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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pongo abelii Probable palmitoyltransferase ZDHHC21 (ZDHHC21), partial CSB-YP732734PYX1
CSB-EP732734PYX1
CSB-BP732734PYX1
CSB-MP732734PYX1
CSB-EP732734PYX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Protein FAM214B (FAM214B), partial CSB-YP732735PYX
CSB-EP732735PYX
CSB-BP732735PYX
CSB-MP732735PYX
CSB-EP732735PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Isovaleryl-CoA dehydrogenase, mitochondrial (IVD) CSB-YP732736PYX
CSB-EP732736PYX
CSB-BP732736PYX
CSB-MP732736PYX
CSB-EP732736PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii E3 ubiquitin-protein ligase NEDD4-like (NEDD4L), partial CSB-YP732737PYX
CSB-EP732737PYX
CSB-BP732737PYX
CSB-MP732737PYX
CSB-EP732737PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Diablo homolog, mitochondrial (DIABLO) CSB-YP732738PYX
CSB-EP732738PYX
CSB-BP732738PYX
CSB-MP732738PYX
CSB-EP732738PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii PDZ domain-containing protein 7 (PDZD7), partial CSB-YP732739PYX
CSB-EP732739PYX
CSB-BP732739PYX
CSB-MP732739PYX
CSB-EP732739PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Trafficking protein particle complex subunit 2-like protein (TRAPPC2L) CSB-YP732740PYX
CSB-EP732740PYX
CSB-BP732740PYX
CSB-MP732740PYX
CSB-EP732740PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Chitinase-3-like protein 1 (CHI3L1) CSB-YP732741PYX
CSB-EP732741PYX
CSB-BP732741PYX
CSB-MP732741PYX
CSB-EP732741PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii ATP synthase subunit gamma, mitochondrial (ATP5C1) CSB-YP732742PYX
CSB-EP732742PYX
CSB-BP732742PYX
CSB-MP732742PYX
CSB-EP732742PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Lysosomal protein NCU-G1, partial CSB-YP732743PYX
CSB-EP732743PYX
CSB-BP732743PYX
CSB-MP732743PYX
CSB-EP732743PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii RING finger protein C14orf164 homolog CSB-YP732744PYX
CSB-EP732744PYX
CSB-BP732744PYX
CSB-MP732744PYX
CSB-EP732744PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Nucleoporin NDC1 (TMEM48), partial CSB-YP732745PYX1
CSB-EP732745PYX1
CSB-BP732745PYX1
CSB-MP732745PYX1
CSB-EP732745PYX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii WD repeat-containing protein 89 (WDR89) CSB-YP732746PYX
CSB-EP732746PYX
CSB-BP732746PYX
CSB-MP732746PYX
CSB-EP732746PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Peroxisomal acyl-coenzyme A oxidase 1 (ACOX1), partial CSB-YP732747PYX
CSB-EP732747PYX
CSB-BP732747PYX
CSB-MP732747PYX
CSB-EP732747PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Nuclear protein MDM1 (MDM1), partial CSB-YP732748PYX
CSB-EP732748PYX
CSB-BP732748PYX
CSB-MP732748PYX
CSB-EP732748PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Solute carrier family 22 member 24 (SLC22A24), partial CSB-YP732749PYX1
CSB-EP732749PYX1
CSB-BP732749PYX1
CSB-MP732749PYX1
CSB-EP732749PYX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii H/ACA ribonucleoprotein complex subunit 2 (NHP2) CSB-YP732750PYX
CSB-EP732750PYX
CSB-BP732750PYX
CSB-MP732750PYX
CSB-EP732750PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii 39S ribosomal protein L17, mitochondrial (MRPL17) CSB-YP732751PYX
CSB-EP732751PYX
CSB-BP732751PYX
CSB-MP732751PYX
CSB-EP732751PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Clavesin-1 (CLVS1) CSB-YP732752PYX
CSB-EP732752PYX
CSB-BP732752PYX
CSB-MP732752PYX
CSB-EP732752PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii TM2 domain-containing protein 2 (TM2D2), partial CSB-YP732753PYX1
CSB-EP732753PYX1
CSB-BP732753PYX1
CSB-MP732753PYX1
CSB-EP732753PYX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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