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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Acanthamoeba polyphaga mimivirus Putative FNIP repeat-containing protein R636 (MIMI_R636) CSB-YP729378ADAZ
CSB-EP729378ADAZ
CSB-BP729378ADAZ
CSB-MP729378ADAZ
CSB-EP729378ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Putative ankyrin repeat protein R579 (MIMI_R579) CSB-YP729379ADAZ
CSB-EP729379ADAZ
CSB-BP729379ADAZ
CSB-MP729379ADAZ
CSB-EP729379ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein L573 (MIMI_L573), partial CSB-YP729380ADAZ1
CSB-EP729380ADAZ1
CSB-BP729380ADAZ1
CSB-MP729380ADAZ1
CSB-EP729380ADAZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Putative lipocalin R877 (MIMI_R877) CSB-YP729381ADAZ
CSB-EP729381ADAZ
CSB-BP729381ADAZ
CSB-MP729381ADAZ
CSB-EP729381ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Putative BTB/POZ domain-containing protein R842 (MIMI_R842), partial CSB-YP729382ADAZ
CSB-EP729382ADAZ
CSB-BP729382ADAZ
CSB-MP729382ADAZ
CSB-EP729382ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hemitragus jemlahicus Growth/differentiation factor 8 (MSTN) CSB-YP729383HVM
CSB-EP729383HVM
CSB-BP729383HVM
CSB-MP729383HVM
CSB-EP729383HVM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloferax volcanii Proteasome-activating nucleotidase 2 (pan2) CSB-YP729384HTU
CSB-EP729384HTU
CSB-BP729384HTU
CSB-MP729384HTU
CSB-EP729384HTU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse VEGF co-regulated chemokine 1 (Cxcl17) CSB-YP729385MO
CSB-EP729385MO
CSB-BP729385MO
CSB-MP729385MO
CSB-EP729385MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui Isopentenyl-diphosphate Delta-isomerase (idi) CSB-YP729386HTJ
CSB-EP729386HTJ
CSB-BP729386HTJ
CSB-MP729386HTJ
CSB-EP729386HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP729387HTJ
CSB-EP729387HTJ
CSB-BP729387HTJ
CSB-MP729387HTJ
CSB-EP729387HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui 7-cyano-7-deazaguanine tRNA-ribosyltransferase (tgtA) CSB-YP729388HTJ
CSB-EP729388HTJ
CSB-BP729388HTJ
CSB-MP729388HTJ
CSB-EP729388HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui Arginine--tRNA ligase (argS), partial CSB-YP729389HTJ
CSB-EP729389HTJ
CSB-BP729389HTJ
CSB-MP729389HTJ
CSB-EP729389HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui Dihydroorotate dehydrogenase (quinone) (pyrD) CSB-YP729390HTJ
CSB-EP729390HTJ
CSB-BP729390HTJ
CSB-MP729390HTJ
CSB-EP729390HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui Translation initiation factor 2 subunit gamma (eif2g) CSB-YP729391HTJ
CSB-EP729391HTJ
CSB-BP729391HTJ
CSB-MP729391HTJ
CSB-EP729391HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui Isoleucine--tRNA ligase (ileS), partial CSB-YP729392HTJ
CSB-EP729392HTJ
CSB-BP729392HTJ
CSB-MP729392HTJ
CSB-EP729392HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui DNA-directed RNA polymerase subunit A' (rpoA2) CSB-YP729393HTJ
CSB-EP729393HTJ
CSB-BP729393HTJ
CSB-MP729393HTJ
CSB-EP729393HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui Protein CrcB homolog 1 (crcB1), partial CSB-YP729394HTJ1
CSB-EP729394HTJ1
CSB-BP729394HTJ1
CSB-MP729394HTJ1
CSB-EP729394HTJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui Thiamine-phosphate synthase (thiE) CSB-YP729395HTJ
CSB-EP729395HTJ
CSB-BP729395HTJ
CSB-MP729395HTJ
CSB-EP729395HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui Catalase-peroxidase 1 (katG1), partial CSB-YP729396HTJ
CSB-EP729396HTJ
CSB-BP729396HTJ
CSB-MP729396HTJ
CSB-EP729396HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui L-tyrosine decarboxylase (mfnA) CSB-YP729397HTJ
CSB-EP729397HTJ
CSB-BP729397HTJ
CSB-MP729397HTJ
CSB-EP729397HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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