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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Acanthamoeba polyphaga mimivirus Putative serine/threonine-protein kinase L268 (MIMI_L268), partial CSB-YP729318ADAZ
CSB-EP729318ADAZ
CSB-BP729318ADAZ
CSB-MP729318ADAZ
CSB-EP729318ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein L278 (MIMI_L278) CSB-YP729319ADAZ
CSB-EP729319ADAZ
CSB-BP729319ADAZ
CSB-MP729319ADAZ
CSB-EP729319ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein L285 (MIMI_L285) CSB-YP729320ADAZ
CSB-EP729320ADAZ
CSB-BP729320ADAZ
CSB-MP729320ADAZ
CSB-EP729320ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Putative ATP-dependent RNA helicase R290 (MIMI_R290), partial CSB-YP729321ADAZ
CSB-EP729321ADAZ
CSB-BP729321ADAZ
CSB-MP729321ADAZ
CSB-EP729321ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein L237 (MIMI_L237) CSB-YP729322ADAZ
CSB-EP729322ADAZ
CSB-BP729322ADAZ
CSB-MP729322ADAZ
CSB-EP729322ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein R298 (MIMI_R298) CSB-YP729323ADAZ
CSB-EP729323ADAZ
CSB-BP729323ADAZ
CSB-MP729323ADAZ
CSB-EP729323ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein R305 (MIMI_R305), partial CSB-YP729324ADAZ1
CSB-EP729324ADAZ1
CSB-BP729324ADAZ1
CSB-MP729324ADAZ1
CSB-EP729324ADAZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein L309 (MIMI_L309), partial CSB-YP729325ADAZ
CSB-EP729325ADAZ
CSB-BP729325ADAZ
CSB-MP729325ADAZ
CSB-EP729325ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein L792 (MIMI_L792) CSB-YP729326ADAZ
CSB-EP729326ADAZ
CSB-BP729326ADAZ
CSB-MP729326ADAZ
CSB-EP729326ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Putative ankyrin repeat protein R787 (MIMI_R787) CSB-YP729327ADAZ
CSB-EP729327ADAZ
CSB-BP729327ADAZ
CSB-MP729327ADAZ
CSB-EP729327ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein L201 (MIMI_L201) CSB-YP729328ADAZ
CSB-EP729328ADAZ
CSB-BP729328ADAZ
CSB-MP729328ADAZ
CSB-EP729328ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein R197 (MIMI_R197) CSB-YP729329ADAZ
CSB-EP729329ADAZ
CSB-BP729329ADAZ
CSB-MP729329ADAZ
CSB-EP729329ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein R210 (MIMI_R210) CSB-YP729330ADAZ
CSB-EP729330ADAZ
CSB-BP729330ADAZ
CSB-MP729330ADAZ
CSB-EP729330ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein R392 (MIMI_R392) CSB-YP729331ADAZ
CSB-EP729331ADAZ
CSB-BP729331ADAZ
CSB-MP729331ADAZ
CSB-EP729331ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein R710 (MIMI_R710), partial CSB-YP729332ADAZ1
CSB-EP729332ADAZ1
CSB-BP729332ADAZ1
CSB-MP729332ADAZ1
CSB-EP729332ADAZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Putative fucosyltransferase R654 (MIMI_R654), partial CSB-YP729333ADAZ
CSB-EP729333ADAZ
CSB-BP729333ADAZ
CSB-MP729333ADAZ
CSB-EP729333ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Putative zinc metalloproteinase R519 (MIMI_R519), partial CSB-YP729334ADAZ
CSB-EP729334ADAZ
CSB-BP729334ADAZ
CSB-MP729334ADAZ
CSB-EP729334ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein R513 (MIMI_R513), partial CSB-YP729335ADAZ1
CSB-EP729335ADAZ1
CSB-BP729335ADAZ1
CSB-MP729335ADAZ1
CSB-EP729335ADAZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Cytochrome P450-like protein L532 (MIMI_L532), partial CSB-YP729336ADAZ1
CSB-EP729336ADAZ1
CSB-BP729336ADAZ1
CSB-MP729336ADAZ1
CSB-EP729336ADAZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Putative 5'-3' exonuclease R528 (MIMI_R528), partial CSB-YP729337ADAZ
CSB-EP729337ADAZ
CSB-BP729337ADAZ
CSB-MP729337ADAZ
CSB-EP729337ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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