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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Debaryomyces hansenii pH-response regulator protein palH/RIM21 (RIM21), partial CSB-YP734980DIS1
CSB-EP734980DIS1
CSB-BP734980DIS1
CSB-MP734980DIS1
CSB-EP734980DIS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Probable kinetochore protein NDC80 (NDC80), partial CSB-YP734981DIS
CSB-EP734981DIS
CSB-BP734981DIS
CSB-MP734981DIS
CSB-EP734981DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii ATP-dependent rRNA helicase RRP3 (RRP3) CSB-YP734982DIS
CSB-EP734982DIS
CSB-BP734982DIS
CSB-MP734982DIS
CSB-EP734982DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Golgi apparatus membrane protein TVP38 (TVP38), partial CSB-YP734983DIS1
CSB-EP734983DIS1
CSB-BP734983DIS1
CSB-MP734983DIS1
CSB-EP734983DIS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Polynucleotide 5'-hydroxyl-kinase GRC3 (GRC3), partial CSB-YP734984DIS
CSB-EP734984DIS
CSB-BP734984DIS
CSB-MP734984DIS
CSB-EP734984DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii 60S ribosomal subunit assembly/export protein LOC1 (LOC1) CSB-YP734985DIS
CSB-EP734985DIS
CSB-BP734985DIS
CSB-MP734985DIS
CSB-EP734985DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Ribosome biogenesis protein ERB1 (ERB1), partial CSB-YP734986DIS
CSB-EP734986DIS
CSB-BP734986DIS
CSB-MP734986DIS
CSB-EP734986DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Protein transport protein SEC31 (SEC31), partial CSB-YP734987DIS
CSB-EP734987DIS
CSB-BP734987DIS
CSB-MP734987DIS
CSB-EP734987DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Serine/threonine-protein kinase ATG1 (ATG1), partial CSB-YP734988DIS
CSB-EP734988DIS
CSB-BP734988DIS
CSB-MP734988DIS
CSB-EP734988DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii V-type proton ATPase 16 kDa proteolipid subunit 2 (VMA11), partial CSB-YP734989DIS1
CSB-EP734989DIS1
CSB-BP734989DIS1
CSB-MP734989DIS1
CSB-EP734989DIS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Vacuolar-sorting protein SNF7 (SNF7) CSB-YP734990DIS
CSB-EP734990DIS
CSB-BP734990DIS
CSB-MP734990DIS
CSB-EP734990DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii ATPase GET3 (GET3) CSB-YP734991DIS
CSB-EP734991DIS
CSB-BP734991DIS
CSB-MP734991DIS
CSB-EP734991DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Protein SSH4 (SSH4), partial CSB-YP734992DIS
CSB-EP734992DIS
CSB-BP734992DIS
CSB-MP734992DIS
CSB-EP734992DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Eukaryotic translation initiation factor 3 subunit G (TIF35) CSB-YP734993DIS
CSB-EP734993DIS
CSB-BP734993DIS
CSB-MP734993DIS
CSB-EP734993DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Exocyst complex protein EXO70 (EXO70), partial CSB-YP734994DIS
CSB-EP734994DIS
CSB-BP734994DIS
CSB-MP734994DIS
CSB-EP734994DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Histone-lysine N-methyltransferase, H3 lysine-79 specific (DOT1), partial CSB-YP734995DIS
CSB-EP734995DIS
CSB-BP734995DIS
CSB-MP734995DIS
CSB-EP734995DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Autophagy-related protein 2 (ATG2), partial CSB-YP734996DIS
CSB-EP734996DIS
CSB-BP734996DIS
CSB-MP734996DIS
CSB-EP734996DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Pre-mRNA-splicing factor ISY1 (ISY1) CSB-YP734997DIS
CSB-EP734997DIS
CSB-BP734997DIS
CSB-MP734997DIS
CSB-EP734997DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Protein CLP1 (CLP1) CSB-YP734998DIS
CSB-EP734998DIS
CSB-BP734998DIS
CSB-MP734998DIS
CSB-EP734998DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Mediator of RNA polymerase II transcription subunit 31 (SOH1) CSB-YP734999DIS
CSB-EP734999DIS
CSB-BP734999DIS
CSB-MP734999DIS
CSB-EP734999DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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