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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Thermus thermophilus 3-dehydroquinate dehydratase (aroQ) CSB-YP735918TNT
CSB-EP735918TNT
CSB-BP735918TNT
CSB-MP735918TNT
CSB-EP735918TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Acetyl-coenzyme A synthetase (acsA), partial CSB-YP735919TNT
CSB-EP735919TNT
CSB-BP735919TNT
CSB-MP735919TNT
CSB-EP735919TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Maf-like protein TTHA1188 (TTHA1188) CSB-YP735920TNT
CSB-EP735920TNT
CSB-BP735920TNT
CSB-MP735920TNT
CSB-EP735920TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus N utilization substance protein B homolog (nusB) CSB-YP735921TNT
CSB-EP735921TNT
CSB-BP735921TNT
CSB-MP735921TNT
CSB-EP735921TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Tryptophan synthase beta chain (trpB) CSB-YP735922TNT
CSB-EP735922TNT
CSB-BP735922TNT
CSB-MP735922TNT
CSB-EP735922TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP735923TNT
CSB-EP735923TNT
CSB-BP735923TNT
CSB-MP735923TNT
CSB-EP735923TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus UPF0365 protein TTHA1048 (TTHA1048), partial CSB-YP735924TNT1
CSB-EP735924TNT1
CSB-BP735924TNT1
CSB-MP735924TNT1
CSB-EP735924TNT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 4-hydroxyphenylacetate 3-monooxygenase reductase component (TTHA0961) CSB-YP735925TNT
CSB-EP735925TNT
CSB-BP735925TNT
CSB-MP735925TNT
CSB-EP735925TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Triosephosphate isomerase (tpiA) CSB-YP735926TNT
CSB-EP735926TNT
CSB-BP735926TNT
CSB-MP735926TNT
CSB-EP735926TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Dihydroorotate dehydrogenase (quinone) (pyrD) CSB-YP735927TNT
CSB-EP735927TNT
CSB-BP735927TNT
CSB-MP735927TNT
CSB-EP735927TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Protein translocase subunit SecDF (secDF), partial CSB-YP735928TNT
CSB-EP735928TNT
CSB-BP735928TNT
CSB-MP735928TNT
CSB-EP735928TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP735929TNT
CSB-EP735929TNT
CSB-BP735929TNT
CSB-MP735929TNT
CSB-EP735929TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Biotin synthase (bioB) CSB-YP735930TNT
CSB-EP735930TNT
CSB-BP735930TNT
CSB-MP735930TNT
CSB-EP735930TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 50S ribosomal protein L35 (rpmI) CSB-YP735931TNT
CSB-EP735931TNT
CSB-BP735931TNT
CSB-MP735931TNT
CSB-EP735931TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP735932TNT
CSB-EP735932TNT
CSB-BP735932TNT
CSB-MP735932TNT
CSB-EP735932TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus UPF0102 protein TTHA0372 (TTHA0372) CSB-YP735933TNT
CSB-EP735933TNT
CSB-BP735933TNT
CSB-MP735933TNT
CSB-EP735933TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Peptide deformylase (def) CSB-YP735934TNT
CSB-EP735934TNT
CSB-BP735934TNT
CSB-MP735934TNT
CSB-EP735934TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Protoheme IX farnesyltransferase (ctaB), partial CSB-YP735935TNT
CSB-EP735935TNT
CSB-BP735935TNT
CSB-MP735935TNT
CSB-EP735935TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 50S ribosomal protein L11 (rplK) CSB-YP735936TNT
CSB-EP735936TNT
CSB-BP735936TNT
CSB-MP735936TNT
CSB-EP735936TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 30S ribosomal protein S18 (rpsR) CSB-YP735937TNT
CSB-EP735937TNT
CSB-BP735937TNT
CSB-MP735937TNT
CSB-EP735937TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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