HU
CSB-BP729195HU
CSB-MP729195HU
CSB-EP729195HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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