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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pongo abelii T-complex protein 1 subunit zeta (CCT6), partial CSB-YP732754PYX
CSB-EP732754PYX
CSB-BP732754PYX
CSB-MP732754PYX
CSB-EP732754PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Ribosomal L1 domain-containing protein 1 (RSL1D1) CSB-YP732755PYX
CSB-EP732755PYX
CSB-BP732755PYX
CSB-MP732755PYX
CSB-EP732755PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii FAD synthase (FLAD1) CSB-YP732756PYX
CSB-EP732756PYX
CSB-BP732756PYX
CSB-MP732756PYX
CSB-EP732756PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii PEST proteolytic signal-containing nuclear protein (PCNP) CSB-YP732757PYX
CSB-EP732757PYX
CSB-BP732757PYX
CSB-MP732757PYX
CSB-EP732757PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Synaptotagmin-10 (SYT10), partial CSB-YP732758PYX
CSB-EP732758PYX
CSB-BP732758PYX
CSB-MP732758PYX
CSB-EP732758PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Cytochrome P450 4V2 (CYP4V2), partial CSB-YP732759PYX1
CSB-EP732759PYX1
CSB-BP732759PYX1
CSB-MP732759PYX1
CSB-EP732759PYX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii 60S ribosomal protein L4 (RPL4) CSB-YP732760PYX
CSB-EP732760PYX
CSB-BP732760PYX
CSB-MP732760PYX
CSB-EP732760PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Histone H4 CSB-YP732761PYX
CSB-EP732761PYX
CSB-BP732761PYX
CSB-MP732761PYX
CSB-EP732761PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Tumor protein D54 (TPD52L2) CSB-YP732762PYX
CSB-EP732762PYX
CSB-BP732762PYX
CSB-MP732762PYX
CSB-EP732762PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Transcription factor SOX-6 (SOX6), partial CSB-YP732763PYX
CSB-EP732763PYX
CSB-BP732763PYX
CSB-MP732763PYX
CSB-EP732763PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Alpha-1-antitrypsin (SERPINA1) CSB-YP732764PYX
CSB-EP732764PYX
CSB-BP732764PYX
CSB-MP732764PYX
CSB-EP732764PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii ADP-sugar pyrophosphatase (NUDT5) CSB-YP732765PYX
CSB-EP732765PYX
CSB-BP732765PYX
CSB-MP732765PYX
CSB-EP732765PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Nuclear receptor subfamily 2 group C member 1 (NR2C1), partial CSB-YP732766PYX
CSB-EP732766PYX
CSB-BP732766PYX
CSB-MP732766PYX
CSB-EP732766PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Potassium channel subfamily K member 1 (KCNK1), partial CSB-YP732767PYX1
CSB-EP732767PYX1
CSB-BP732767PYX1
CSB-MP732767PYX1
CSB-EP732767PYX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Golgi-associated PDZ and coiled-coil motif-containing protein (GOPC) CSB-YP732768PYX
CSB-EP732768PYX
CSB-BP732768PYX
CSB-MP732768PYX
CSB-EP732768PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Cyclin-L1 (CCNL1) CSB-YP732769PYX
CSB-EP732769PYX
CSB-BP732769PYX
CSB-MP732769PYX
CSB-EP732769PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Contactin-associated protein-like 2 (CNTNAP2), partial CSB-YP732770PYX
CSB-BP732770PYX
CSB-MP732770PYX
CSB-EP732770PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Probable E3 ubiquitin-protein ligase HECTD2 (HECTD2), partial CSB-YP732771PYX
CSB-EP732771PYX
CSB-BP732771PYX
CSB-MP732771PYX
CSB-EP732771PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Cysteine and histidine-rich domain-containing protein 1 (CHORDC1) CSB-YP732772PYX
CSB-EP732772PYX
CSB-BP732772PYX
CSB-MP732772PYX
CSB-EP732772PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii WASH complex subunit FAM21 (FAM21), partial CSB-YP732773PYX
CSB-EP732773PYX
CSB-BP732773PYX
CSB-MP732773PYX
CSB-EP732773PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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