重組蛋白
重組蛋白質(zhì)是分析生物相互作用的有用工具。在研究實(shí)驗(yàn)室中使用了大量的重組蛋白質(zhì),包括商業(yè)蛋白質(zhì)和在特定研究項(xiàng)目中生成的蛋白質(zhì)。它們在許多應(yīng)用中發(fā)揮著關(guān)鍵作用。
如果您正在尋找具有優(yōu)良質(zhì)量(驗(yàn)證的生物活性,較低的內(nèi)毒素水平和高純度)的重組蛋白質(zhì)來支持您的研究,您來對地方了。
經(jīng)過16多年的發(fā)展,華美生物已經(jīng)建立了從原核到真核的五大系統(tǒng),用于表達(dá)重組蛋白質(zhì)。我們擁有一系列蛋白質(zhì)表達(dá)和純化技術(shù),包括基于桿狀病毒外膜蛋白(OMP)的蛋白質(zhì)表達(dá)技術(shù),以及膜蛋白表達(dá)技術(shù)等。
產(chǎn)品特點(diǎn)
- 超過10,000種重組蛋白覆蓋大多數(shù)基因/靶點(diǎn);
- 5種表達(dá)系統(tǒng),包括大腸桿菌(E.coli)、酵母、桿狀病毒感染的昆蟲細(xì)胞、哺乳動物細(xì)胞和體外大腸桿菌。而體外大腸桿菌表達(dá)系統(tǒng)是華美生物的獨(dú)特表達(dá)系統(tǒng)。與傳統(tǒng)蛋白表達(dá)系統(tǒng)相比,體外大腸桿菌省略了許多過程,如質(zhì)粒轉(zhuǎn)化、細(xì)胞培養(yǎng)、收集、破碎和離心等,大大提高了工作效率。
- 多種標(biāo)簽,包括His、GST、Flag、MBP等。
- 廣泛應(yīng)用,包括抗原、細(xì)胞分析、結(jié)合分析/蛋白質(zhì)相互作用、細(xì)胞培養(yǎng)-無血清培養(yǎng)基、藥物相關(guān)研究、體外酶活性、ELISA標(biāo)準(zhǔn)及其原料、體內(nèi)研究、質(zhì)譜標(biāo)準(zhǔn)、蛋白芯片、SDS-PAGE控制、蛋白質(zhì)結(jié)構(gòu)分析(晶體/電子顯微鏡)。
- 多種物種,包括人類、小鼠、大鼠、兔子、豚鼠、雞、猴子、豬等。
- 無動物副產(chǎn)品的動物源性重組蛋白質(zhì)。華美生物已開發(fā)出無動物副產(chǎn)品接觸的重組蛋白質(zhì)產(chǎn)品線。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì),或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的抗體,請查看華美生物蛋白質(zhì)定制服務(wù)。
通過選擇目標(biāo)名稱的首字母瀏覽產(chǎn)品。
產(chǎn)品名稱 | 貨號 | 規(guī)格 |
---|---|---|
Recombinant Yarrowia lipolytica Protein SYM1 (SYM1), partial | CSB-YP731984YAP1 CSB-EP731984YAP1 CSB-BP731984YAP1 CSB-MP731984YAP1 CSB-EP731984YAP1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Yarrowia lipolytica Pre-mRNA-splicing factor CWC24 (CWC24) | CSB-YP731985YAP CSB-EP731985YAP CSB-BP731985YAP CSB-MP731985YAP CSB-EP731985YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Yarrowia lipolytica Methylthioribulose-1-phosphate dehydratase (MDE1) | CSB-YP731986YAP CSB-EP731986YAP CSB-BP731986YAP CSB-MP731986YAP CSB-EP731986YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Yarrowia lipolytica Peptidyl-prolyl cis-trans isomerase D (CPR6) | CSB-YP731987YAP CSB-EP731987YAP CSB-BP731987YAP CSB-MP731987YAP CSB-EP731987YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Yarrowia lipolytica PAB-dependent poly (A)-specific ribonuclease subunit PAN3 (PAN3), partial | CSB-YP731988YAP CSB-EP731988YAP CSB-BP731988YAP CSB-MP731988YAP CSB-EP731988YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Yarrowia lipolytica Transcription elongation factor SPT5 (SPT5), partial | CSB-YP731989YAP CSB-EP731989YAP CSB-BP731989YAP CSB-MP731989YAP CSB-EP731989YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Yarrowia lipolytica Autophagy-related protein 22 (ATG22), partial | CSB-YP731990YAP CSB-EP731990YAP CSB-BP731990YAP CSB-MP731990YAP CSB-EP731990YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Yarrowia lipolytica Mediator of RNA polymerase II transcription subunit 18 (SRB5) | CSB-YP731991YAP CSB-EP731991YAP CSB-BP731991YAP CSB-MP731991YAP CSB-EP731991YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Yarrowia lipolytica ATP-dependent RNA helicase eIF4A (TIF1) | CSB-YP731992YAP CSB-EP731992YAP CSB-BP731992YAP CSB-MP731992YAP CSB-EP731992YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Yarrowia lipolytica Nucleolar protein 16 (NOP16) | CSB-YP731993YAP CSB-EP731993YAP CSB-BP731993YAP CSB-MP731993YAP CSB-EP731993YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Yarrowia lipolytica Dol-P-Glc:Glc (2)Man (9)GlcNAc (2)-PP-Dol alpha-1,2-glucosyltransferase (ALG10), partial | CSB-YP731994YAP CSB-EP731994YAP CSB-BP731994YAP CSB-MP731994YAP CSB-EP731994YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Yarrowia lipolytica Glucose-repressible alcohol dehydrogenase transcriptional effector (CCR4), partial | CSB-YP731995YAP CSB-EP731995YAP CSB-BP731995YAP CSB-MP731995YAP CSB-EP731995YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Yarrowia lipolytica Enhancer of polycomb-like protein 1 (EPL1), partial | CSB-YP731996YAP CSB-EP731996YAP CSB-BP731996YAP CSB-MP731996YAP CSB-EP731996YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Yarrowia lipolytica FK506-binding protein 1 (FPR1) | CSB-YP731997YAP CSB-EP731997YAP CSB-BP731997YAP CSB-MP731997YAP CSB-EP731997YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Yarrowia lipolytica RNA exonuclease 3 (REX3), partial | CSB-YP731998YAP CSB-EP731998YAP CSB-BP731998YAP CSB-MP731998YAP CSB-EP731998YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Yarrowia lipolytica Topoisomerase 1-associated factor 1 (TOF1), partial | CSB-YP731999YAP CSB-EP731999YAP CSB-BP731999YAP CSB-MP731999YAP CSB-EP731999YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Yarrowia lipolytica Pre-rRNA-processing protein ESF2 (ESF2) | CSB-YP732000YAP CSB-EP732000YAP CSB-BP732000YAP CSB-MP732000YAP CSB-EP732000YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Yarrowia lipolytica Presequence translocated-associated motor subunit PAM17, mitochondrial (PAM17), partial | CSB-YP732001YAP1 CSB-EP732001YAP1 CSB-BP732001YAP1 CSB-MP732001YAP1 CSB-EP732001YAP1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Yarrowia lipolytica Nuclear distribution protein PAC1 (PAC1) | CSB-YP732002YAP CSB-EP732002YAP CSB-BP732002YAP CSB-MP732002YAP CSB-EP732002YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Yarrowia lipolytica Calcineurin subunit B (CNB1) | CSB-YP732003YAP CSB-EP732003YAP CSB-BP732003YAP CSB-MP732003YAP CSB-EP732003YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。
第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條:如何選擇合適的表達(dá)載體?
表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。
根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022