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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Desulfotalea psychrophila tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP731764DAAA
CSB-EP731764DAAA
CSB-BP731764DAAA
CSB-MP731764DAAA
CSB-EP731764DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Protein translocase subunit SecA (secA), partial CSB-YP731765DAAA
CSB-EP731765DAAA
CSB-BP731765DAAA
CSB-MP731765DAAA
CSB-EP731765DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila 50S ribosomal protein L31 (rpmE) CSB-YP731766DAAA
CSB-EP731766DAAA
CSB-BP731766DAAA
CSB-MP731766DAAA
CSB-EP731766DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Flagellar P-ring protein (flgI) CSB-YP731767DAAA
CSB-EP731767DAAA
CSB-BP731767DAAA
CSB-MP731767DAAA
CSB-EP731767DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Translation initiation factor IF-2 (infB), partial CSB-YP731768DAAA
CSB-EP731768DAAA
CSB-BP731768DAAA
CSB-MP731768DAAA
CSB-EP731768DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila 30S ribosomal protein S18 (rpsR) CSB-YP731769DAAA
CSB-EP731769DAAA
CSB-BP731769DAAA
CSB-MP731769DAAA
CSB-EP731769DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Isoleucine--tRNA ligase (ileS), partial CSB-YP731770DAAA
CSB-EP731770DAAA
CSB-BP731770DAAA
CSB-MP731770DAAA
CSB-EP731770DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP731771DAAA
CSB-EP731771DAAA
CSB-BP731771DAAA
CSB-MP731771DAAA
CSB-EP731771DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila LL-diaminopimelate aminotransferase (dapL) CSB-YP731772DAAA
CSB-EP731772DAAA
CSB-BP731772DAAA
CSB-MP731772DAAA
CSB-EP731772DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Aspartate 1-decarboxylase (panD) CSB-YP731773DAAA
CSB-EP731773DAAA
CSB-BP731773DAAA
CSB-MP731773DAAA
CSB-EP731773DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Bifunctional protein FolD (folD) CSB-YP731774DAAA
CSB-EP731774DAAA
CSB-BP731774DAAA
CSB-MP731774DAAA
CSB-EP731774DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila L-seryl-tRNA (Sec) selenium transferase (selA) CSB-YP731775DAAA
CSB-EP731775DAAA
CSB-BP731775DAAA
CSB-MP731775DAAA
CSB-EP731775DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Lipoprotein-releasing system ATP-binding protein LolD (lolD) CSB-YP731776DAAA
CSB-EP731776DAAA
CSB-BP731776DAAA
CSB-MP731776DAAA
CSB-EP731776DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila 50S ribosomal protein L35 (rpmI) CSB-YP731777DAAA
CSB-EP731777DAAA
CSB-BP731777DAAA
CSB-MP731777DAAA
CSB-EP731777DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP731778DAAA
CSB-EP731778DAAA
CSB-BP731778DAAA
CSB-MP731778DAAA
CSB-EP731778DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP731779DAAA
CSB-EP731779DAAA
CSB-BP731779DAAA
CSB-MP731779DAAA
CSB-EP731779DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Ribonuclease PH (rph) CSB-YP731780DAAA
CSB-EP731780DAAA
CSB-BP731780DAAA
CSB-MP731780DAAA
CSB-EP731780DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP731781DAAA
CSB-EP731781DAAA
CSB-BP731781DAAA
CSB-MP731781DAAA
CSB-EP731781DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila 30S ribosomal protein S11 (rpsK) CSB-YP731782DAAA
CSB-EP731782DAAA
CSB-BP731782DAAA
CSB-MP731782DAAA
CSB-EP731782DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila 30S ribosomal protein S14 type Z (rpsZ) CSB-YP731783DAAA
CSB-EP731783DAAA
CSB-BP731783DAAA
CSB-MP731783DAAA
CSB-EP731783DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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