重組蛋白
重組蛋白質(zhì)是分析生物相互作用的有用工具。在研究實驗室中使用了大量的重組蛋白質(zhì),包括商業(yè)蛋白質(zhì)和在特定研究項目中生成的蛋白質(zhì)。它們在許多應(yīng)用中發(fā)揮著關(guān)鍵作用。
如果您正在尋找具有優(yōu)良質(zhì)量(驗證的生物活性,較低的內(nèi)毒素水平和高純度)的重組蛋白質(zhì)來支持您的研究,您來對地方了。
經(jīng)過16多年的發(fā)展,華美生物已經(jīng)建立了從原核到真核的五大系統(tǒng),用于表達重組蛋白質(zhì)。我們擁有一系列蛋白質(zhì)表達和純化技術(shù),包括基于桿狀病毒外膜蛋白(OMP)的蛋白質(zhì)表達技術(shù),以及膜蛋白表達技術(shù)等。
產(chǎn)品特點
- 超過10,000種重組蛋白覆蓋大多數(shù)基因/靶點;
- 5種表達系統(tǒng),包括大腸桿菌(E.coli)、酵母、桿狀病毒感染的昆蟲細胞、哺乳動物細胞和體外大腸桿菌。而體外大腸桿菌表達系統(tǒng)是華美生物的獨特表達系統(tǒng)。與傳統(tǒng)蛋白表達系統(tǒng)相比,體外大腸桿菌省略了許多過程,如質(zhì)粒轉(zhuǎn)化、細胞培養(yǎng)、收集、破碎和離心等,大大提高了工作效率。
- 多種標簽,包括His、GST、Flag、MBP等。
- 廣泛應(yīng)用,包括抗原、細胞分析、結(jié)合分析/蛋白質(zhì)相互作用、細胞培養(yǎng)-無血清培養(yǎng)基、藥物相關(guān)研究、體外酶活性、ELISA標準及其原料、體內(nèi)研究、質(zhì)譜標準、蛋白芯片、SDS-PAGE控制、蛋白質(zhì)結(jié)構(gòu)分析(晶體/電子顯微鏡)。
- 多種物種,包括人類、小鼠、大鼠、兔子、豚鼠、雞、猴子、豬等。
- 無動物副產(chǎn)品的動物源性重組蛋白質(zhì)。華美生物已開發(fā)出無動物副產(chǎn)品接觸的重組蛋白質(zhì)產(chǎn)品線。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì),或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的抗體,請查看華美生物蛋白質(zhì)定制服務(wù)。
通過選擇目標名稱的首字母瀏覽產(chǎn)品。
產(chǎn)品名稱 | 貨號 | 規(guī)格 |
---|---|---|
Recombinant Pongo abelii 60S ribosomal protein L27a (RPL27A) | CSB-YP729096PYX CSB-EP729096PYX CSB-BP729096PYX CSB-MP729096PYX CSB-EP729096PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Membrane-spanning 4-domains subfamily A member 10 (MS4A10), partial | CSB-YP729097PYX1 CSB-EP729097PYX1 CSB-BP729097PYX1 CSB-MP729097PYX1 CSB-EP729097PYX1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii T-complex protein 1 subunit epsilon (CCT5), partial | CSB-YP729098PYX CSB-EP729098PYX CSB-BP729098PYX CSB-MP729098PYX CSB-EP729098PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Catalase (CAT), partial | CSB-YP729099PYX CSB-EP729099PYX CSB-BP729099PYX CSB-MP729099PYX CSB-EP729099PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Interleukin-11 receptor subunit alpha (IL11RA), partial | CSB-YP729100PYX CSB-EP729100PYX CSB-BP729100PYX CSB-MP729100PYX CSB-EP729100PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Hsc70-interacting protein (ST13) | CSB-YP729101PYX CSB-EP729101PYX CSB-BP729101PYX CSB-MP729101PYX CSB-EP729101PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Vitamin K-dependent gamma-carboxylase (GGCX), partial | CSB-YP729102PYX1 CSB-EP729102PYX1 CSB-BP729102PYX1 CSB-MP729102PYX1 CSB-EP729102PYX1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Secreted frizzled-related protein 3 (FRZB) | CSB-YP729103PYX CSB-EP729103PYX CSB-BP729103PYX CSB-MP729103PYX CSB-EP729103PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Cold-inducible RNA-binding protein (CIRBP) | CSB-YP729104PYX CSB-EP729104PYX CSB-BP729104PYX CSB-MP729104PYX CSB-EP729104PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii mRNA export factor (RAE1) | CSB-YP729105PYX CSB-EP729105PYX CSB-BP729105PYX CSB-MP729105PYX CSB-EP729105PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Xaa-Pro dipeptidase (PEPD) | CSB-YP729106PYX CSB-EP729106PYX CSB-BP729106PYX CSB-MP729106PYX CSB-EP729106PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii CKLF-like MARVEL transmembrane domain-containing protein 6 (CMTM6), partial | CSB-YP729107PYX1 CSB-EP729107PYX1 CSB-BP729107PYX1 CSB-MP729107PYX1 CSB-EP729107PYX1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Betaine--homocysteine S-methyltransferase 1 (BHMT) | CSB-YP729108PYX CSB-EP729108PYX CSB-BP729108PYX CSB-MP729108PYX CSB-EP729108PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Nucleoside diphosphate kinase B (NME2) | CSB-YP729109PYX CSB-EP729109PYX CSB-BP729109PYX CSB-MP729109PYX CSB-EP729109PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii FAST kinase domain-containing protein 5 (FASTKD5), partial | CSB-YP729110PYX CSB-EP729110PYX CSB-BP729110PYX CSB-MP729110PYX CSB-EP729110PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii 14-3-3 protein theta (YWHAQ) | CSB-YP729111PYX CSB-EP729111PYX CSB-BP729111PYX CSB-MP729111PYX CSB-EP729111PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Elongation of very long chain fatty acids protein 5 (ELOVL5), partial | CSB-YP729112PYX1 CSB-EP729112PYX1 CSB-BP729112PYX1 CSB-MP729112PYX1 CSB-EP729112PYX1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii ADP-ribosylation factor-like protein 15 (ARL15) | CSB-YP729113PYX CSB-EP729113PYX CSB-BP729113PYX CSB-MP729113PYX CSB-EP729113PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii B7 homolog 6 (B7H6), partial | CSB-YP729114PYX CSB-EP729114PYX CSB-BP729114PYX CSB-MP729114PYX CSB-EP729114PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Protein phosphatase 1 regulatory subunit 7 (PPP1R7) | CSB-YP729115PYX CSB-EP729115PYX CSB-BP729115PYX CSB-MP729115PYX CSB-EP729115PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。
第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條:如何選擇合適的表達載體?
表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。
根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022