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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Burkholderia pseudomallei 4-hydroxy-2-oxovalerate aldolase (mhpE) CSB-YP734018BXW
CSB-EP734018BXW
CSB-BP734018BXW
CSB-MP734018BXW
CSB-EP734018BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei tRNA pseudouridine synthase A (truA) CSB-YP734019BXW
CSB-EP734019BXW
CSB-BP734019BXW
CSB-MP734019BXW
CSB-EP734019BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Translocator protein BipD (bipD) CSB-YP734020BXW
CSB-EP734020BXW
CSB-BP734020BXW
CSB-MP734020BXW
CSB-EP734020BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei UPF0502 protein BPSS1373 (BPSS1373) CSB-YP734021BXW
CSB-EP734021BXW
CSB-BP734021BXW
CSB-MP734021BXW
CSB-EP734021BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Hydroxyethylthiazole kinase (thiM) CSB-YP734022BXW
CSB-EP734022BXW
CSB-BP734022BXW
CSB-MP734022BXW
CSB-EP734022BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei UvrABC system protein B (uvrB), partial CSB-YP734023BXW
CSB-EP734023BXW
CSB-BP734023BXW
CSB-MP734023BXW
CSB-EP734023BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP734024BXW
CSB-EP734024BXW
CSB-BP734024BXW
CSB-MP734024BXW
CSB-EP734024BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei ATP synthase subunit beta 1 (atpD1) CSB-YP734025BXW
CSB-EP734025BXW
CSB-BP734025BXW
CSB-MP734025BXW
CSB-EP734025BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei UPF0102 protein BPSL3274 (BPSL3274) CSB-YP734026BXW
CSB-EP734026BXW
CSB-BP734026BXW
CSB-MP734026BXW
CSB-EP734026BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 50S ribosomal protein L22 (rplV) CSB-YP734027BXW
CSB-EP734027BXW
CSB-BP734027BXW
CSB-MP734027BXW
CSB-EP734027BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 30S ribosomal protein S14 (rpsN) CSB-YP734028BXW
CSB-EP734028BXW
CSB-BP734028BXW
CSB-MP734028BXW
CSB-EP734028BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Translation initiation factor IF-1 2 (infA2) CSB-YP734029BXW
CSB-EP734029BXW
CSB-BP734029BXW
CSB-MP734029BXW
CSB-EP734029BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP734030BXW
CSB-EP734030BXW
CSB-BP734030BXW
CSB-MP734030BXW
CSB-EP734030BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 3-dehydroquinate synthase (aroB) CSB-YP734031BXW
CSB-EP734031BXW
CSB-BP734031BXW
CSB-MP734031BXW
CSB-EP734031BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Histidinol dehydrogenase (hisD) CSB-YP734032BXW
CSB-EP734032BXW
CSB-BP734032BXW
CSB-MP734032BXW
CSB-EP734032BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Sec-independent protein translocase protein TatB (tatB), partial CSB-YP734033BXW
CSB-EP734033BXW
CSB-BP734033BXW
CSB-MP734033BXW
CSB-EP734033BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Cell division protein FtsL (ftsL), partial CSB-YP734034BXW
CSB-EP734034BXW
CSB-BP734034BXW
CSB-MP734034BXW
CSB-EP734034BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Glutamate 5-kinase (proB) CSB-YP734035BXW
CSB-EP734035BXW
CSB-BP734035BXW
CSB-MP734035BXW
CSB-EP734035BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP734036BXW
CSB-EP734036BXW
CSB-BP734036BXW
CSB-MP734036BXW
CSB-EP734036BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Phosphonates import ATP-binding protein PhnC (phnC) CSB-YP734037BXW
CSB-EP734037BXW
CSB-BP734037BXW
CSB-MP734037BXW
CSB-EP734037BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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