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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Kluyveromyces lactis Calpain-like protease palB/RIM13 (RIM13), partial CSB-YP735100KBK
CSB-EP735100KBK
CSB-BP735100KBK
CSB-MP735100KBK
CSB-EP735100KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Inheritance of peroxisomes protein 1 (INP1) CSB-YP735101KBK
CSB-EP735101KBK
CSB-BP735101KBK
CSB-MP735101KBK
CSB-EP735101KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Conserved oligomeric Golgi complex subunit 6 (COG6), partial CSB-YP735102KBK
CSB-EP735102KBK
CSB-BP735102KBK
CSB-MP735102KBK
CSB-EP735102KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Flap endonuclease 1 (FEN1) CSB-YP735103KBK
CSB-EP735103KBK
CSB-BP735103KBK
CSB-MP735103KBK
CSB-EP735103KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Cytochrome oxidase assembly protein 3, mitochondrial (COA3), partial CSB-YP735104KBK1
CSB-EP735104KBK1
CSB-BP735104KBK1
CSB-MP735104KBK1
CSB-EP735104KBK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Serine hydroxymethyltransferase, mitochondrial (SHM1) CSB-YP735105KBK
CSB-EP735105KBK
CSB-BP735105KBK
CSB-MP735105KBK
CSB-EP735105KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Altered inheritance of mitochondria protein 43, mitochondrial (AIM43), partial CSB-YP735106KBK1
CSB-EP735106KBK1
CSB-BP735106KBK1
CSB-MP735106KBK1
CSB-EP735106KBK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Mediator of RNA polymerase II transcription subunit 8 (MED8) CSB-YP735107KBK
CSB-EP735107KBK
CSB-BP735107KBK
CSB-MP735107KBK
CSB-EP735107KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Enolase-phosphatase E1 (UTR4) CSB-YP735108KBK
CSB-EP735108KBK
CSB-BP735108KBK
CSB-MP735108KBK
CSB-EP735108KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Protein SBE2 (SBE2), partial CSB-YP735109KBK
CSB-EP735109KBK
CSB-BP735109KBK
CSB-MP735109KBK
CSB-EP735109KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Glutamyl-tRNA (Gln) amidotransferase subunit B, mitochondrial (PET112) CSB-YP735110KBK
CSB-EP735110KBK
CSB-BP735110KBK
CSB-MP735110KBK
CSB-EP735110KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Probable endonuclease LCL3 (LCL3), partial CSB-YP735111KBK1
CSB-EP735111KBK1
CSB-BP735111KBK1
CSB-MP735111KBK1
CSB-EP735111KBK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis FAS1 domain-containing protein KLLA0E16841g (KLLA0E16841g) CSB-YP735112KBK
CSB-EP735112KBK
CSB-BP735112KBK
CSB-MP735112KBK
CSB-EP735112KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Topoisomerase I damage affected protein 11 (TDA11) CSB-YP735113KBK
CSB-EP735113KBK
CSB-BP735113KBK
CSB-MP735113KBK
CSB-EP735113KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis ATP-dependent rRNA helicase SPB4 (SPB4), partial CSB-YP735114KBK
CSB-EP735114KBK
CSB-BP735114KBK
CSB-MP735114KBK
CSB-EP735114KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Chromatin modification-related protein EAF3 (EAF3) CSB-YP735115KBK
CSB-EP735115KBK
CSB-BP735115KBK
CSB-MP735115KBK
CSB-EP735115KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Mitotic spindle-associated protein SHE1 (SHE1) CSB-YP735116KBK
CSB-EP735116KBK
CSB-BP735116KBK
CSB-MP735116KBK
CSB-EP735116KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis rRNA-processing protein CGR1 (CGR1) CSB-YP735117KBK
CSB-EP735117KBK
CSB-BP735117KBK
CSB-MP735117KBK
CSB-EP735117KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Sulfate adenylyltransferase (MET3), partial CSB-YP735118KBK
CSB-EP735118KBK
CSB-BP735118KBK
CSB-MP735118KBK
CSB-EP735118KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis 37S ribosomal protein S25, mitochondrial (RSM25) CSB-YP735119KBK
CSB-EP735119KBK
CSB-BP735119KBK
CSB-MP735119KBK
CSB-EP735119KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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