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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Solanum tuberosum Cytochrome b6-f complex iron-sulfur subunit, chloroplastic (petC), partial CSB-YP731664FIG1
CSB-EP731664FIG1
CSB-BP731664FIG1
CSB-MP731664FIG1
CSB-EP731664FIG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Protein XAP5 CIRCADIAN TIMEKEEPER (XCT) CSB-YP731665OFG
CSB-EP731665OFG
CSB-BP731665OFG
CSB-MP731665OFG
CSB-EP731665OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Zinc finger A20 and AN1 domain-containing stress-associated protein 10 (SAP10) CSB-YP731666OFG
CSB-EP731666OFG
CSB-BP731666OFG
CSB-MP731666OFG
CSB-EP731666OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Cellulose synthase A catalytic subunit 9 [UDP-forming] (CESA9), partial CSB-YP731667OFG
CSB-EP731667OFG
CSB-BP731667OFG
CSB-MP731667OFG
CSB-EP731667OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable protein phosphatase 2C 27 (Os02g0799000, LOC_Os02g55560) CSB-YP731668OFG
CSB-EP731668OFG
CSB-BP731668OFG
CSB-MP731668OFG
CSB-EP731668OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Endoglucanase 24 (Os09g0533900, LOC_Os09g36350), partial CSB-YP731669OFG
CSB-EP731669OFG
CSB-BP731669OFG
CSB-MP731669OFG
CSB-EP731669OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Putative 12-oxophytodienoate reductase 2 (OPR2) CSB-YP731670OFG
CSB-EP731670OFG
CSB-BP731670OFG
CSB-MP731670OFG
CSB-EP731670OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Peroxiredoxin-2E-1, chloroplastic (PRXIIE-1) CSB-YP731671OFG
CSB-EP731671OFG
CSB-BP731671OFG
CSB-MP731671OFG
CSB-EP731671OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica B3 domain-containing protein Os06g0194400 (Os06g0194400, LOC_Os06g09420) CSB-YP731672OFG
CSB-EP731672OFG
CSB-BP731672OFG
CSB-MP731672OFG
CSB-EP731672OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Heme oxygenase 1, chloroplastic (HO1) CSB-YP731673OFG
CSB-EP731673OFG
CSB-BP731673OFG
CSB-MP731673OFG
CSB-EP731673OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Cell division cycle-associated protein 2 (CDCA2), partial CSB-YP731674HU
CSB-EP731674HU
CSB-BP731674HU
CSB-MP731674HU
CSB-EP731674HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Uncharacterized membrane protein C1orf95 (C1orf95), partial CSB-YP731675HU1
CSB-EP731675HU1
CSB-BP731675HU1
CSB-MP731675HU1
CSB-EP731675HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse PWWP domain-containing protein 2A (Pwwp2a) CSB-YP731676MO
CSB-EP731676MO
CSB-BP731676MO
CSB-MP731676MO
CSB-EP731676MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Testis-specific Y-encoded-like protein 5 (Tspyl5) CSB-YP731677MO
CSB-EP731677MO
CSB-BP731677MO
CSB-MP731677MO
CSB-EP731677MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein RIC1 homolog (Kiaa1432), partial CSB-YP731678MO
CSB-EP731678MO
CSB-BP731678MO
CSB-MP731678MO
CSB-EP731678MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse N-acetylglucosamine-1-phosphotransferase subunits alpha/beta (Gnptab), partial CSB-YP731679MO1
CSB-EP731679MO1
CSB-BP731679MO1
CSB-MP731679MO1
CSB-EP731679MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse MORC family CW-type zinc finger protein 2A (Morc2a), partial CSB-YP731680MO
CSB-EP731680MO
CSB-BP731680MO
CSB-MP731680MO
CSB-EP731680MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Sister chromatid cohesion protein PDS5 homolog A (Pds5a), partial CSB-YP731681MO
CSB-EP731681MO
CSB-BP731681MO
CSB-MP731681MO
CSB-EP731681MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Cell division cycle 5-like protein (Cdc5l), partial CSB-YP731682MO
CSB-EP731682MO
CSB-BP731682MO
CSB-MP731682MO
CSB-EP731682MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Raftlin (Rftn1) CSB-YP731683MO
CSB-EP731683MO
CSB-BP731683MO
CSB-MP731683MO
CSB-EP731683MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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