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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant 50S ribosomal protein L1 (rplA) CSB-YP734499YAH
CSB-EP734499YAH
CSB-BP734499YAH
CSB-MP734499YAH
CSB-EP734499YAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable ubiquinone biosynthesis protein UbiB (ubiB), partial CSB-YP734500YAH1
CSB-EP734500YAH1
CSB-BP734500YAH1
CSB-MP734500YAH1
CSB-EP734500YAH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant sn-glycerol-3-phosphate transport system permease protein ugpE (ugpE), partial CSB-YP734501YAH1
CSB-EP734501YAH1
CSB-BP734501YAH1
CSB-MP734501YAH1
CSB-EP734501YAH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphobilinogen deaminase (hemC) CSB-YP734502YAH
CSB-EP734502YAH
CSB-BP734502YAH
CSB-MP734502YAH
CSB-EP734502YAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant HTH-type transcriptional regulator hdfR (hdfR) CSB-YP734503YAH
CSB-EP734503YAH
CSB-BP734503YAH
CSB-MP734503YAH
CSB-EP734503YAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP734504YAH
CSB-EP734504YAH
CSB-BP734504YAH
CSB-MP734504YAH
CSB-EP734504YAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L31 (rpmE) CSB-YP734505YAH
CSB-EP734505YAH
CSB-BP734505YAH
CSB-MP734505YAH
CSB-EP734505YAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cation-efflux pump FieF (fieF), partial CSB-YP734506YAH1
CSB-EP734506YAH1
CSB-BP734506YAH1
CSB-MP734506YAH1
CSB-EP734506YAH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L33 (rpmG) CSB-YP734507YAH
CSB-EP734507YAH
CSB-BP734507YAH
CSB-MP734507YAH
CSB-EP734507YAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guanylate kinase (gmk) CSB-YP734508YAH
CSB-EP734508YAH
CSB-BP734508YAH
CSB-MP734508YAH
CSB-EP734508YAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana CASP-like protein At4g37235 (At4g37235), partial CSB-YP734509DOA1
CSB-EP734509DOA1
CSB-BP734509DOA1
CSB-MP734509DOA1
CSB-EP734509DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At5g13770, chloroplastic (At5g13770), partial CSB-YP734510DOA
CSB-EP734510DOA
CSB-BP734510DOA
CSB-MP734510DOA
CSB-EP734510DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable beta-1,3-galactosyltransferase 12 (B3GALT12), partial CSB-YP734511DOA1
CSB-EP734511DOA1
CSB-BP734511DOA1
CSB-MP734511DOA1
CSB-EP734511DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Probable G-protein coupled receptor 160 (Gpr160), partial CSB-YP734512RA1
CSB-EP734512RA1
CSB-BP734512RA1
CSB-MP734512RA1
CSB-EP734512RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Uncharacterized protein C17orf59 homolog CSB-YP734513RA
CSB-EP734513RA
CSB-BP734513RA
CSB-MP734513RA
CSB-EP734513RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat 39S ribosomal protein L24, mitochondrial (Mrpl24) CSB-YP734514RA
CSB-EP734514RA
CSB-BP734514RA
CSB-MP734514RA
CSB-EP734514RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat NEDD4-binding protein 2-like 2 (N4bp2l2) CSB-YP734515RA
CSB-EP734515RA
CSB-BP734515RA
CSB-MP734515RA
CSB-EP734515RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Coiled-coil domain-containing protein 82 (Ccdc82) CSB-YP734516RA
CSB-EP734516RA
CSB-BP734516RA
CSB-MP734516RA
CSB-EP734516RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Coatomer subunit delta (Arcn1) CSB-YP734517RA
CSB-EP734517RA
CSB-BP734517RA
CSB-MP734517RA
CSB-EP734517RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Coiled-coil domain-containing protein 41 (Ccdc41) CSB-YP734518RA
CSB-EP734518RA
CSB-BP734518RA
CSB-MP734518RA
CSB-EP734518RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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