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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Legionella pneumophila Protein grpE (grpE) CSB-YP733257LAAA
CSB-EP733257LAAA
CSB-BP733257LAAA
CSB-MP733257LAAA
CSB-EP733257LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Probable Fe (2+)-trafficking protein (lpl1891) CSB-YP733258LAAA
CSB-EP733258LAAA
CSB-BP733258LAAA
CSB-MP733258LAAA
CSB-EP733258LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP733259LAAA
CSB-EP733259LAAA
CSB-BP733259LAAA
CSB-MP733259LAAA
CSB-EP733259LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila DNA mismatch repair protein MutS (mutS), partial CSB-YP733260LAAA
CSB-EP733260LAAA
CSB-BP733260LAAA
CSB-MP733260LAAA
CSB-EP733260LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Outer-membrane lipoprotein carrier protein (lolA) CSB-YP733261LAAA
CSB-EP733261LAAA
CSB-BP733261LAAA
CSB-MP733261LAAA
CSB-EP733261LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Elongation factor Ts (tsf) CSB-YP733262LAAA
CSB-EP733262LAAA
CSB-BP733262LAAA
CSB-MP733262LAAA
CSB-EP733262LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila N-succinylarginine dihydrolase (astB) CSB-YP733263LAAA
CSB-EP733263LAAA
CSB-BP733263LAAA
CSB-MP733263LAAA
CSB-EP733263LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Dephospho-CoA kinase (coaE) CSB-YP733264LAAA
CSB-EP733264LAAA
CSB-BP733264LAAA
CSB-MP733264LAAA
CSB-EP733264LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP733265LAAA
CSB-EP733265LAAA
CSB-BP733265LAAA
CSB-MP733265LAAA
CSB-EP733265LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP733266LAAA
CSB-EP733266LAAA
CSB-BP733266LAAA
CSB-MP733266LAAA
CSB-EP733266LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP733267LAAA
CSB-EP733267LAAA
CSB-BP733267LAAA
CSB-MP733267LAAA
CSB-EP733267LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP733268LAAA
CSB-EP733268LAAA
CSB-BP733268LAAA
CSB-MP733268LAAA
CSB-EP733268LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP733269LAAA
CSB-EP733269LAAA
CSB-BP733269LAAA
CSB-MP733269LAAA
CSB-EP733269LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Leucine--tRNA ligase (leuS), partial CSB-YP733270LAAA
CSB-EP733270LAAA
CSB-BP733270LAAA
CSB-MP733270LAAA
CSB-EP733270LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB) CSB-YP733271LAAA
CSB-EP733271LAAA
CSB-BP733271LAAA
CSB-MP733271LAAA
CSB-EP733271LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Tryptophan synthase beta chain (trpB) CSB-YP733272LAAA
CSB-EP733272LAAA
CSB-BP733272LAAA
CSB-MP733272LAAA
CSB-EP733272LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Probable intracellular septation protein A (lpl1255), partial CSB-YP733273LAAA1
CSB-EP733273LAAA1
CSB-BP733273LAAA1
CSB-MP733273LAAA1
CSB-EP733273LAAA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP733274LAAA
CSB-EP733274LAAA
CSB-BP733274LAAA
CSB-MP733274LAAA
CSB-EP733274LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Shikimate kinase (aroK) CSB-YP733275LAAA
CSB-EP733275LAAA
CSB-BP733275LAAA
CSB-MP733275LAAA
CSB-EP733275LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP733276LAAA
CSB-EP733276LAAA
CSB-BP733276LAAA
CSB-MP733276LAAA
CSB-EP733276LAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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