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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP734620SRW
CSB-EP734620SRW
CSB-BP734620SRW
CSB-MP734620SRW
CSB-EP734620SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable transcriptional regulatory protein STH1004 (STH1004) CSB-YP734621SRW
CSB-EP734621SRW
CSB-BP734621SRW
CSB-MP734621SRW
CSB-EP734621SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant D-ribose pyranase (rbsD) CSB-YP734622SRW
CSB-EP734622SRW
CSB-BP734622SRW
CSB-MP734622SRW
CSB-EP734622SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein CrcB homolog 2 (crcB2), partial CSB-YP734623SRW1
CSB-EP734623SRW1
CSB-BP734623SRW1
CSB-MP734623SRW1
CSB-EP734623SRW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein DnaJ (dnaJ) CSB-YP734624SRW
CSB-EP734624SRW
CSB-BP734624SRW
CSB-MP734624SRW
CSB-EP734624SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GTPase obg (obg) CSB-YP734625SRW
CSB-EP734625SRW
CSB-BP734625SRW
CSB-MP734625SRW
CSB-EP734625SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maf-like protein STH370 (STH370) CSB-YP734626SRW
CSB-EP734626SRW
CSB-BP734626SRW
CSB-MP734626SRW
CSB-EP734626SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate racemase (murI) CSB-YP734627SRW
CSB-EP734627SRW
CSB-BP734627SRW
CSB-MP734627SRW
CSB-EP734627SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoglycerate kinase (pgk) CSB-YP734628SRW
CSB-EP734628SRW
CSB-BP734628SRW
CSB-MP734628SRW
CSB-EP734628SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent zinc metalloprotease FtsH 2 (ftsH2), partial CSB-YP734629SRW
CSB-EP734629SRW
CSB-BP734629SRW
CSB-MP734629SRW
CSB-EP734629SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit beta (atpD) CSB-YP734630SRW
CSB-EP734630SRW
CSB-BP734630SRW
CSB-MP734630SRW
CSB-EP734630SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide chain release factor 1 (prfA) CSB-YP734631SRW
CSB-EP734631SRW
CSB-BP734631SRW
CSB-MP734631SRW
CSB-EP734631SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA replication and repair protein recF (recF) CSB-YP734632SRW
CSB-EP734632SRW
CSB-BP734632SRW
CSB-MP734632SRW
CSB-EP734632SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Membrane-anchored ubiquitin-fold protein 2 (MUB2) CSB-YP734633OFG
CSB-EP734633OFG
CSB-BP734633OFG
CSB-MP734633OFG
CSB-EP734633OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Cyclin-D1-1 (CYCD1-1) CSB-YP734634OFG
CSB-EP734634OFG
CSB-BP734634OFG
CSB-MP734634OFG
CSB-EP734634OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Transcription initiation factor TFIID subunit 1 (TAF1), partial CSB-YP734635OFG
CSB-EP734635OFG
CSB-BP734635OFG
CSB-MP734635OFG
CSB-EP734635OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable mannan synthase 3 (CSLA3), partial CSB-YP734636OFG1
CSB-EP734636OFG1
CSB-BP734636OFG1
CSB-MP734636OFG1
CSB-EP734636OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein LURP-one-related 3 (At1g53890) CSB-YP734637DOA
CSB-EP734637DOA
CSB-BP734637DOA
CSB-MP734637DOA
CSB-EP734637DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized protein At4g14100 (At4g14100) CSB-YP734638DOA
CSB-EP734638DOA
CSB-BP734638DOA
CSB-MP734638DOA
CSB-EP734638DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cytokinin dehydrogenase 5 (CKX5), partial CSB-YP734639DOA
CSB-EP734639DOA
CSB-BP734639DOA
CSB-MP734639DOA
CSB-EP734639DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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