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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0018 (MJ0018), partial CSB-YP733657MRU1
CSB-EP733657MRU1
CSB-BP733657MRU1
CSB-MP733657MRU1
CSB-EP733657MRU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0021 (MJ0021) CSB-YP733658MRU
CSB-EP733658MRU
CSB-BP733658MRU
CSB-MP733658MRU
CSB-EP733658MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii tRNA (cytosine (48)-C (5))-methyltransferase (trm4) CSB-YP733659MRU
CSB-EP733659MRU
CSB-BP733659MRU
CSB-MP733659MRU
CSB-EP733659MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii UPF0051 protein MJ0034 (MJ0034) CSB-YP733660MRU
CSB-EP733660MRU
CSB-BP733660MRU
CSB-MP733660MRU
CSB-EP733660MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Riboflavin kinase (ribK) CSB-YP733661MRU
CSB-EP733661MRU
CSB-BP733661MRU
CSB-MP733661MRU
CSB-EP733661MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Probable acetylglutamate kinase (argB) CSB-YP733662MRU
CSB-EP733662MRU
CSB-BP733662MRU
CSB-MP733662MRU
CSB-EP733662MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botulinum neurotoxin type G (botG), partial CSB-YP733663CLQ
CSB-EP733663CLQ
CSB-BP733663CLQ
CSB-MP733663CLQ
CSB-EP733663CLQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylococcus capsulatus Protein RnfH (rnfH) CSB-YP733664MFM
CSB-EP733664MFM
CSB-BP733664MFM
CSB-MP733664MFM
CSB-EP733664MFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylococcus capsulatus Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP733665MFM
CSB-EP733665MFM
CSB-BP733665MFM
CSB-MP733665MFM
CSB-EP733665MFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylococcus capsulatus Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP733666MFM
CSB-EP733666MFM
CSB-BP733666MFM
CSB-MP733666MFM
CSB-EP733666MFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylococcus capsulatus UPF0182 protein MCA2716 (MCA2716), partial CSB-YP733667MFM
CSB-EP733667MFM
CSB-BP733667MFM
CSB-MP733667MFM
CSB-EP733667MFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guinea pig Potassium voltage-gated channel subfamily E member 1 (KCNE1), partial CSB-YP733668GU
CSB-EP733668GU
CSB-BP733668GU
CSB-MP733668GU
CSB-EP733668GU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus Scavenger receptor class B member 1 (SCARB1), partial CSB-YP733669DXU
CSB-EP733669DXU
CSB-BP733669DXU
CSB-MP733669DXU
CSB-EP733669DXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Caspase-3 (CASP3) CSB-YP733670MRG
CSB-EP733670MRG
CSB-BP733670MRG
CSB-MP733670MRG
CSB-EP733670MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus Mannose-P-dolichol utilization defect 1 protein (MPDU1), partial CSB-YP733671DXU1
CSB-EP733671DXU1
CSB-BP733671DXU1
CSB-MP733671DXU1
CSB-EP733671DXU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guinea pig Interleukin-1 alpha (IL1A) CSB-YP733672GU
CSB-EP733672GU
CSB-BP733672GU
CSB-MP733672GU
CSB-EP733672GU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guinea pig Acrosin-binding protein (ACRBP), partial CSB-YP733673GU
CSB-EP733673GU
CSB-BP733673GU
CSB-MP733673GU
CSB-EP733673GU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylococcus capsulatus Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP733674MFM
CSB-EP733674MFM
CSB-BP733674MFM
CSB-MP733674MFM
CSB-EP733674MFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylococcus capsulatus Enolase 2 (eno2) CSB-YP733675MFM
CSB-EP733675MFM
CSB-BP733675MFM
CSB-MP733675MFM
CSB-EP733675MFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylococcus capsulatus Tryptophan synthase alpha chain (trpA) CSB-YP733676MFM
CSB-EP733676MFM
CSB-BP733676MFM
CSB-MP733676MFM
CSB-EP733676MFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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