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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Erwinia carotovora subsp. atroseptica 3-ketoacyl-CoA thiolase (fadA) CSB-YP732184EAAB
CSB-EP732184EAAB
CSB-BP732184EAAB
CSB-MP732184EAAB
CSB-EP732184EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Acetylglutamate kinase (argB) CSB-YP732185EAAB
CSB-EP732185EAAB
CSB-BP732185EAAB
CSB-MP732185EAAB
CSB-EP732185EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) CSB-YP732186EAAB
CSB-EP732186EAAB
CSB-BP732186EAAB
CSB-MP732186EAAB
CSB-EP732186EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica L-lactate dehydrogenase [cytochrome] (lldD) CSB-YP732187EAAB
CSB-EP732187EAAB
CSB-BP732187EAAB
CSB-MP732187EAAB
CSB-EP732187EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Glyoxylate/hydroxypyruvate reductase B (ghrB) CSB-YP732188EAAB
CSB-EP732188EAAB
CSB-BP732188EAAB
CSB-MP732188EAAB
CSB-EP732188EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Probable GTP-binding protein EngB (engB) CSB-YP732189EAAB
CSB-EP732189EAAB
CSB-BP732189EAAB
CSB-MP732189EAAB
CSB-EP732189EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana BTB/POZ domain-containing protein At4g08455 (At4g08455) CSB-YP732190DOA
CSB-EP732190DOA
CSB-BP732190DOA
CSB-MP732190DOA
CSB-EP732190DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio RNA pseudouridylate synthase domain-containing protein 4 (rpusd4) CSB-YP732191DIL
CSB-EP732191DIL
CSB-BP732191DIL
CSB-MP732191DIL
CSB-EP732191DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Probable phosphatase phospho1 (phospho1) CSB-YP732192DIL
CSB-EP732192DIL
CSB-BP732192DIL
CSB-MP732192DIL
CSB-EP732192DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio N-alpha-acetyltransferase 50 (naa50) CSB-YP732193DIL
CSB-EP732193DIL
CSB-BP732193DIL
CSB-MP732193DIL
CSB-EP732193DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Bcl-2-related ovarian killer protein homolog A (boka) CSB-YP732194DIL
CSB-EP732194DIL
CSB-BP732194DIL
CSB-MP732194DIL
CSB-EP732194DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis LysM and putative peptidoglycan-binding domain-containing protein 4 (lysmd4), partial CSB-YP732195XBE
CSB-EP732195XBE
CSB-BP732195XBE
CSB-MP732195XBE
CSB-EP732195XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Low affinity cationic amino acid transporter 2 (slc7a2), partial CSB-YP732196XBE
CSB-EP732196XBE
CSB-BP732196XBE
CSB-MP732196XBE
CSB-EP732196XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein FAM69A (fam69a), partial CSB-YP732197XBE
CSB-EP732197XBE
CSB-BP732197XBE
CSB-MP732197XBE
CSB-EP732197XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis UPF0668 protein C10orf76 homolog, partial CSB-YP732198XBE1
CSB-EP732198XBE1
CSB-BP732198XBE1
CSB-MP732198XBE1
CSB-EP732198XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis BTB/POZ domain-containing protein kctd15 (kctd15) CSB-YP732199XBE
CSB-EP732199XBE
CSB-BP732199XBE
CSB-MP732199XBE
CSB-EP732199XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Coiled-coil domain-containing protein 124-B (ccdc124-b) CSB-YP732200XBE
CSB-EP732200XBE
CSB-BP732200XBE
CSB-MP732200XBE
CSB-EP732200XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Xenotropic and polytropic retrovirus receptor 1 homolog (xpr1), partial CSB-YP732201XBE
CSB-EP732201XBE
CSB-BP732201XBE
CSB-MP732201XBE
CSB-EP732201XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis DnaJ homolog subfamily C member 15 (dnajc15), partial CSB-YP732202XBF1
CSB-EP732202XBF1
CSB-BP732202XBF1
CSB-MP732202XBF1
CSB-EP732202XBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis WD repeat-containing protein 37 (wdr37) CSB-YP732203XBE
CSB-EP732203XBE
CSB-BP732203XBE
CSB-MP732203XBE
CSB-EP732203XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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