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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Bacillus clausii Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP733217BAAC
CSB-EP733217BAAC
CSB-BP733217BAAC
CSB-MP733217BAAC
CSB-EP733217BAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus clausii GTPase obg (obg) CSB-YP733218BAAC
CSB-EP733218BAAC
CSB-BP733218BAAC
CSB-MP733218BAAC
CSB-EP733218BAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus clausii Sugar fermentation stimulation protein homolog (sfsA) CSB-YP733219BAAC
CSB-EP733219BAAC
CSB-BP733219BAAC
CSB-MP733219BAAC
CSB-EP733219BAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus clausii Heptaprenylglyceryl phosphate synthase (pcrB) CSB-YP733220BAAC
CSB-EP733220BAAC
CSB-BP733220BAAC
CSB-MP733220BAAC
CSB-EP733220BAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus clausii GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP733221BAAC
CSB-EP733221BAAC
CSB-BP733221BAAC
CSB-MP733221BAAC
CSB-EP733221BAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus clausii Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP733222BAAC
CSB-EP733222BAAC
CSB-BP733222BAAC
CSB-MP733222BAAC
CSB-EP733222BAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus clausii Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP733223BAAC
CSB-EP733223BAAC
CSB-BP733223BAAC
CSB-MP733223BAAC
CSB-EP733223BAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus clausii Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP733224BAAC
CSB-EP733224BAAC
CSB-BP733224BAAC
CSB-MP733224BAAC
CSB-EP733224BAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus clausii Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP733225BAAC
CSB-EP733225BAAC
CSB-BP733225BAAC
CSB-MP733225BAAC
CSB-EP733225BAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus clausii Methylmalonate semialdehyde dehydrogenase [acylating] 1 (iolA1) CSB-YP733226BAAC
CSB-EP733226BAAC
CSB-BP733226BAAC
CSB-MP733226BAAC
CSB-EP733226BAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus clausii Bifunctional enzyme rhaA/rhaB (rhaAB), partial CSB-YP733227BAAC
CSB-EP733227BAAC
CSB-BP733227BAAC
CSB-MP733227BAAC
CSB-EP733227BAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus clausii 30S ribosomal protein S9 (rpsI) CSB-YP733228BAAC
CSB-EP733228BAAC
CSB-BP733228BAAC
CSB-MP733228BAAC
CSB-EP733228BAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus clausii 50S ribosomal protein L17 (rplQ) CSB-YP733229BAAC
CSB-EP733229BAAC
CSB-BP733229BAAC
CSB-MP733229BAAC
CSB-EP733229BAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus clausii Translation initiation factor IF-1 (infA) CSB-YP733230BAAC
CSB-EP733230BAAC
CSB-BP733230BAAC
CSB-MP733230BAAC
CSB-EP733230BAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus clausii 50S ribosomal protein L5 (rplE) CSB-YP733231BAAC
CSB-EP733231BAAC
CSB-BP733231BAAC
CSB-MP733231BAAC
CSB-EP733231BAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus clausii 50S ribosomal protein L4 (rplD) CSB-YP733232BAAC
CSB-EP733232BAAC
CSB-BP733232BAAC
CSB-MP733232BAAC
CSB-EP733232BAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus clausii Elongation factor G (fusA), partial CSB-YP733233BAAC
CSB-EP733233BAAC
CSB-BP733233BAAC
CSB-MP733233BAAC
CSB-EP733233BAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus clausii Cysteine--tRNA ligase (cysS) CSB-YP733234BAAC
CSB-EP733234BAAC
CSB-BP733234BAAC
CSB-MP733234BAAC
CSB-EP733234BAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus clausii Lysine--tRNA ligase (lysS), partial CSB-YP733235BAAC
CSB-EP733235BAAC
CSB-BP733235BAAC
CSB-MP733235BAAC
CSB-EP733235BAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus clausii Chromosomal replication initiator protein DnaA (dnaA) CSB-YP733236BAAC
CSB-EP733236BAAC
CSB-BP733236BAAC
CSB-MP733236BAAC
CSB-EP733236BAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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