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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xenopus laevis NAD-dependent protein deacylase sirtuin-5B, mitochondrial (sirt5-b) CSB-YP734660XBE
CSB-EP734660XBE
CSB-BP734660XBE
CSB-MP734660XBE
CSB-EP734660XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein unc-45 homolog B (unc45b), partial CSB-YP734661XBE
CSB-EP734661XBE
CSB-BP734661XBE
CSB-MP734661XBE
CSB-EP734661XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis SH3 and cysteine-rich domain-containing protein 3 (stac3) CSB-YP734662XBF
CSB-EP734662XBF
CSB-BP734662XBF
CSB-MP734662XBF
CSB-EP734662XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Folliculin-interacting protein 1 (Fnip1), partial CSB-YP734663MO
CSB-EP734663MO
CSB-BP734663MO
CSB-MP734663MO
CSB-EP734663MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Zinc finger C4H2 domain-containing protein (Zc4h2) CSB-YP734664MO
CSB-EP734664MO
CSB-BP734664MO
CSB-MP734664MO
CSB-EP734664MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis p21-activated protein kinase-interacting protein 1-like (pak1ip1) CSB-YP734665XBE
CSB-EP734665XBE
CSB-BP734665XBE
CSB-MP734665XBE
CSB-EP734665XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Succinate dehydrogenase [ubiquinone] cytochrome b small subunit B, mitochondrial (sdhdb), partial CSB-YP734666DIL1
CSB-EP734666DIL1
CSB-BP734666DIL1
CSB-MP734666DIL1
CSB-EP734666DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Conserved oligomeric Golgi complex subunit 6 (Cog6) CSB-YP734667RA
CSB-EP734667RA
CSB-BP734667RA
CSB-MP734667RA
CSB-EP734667RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1 (Pyroxd1) CSB-YP734668RA
CSB-EP734668RA
CSB-BP734668RA
CSB-MP734668RA
CSB-EP734668RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat ADP-ribosylation factor-like protein 6-interacting protein 6 (Arl6ip6), partial CSB-YP734669RA1
CSB-EP734669RA1
CSB-BP734669RA1
CSB-MP734669RA1
CSB-EP734669RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Cell division cycle-associated protein 3 (Cdca3) CSB-YP734670RA
CSB-EP734670RA
CSB-BP734670RA
CSB-MP734670RA
CSB-EP734670RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat snRNA-activating protein complex subunit 2 (Snapc2) CSB-YP734671RA
CSB-EP734671RA
CSB-BP734671RA
CSB-MP734671RA
CSB-EP734671RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pygathrix roxellana Insulin-like growth factor I (IGF1) CSB-YP734672PGAL
CSB-EP734672PGAL
CSB-BP734672PGAL
CSB-MP734672PGAL
CSB-EP734672PGAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Centruroides exilicauda Toxin Cex8 CSB-YP734673CFAY
CSB-EP734673CFAY
CSB-BP734673CFAY
CSB-MP734673CFAY
CSB-EP734673CFAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Cocaine- and amphetamine-regulated transcript protein (CARTPT) CSB-YP734674BO
CSB-EP734674BO
CSB-BP734674BO
CSB-MP734674BO
CSB-EP734674BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Panax ginseng NAD (P)H-quinone oxidoreductase subunit I, chloroplastic (ndhI) CSB-YP734675EQW
CSB-EP734675EQW
CSB-BP734675EQW
CSB-MP734675EQW
CSB-EP734675EQW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Panax ginseng 50S ribosomal protein L22, chloroplastic (rpl22) CSB-YP734676EQW
CSB-EP734676EQW
CSB-BP734676EQW
CSB-MP734676EQW
CSB-EP734676EQW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Panax ginseng DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP734677EQW
CSB-EP734677EQW
CSB-BP734677EQW
CSB-MP734677EQW
CSB-EP734677EQW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Panax ginseng 50S ribosomal protein L20, chloroplastic (rpl20) CSB-YP734678EQW
CSB-EP734678EQW
CSB-BP734678EQW
CSB-MP734678EQW
CSB-EP734678EQW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Panax ginseng Photosystem II reaction center protein J (psbJ), partial CSB-YP734679EQW
CSB-EP734679EQW
CSB-BP734679EQW
CSB-MP734679EQW
CSB-EP734679EQW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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