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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Xenopus laevis Histone-arginine methyltransferase CARM1 (carm1), partial CSB-YP730401XBE
CSB-EP730401XBE
CSB-BP730401XBE
CSB-MP730401XBE
CSB-EP730401XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant pH-response transcription factor pacC/RIM101 (pacC), partial CSB-YP730402APN
CSB-EP730402APN
CSB-BP730402APN
CSB-MP730402APN
CSB-EP730402APN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog T-box transcription factor TBX19 (TBX19) CSB-YP730403DO
CSB-EP730403DO
CSB-BP730403DO
CSB-MP730403DO
CSB-EP730403DO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces kudriavzevii Altered inheritance of mitochondria protein 32-1 (AIM32-1) CSB-YP730404SVT
CSB-EP730404SVT
CSB-BP730404SVT
CSB-MP730404SVT
CSB-EP730404SVT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human F-box only protein 31 (FBXO31) CSB-YP730405HU
CSB-EP730405HU
CSB-BP730405HU
CSB-MP730405HU
CSB-EP730405HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Fanconi-associated nuclease 1 homolog (At1g48360), partial CSB-YP730406DOA
CSB-EP730406DOA
CSB-BP730406DOA
CSB-MP730406DOA
CSB-EP730406DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sudan ebolavirus Minor nucleoprotein VP30 (VP30) CSB-YP730407SRE
CSB-EP730407SRE
CSB-BP730407SRE
CSB-MP730407SRE
CSB-EP730407SRE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Rho guanine nucleotide exchange factor 6 (Arhgef6) CSB-YP730408RA
CSB-EP730408RA
CSB-BP730408RA
CSB-MP730408RA
CSB-EP730408RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tetrahymena thermophila Histone H2A.Y (HTAY) CSB-YP730409TKA
CSB-EP730409TKA
CSB-BP730409TKA
CSB-MP730409TKA
CSB-EP730409TKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agelena orientalis U10-agatoxin-Ao1a CSB-YP730410AEQ
CSB-EP730410AEQ
CSB-BP730410AEQ
CSB-MP730410AEQ
CSB-EP730410AEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agelena orientalis U3-agatoxin-Ao1d CSB-YP730411AEQ
CSB-EP730411AEQ
CSB-BP730411AEQ
CSB-MP730411AEQ
CSB-EP730411AEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agelena orientalis U7-agatoxin-Ao1a CSB-YP730412AEQ
CSB-EP730412AEQ
CSB-BP730412AEQ
CSB-MP730412AEQ
CSB-EP730412AEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agelena orientalis U2-agatoxin-Ao1u CSB-YP730413AEQ
CSB-EP730413AEQ
CSB-BP730413AEQ
CSB-MP730413AEQ
CSB-EP730413AEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agelena orientalis U2-agatoxin-Ao1p CSB-YP730414AEQ
CSB-EP730414AEQ
CSB-BP730414AEQ
CSB-MP730414AEQ
CSB-EP730414AEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agelena orientalis U2-agatoxin-Ao1i CSB-YP730415AEQ
CSB-EP730415AEQ
CSB-BP730415AEQ
CSB-MP730415AEQ
CSB-EP730415AEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Gasdermin-A3 (Gsdma3) CSB-YP730416MO
CSB-EP730416MO
CSB-BP730416MO
CSB-MP730416MO
CSB-EP730416MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan paniscus Taste receptor type 2 member 62 (TAS2R62), partial CSB-YP730417EQU1
CSB-EP730417EQU1
CSB-BP730417EQU1
CSB-MP730417EQU1
CSB-EP730417EQU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Probable palmitoyltransferase ZDHHC20 (Zdhhc20), partial CSB-YP730418MO1
CSB-EP730418MO1
CSB-BP730418MO1
CSB-MP730418MO1
CSB-EP730418MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human hepatitis A virus genotype IIA Genome polyprotein CSB-YP730419HCAS
CSB-EP730419HCAS
CSB-BP730419HCAS
CSB-MP730419HCAS
CSB-EP730419HCAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana RNA polymerase II C-terminal domain phosphatase-like 2 (CPL2), partial CSB-YP730420DOA
CSB-EP730420DOA
CSB-BP730420DOA
CSB-MP730420DOA
CSB-EP730420DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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