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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Yarrowia lipolytica Translation machinery-associated protein 22 (TMA22) CSB-YP735060YAP
CSB-EP735060YAP
CSB-BP735060YAP
CSB-MP735060YAP
CSB-EP735060YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica High osmolarity signaling protein SHO1 (SHO1), partial CSB-YP735061YAP1
CSB-EP735061YAP1
CSB-BP735061YAP1
CSB-MP735061YAP1
CSB-EP735061YAP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Mitochondrial distribution and morphology protein 10 (MDM10) CSB-YP735062YAP
CSB-EP735062YAP
CSB-BP735062YAP
CSB-MP735062YAP
CSB-EP735062YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Ribonuclease T2-like (RNY1) CSB-YP735063YAP
CSB-EP735063YAP
CSB-BP735063YAP
CSB-MP735063YAP
CSB-EP735063YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Restriction of telomere capping protein 5 (RTC5), partial CSB-YP735064YAP
CSB-EP735064YAP
CSB-BP735064YAP
CSB-MP735064YAP
CSB-EP735064YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica GPN-loop GTPase 3 homolog YALI0C20317g (YALI0C20317g) CSB-YP735065YAP
CSB-EP735065YAP
CSB-BP735065YAP
CSB-MP735065YAP
CSB-EP735065YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Survival factor 1 (SVF1) CSB-YP735066YAP
CSB-EP735066YAP
CSB-BP735066YAP
CSB-MP735066YAP
CSB-EP735066YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Arginine N-methyltransferase 2 (RMT2) CSB-YP735067YAP
CSB-EP735067YAP
CSB-BP735067YAP
CSB-MP735067YAP
CSB-EP735067YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Mannose-1-phosphate guanyltransferase (MPG1) CSB-YP735068YAP
CSB-EP735068YAP
CSB-BP735068YAP
CSB-MP735068YAP
CSB-EP735068YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica RNA polymerase II transcription factor B subunit 4 (TFB4) CSB-YP735069YAP
CSB-EP735069YAP
CSB-BP735069YAP
CSB-MP735069YAP
CSB-EP735069YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Glutamyl-tRNA (Gln) amidotransferase subunit F, mitochondrial (YALI0B23386g) CSB-YP735070YAP
CSB-EP735070YAP
CSB-BP735070YAP
CSB-MP735070YAP
CSB-EP735070YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Rhomboid protein 2 (RBD2), partial CSB-YP735071YAP1
CSB-EP735071YAP1
CSB-BP735071YAP1
CSB-MP735071YAP1
CSB-EP735071YAP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica RNA exonuclease 4 (REX4) CSB-YP735072YAP
CSB-EP735072YAP
CSB-BP735072YAP
CSB-MP735072YAP
CSB-EP735072YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Ribosome biogenesis protein NSA1 (NSA1) CSB-YP735073YAP
CSB-EP735073YAP
CSB-BP735073YAP
CSB-MP735073YAP
CSB-EP735073YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica DASH complex subunit DAD1 (DAD1) CSB-YP735074YAP
CSB-EP735074YAP
CSB-BP735074YAP
CSB-MP735074YAP
CSB-EP735074YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Ribosome biogenesis protein YTM1 (YTM1), partial CSB-YP735075YAP
CSB-EP735075YAP
CSB-BP735075YAP
CSB-MP735075YAP
CSB-EP735075YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Chromatin modification-related protein EAF7 (EAF7) CSB-YP735076YAP
CSB-EP735076YAP
CSB-BP735076YAP
CSB-MP735076YAP
CSB-EP735076YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Sulfate adenylyltransferase (MET3), partial CSB-YP735077YAP
CSB-EP735077YAP
CSB-BP735077YAP
CSB-MP735077YAP
CSB-EP735077YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica 37S ribosomal protein S9, mitochondrial (MRPS9) CSB-YP735078YAP
CSB-EP735078YAP
CSB-BP735078YAP
CSB-MP735078YAP
CSB-EP735078YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Nucleolar protein 58 (NOP58), partial CSB-YP735079YAP
CSB-EP735079YAP
CSB-BP735079YAP
CSB-MP735079YAP
CSB-EP735079YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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