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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Yarrowia lipolytica Protein SYM1 (SYM1), partial CSB-YP731984YAP1
CSB-EP731984YAP1
CSB-BP731984YAP1
CSB-MP731984YAP1
CSB-EP731984YAP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Pre-mRNA-splicing factor CWC24 (CWC24) CSB-YP731985YAP
CSB-EP731985YAP
CSB-BP731985YAP
CSB-MP731985YAP
CSB-EP731985YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Methylthioribulose-1-phosphate dehydratase (MDE1) CSB-YP731986YAP
CSB-EP731986YAP
CSB-BP731986YAP
CSB-MP731986YAP
CSB-EP731986YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Peptidyl-prolyl cis-trans isomerase D (CPR6) CSB-YP731987YAP
CSB-EP731987YAP
CSB-BP731987YAP
CSB-MP731987YAP
CSB-EP731987YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica PAB-dependent poly (A)-specific ribonuclease subunit PAN3 (PAN3), partial CSB-YP731988YAP
CSB-EP731988YAP
CSB-BP731988YAP
CSB-MP731988YAP
CSB-EP731988YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Transcription elongation factor SPT5 (SPT5), partial CSB-YP731989YAP
CSB-EP731989YAP
CSB-BP731989YAP
CSB-MP731989YAP
CSB-EP731989YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Autophagy-related protein 22 (ATG22), partial CSB-YP731990YAP
CSB-EP731990YAP
CSB-BP731990YAP
CSB-MP731990YAP
CSB-EP731990YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Mediator of RNA polymerase II transcription subunit 18 (SRB5) CSB-YP731991YAP
CSB-EP731991YAP
CSB-BP731991YAP
CSB-MP731991YAP
CSB-EP731991YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica ATP-dependent RNA helicase eIF4A (TIF1) CSB-YP731992YAP
CSB-EP731992YAP
CSB-BP731992YAP
CSB-MP731992YAP
CSB-EP731992YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Nucleolar protein 16 (NOP16) CSB-YP731993YAP
CSB-EP731993YAP
CSB-BP731993YAP
CSB-MP731993YAP
CSB-EP731993YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Dol-P-Glc:Glc (2)Man (9)GlcNAc (2)-PP-Dol alpha-1,2-glucosyltransferase (ALG10), partial CSB-YP731994YAP
CSB-EP731994YAP
CSB-BP731994YAP
CSB-MP731994YAP
CSB-EP731994YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Glucose-repressible alcohol dehydrogenase transcriptional effector (CCR4), partial CSB-YP731995YAP
CSB-EP731995YAP
CSB-BP731995YAP
CSB-MP731995YAP
CSB-EP731995YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Enhancer of polycomb-like protein 1 (EPL1), partial CSB-YP731996YAP
CSB-EP731996YAP
CSB-BP731996YAP
CSB-MP731996YAP
CSB-EP731996YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica FK506-binding protein 1 (FPR1) CSB-YP731997YAP
CSB-EP731997YAP
CSB-BP731997YAP
CSB-MP731997YAP
CSB-EP731997YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica RNA exonuclease 3 (REX3), partial CSB-YP731998YAP
CSB-EP731998YAP
CSB-BP731998YAP
CSB-MP731998YAP
CSB-EP731998YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Topoisomerase 1-associated factor 1 (TOF1), partial CSB-YP731999YAP
CSB-EP731999YAP
CSB-BP731999YAP
CSB-MP731999YAP
CSB-EP731999YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Pre-rRNA-processing protein ESF2 (ESF2) CSB-YP732000YAP
CSB-EP732000YAP
CSB-BP732000YAP
CSB-MP732000YAP
CSB-EP732000YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Presequence translocated-associated motor subunit PAM17, mitochondrial (PAM17), partial CSB-YP732001YAP1
CSB-EP732001YAP1
CSB-BP732001YAP1
CSB-MP732001YAP1
CSB-EP732001YAP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Nuclear distribution protein PAC1 (PAC1) CSB-YP732002YAP
CSB-EP732002YAP
CSB-BP732002YAP
CSB-MP732002YAP
CSB-EP732002YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Calcineurin subunit B (CNB1) CSB-YP732003YAP
CSB-EP732003YAP
CSB-BP732003YAP
CSB-MP732003YAP
CSB-EP732003YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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