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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Legionella pneumophila tRNA (Ile)-lysidine synthase (tilS) CSB-YP733277LAAA
CSB-EP733277LAAA
CSB-BP733277LAAA
CSB-MP733277LAAA
CSB-EP733277LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila N utilization substance protein B homolog (nusB) CSB-YP733278LAAA
CSB-EP733278LAAA
CSB-BP733278LAAA
CSB-MP733278LAAA
CSB-EP733278LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Phosphopentomutase (deoB) CSB-YP733279LAAA
CSB-EP733279LAAA
CSB-BP733279LAAA
CSB-MP733279LAAA
CSB-EP733279LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Putative Holliday junction resolvase (lpl0621) CSB-YP733280LAAA
CSB-EP733280LAAA
CSB-BP733280LAAA
CSB-MP733280LAAA
CSB-EP733280LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila DNA polymerase IV (dinB) CSB-YP733281LAAA
CSB-EP733281LAAA
CSB-BP733281LAAA
CSB-MP733281LAAA
CSB-EP733281LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP733282LAAA
CSB-EP733282LAAA
CSB-BP733282LAAA
CSB-MP733282LAAA
CSB-EP733282LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila 30S ribosomal protein S13 (rpsM) CSB-YP733283LAAA
CSB-EP733283LAAA
CSB-BP733283LAAA
CSB-MP733283LAAA
CSB-EP733283LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila 30S ribosomal protein S14 (rpsN) CSB-YP733284LAAA
CSB-EP733284LAAA
CSB-BP733284LAAA
CSB-MP733284LAAA
CSB-EP733284LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila 50S ribosomal protein L29 (rpmC) CSB-YP733285LAAA
CSB-EP733285LAAA
CSB-BP733285LAAA
CSB-MP733285LAAA
CSB-EP733285LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila 30S ribosomal protein S19 (rpsS) CSB-YP733286LAAA
CSB-EP733286LAAA
CSB-BP733286LAAA
CSB-MP733286LAAA
CSB-EP733286LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila 50S ribosomal protein L7/L12 (rplL) CSB-YP733287LAAA
CSB-EP733287LAAA
CSB-BP733287LAAA
CSB-MP733287LAAA
CSB-EP733287LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila 50S ribosomal protein L11 (rplK) CSB-YP733288LAAA
CSB-EP733288LAAA
CSB-BP733288LAAA
CSB-MP733288LAAA
CSB-EP733288LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila DNA gyrase inhibitor YacG (yacG) CSB-YP733289LAAA
CSB-EP733289LAAA
CSB-BP733289LAAA
CSB-MP733289LAAA
CSB-EP733289LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP733290LAAA
CSB-EP733290LAAA
CSB-BP733290LAAA
CSB-MP733290LAAA
CSB-EP733290LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila UvrABC system protein B (uvrB), partial CSB-YP733291LAAA
CSB-EP733291LAAA
CSB-BP733291LAAA
CSB-MP733291LAAA
CSB-EP733291LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila tRNA modification GTPase MnmE (mnmE) CSB-YP733292LAAB
CSB-EP733292LAAB
CSB-BP733292LAAB
CSB-MP733292LAAB
CSB-EP733292LAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Deoxycytidine triphosphate deaminase (dcd) CSB-YP733293LAAB
CSB-EP733293LAAB
CSB-BP733293LAAB
CSB-MP733293LAAB
CSB-EP733293LAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Sec-independent protein translocase protein TatA (tatA), partial CSB-YP733294LAAB
CSB-EP733294LAAB
CSB-BP733294LAAB
CSB-MP733294LAAB
CSB-EP733294LAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Methionine--tRNA ligase (metG), partial CSB-YP733295LAAB
CSB-EP733295LAAB
CSB-BP733295LAAB
CSB-MP733295LAAB
CSB-EP733295LAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Shikimate dehydrogenase (aroE) CSB-YP733296LAAB
CSB-EP733296LAAB
CSB-BP733296LAAB
CSB-MP733296LAAB
CSB-EP733296LAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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