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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Thermus thermophilus ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP735938TNT
CSB-EP735938TNT
CSB-BP735938TNT
CSB-MP735938TNT
CSB-EP735938TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP735939TNT
CSB-EP735939TNT
CSB-BP735939TNT
CSB-MP735939TNT
CSB-EP735939TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica 60S ribosomal protein L39-3 (RPL39C) CSB-YP735940OFG
CSB-EP735940OFG
CSB-BP735940OFG
CSB-MP735940OFG
CSB-EP735940OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable protein phosphatase 2C 8 (Os01g0656200, LOC_Os01g46760) CSB-YP735941OFG
CSB-EP735941OFG
CSB-BP735941OFG
CSB-MP735941OFG
CSB-EP735941OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Zinc finger CCCH domain-containing protein 40 (Os06g0170500, LOC_Os06g07350) CSB-YP735942OFG
CSB-EP735942OFG
CSB-BP735942OFG
CSB-MP735942OFG
CSB-EP735942OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Glucoside xylosyltransferase 1 (gxylt1), partial CSB-YP735943DIL
CSB-EP735943DIL
CSB-BP735943DIL
CSB-MP735943DIL
CSB-EP735943DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Cation transport regulator-like protein 1 (chac1) CSB-YP735944DIL
CSB-EP735944DIL
CSB-BP735944DIL
CSB-MP735944DIL
CSB-EP735944DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative uncharacterized protein C9orf141 (C9orf141) CSB-YP735945HU
CSB-EP735945HU
CSB-BP735945HU
CSB-MP735945HU
CSB-EP735945HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Cytoplasmic FMR1-interacting protein 2 (Cyfip2), partial CSB-YP735946MO
CSB-EP735946MO
CSB-BP735946MO
CSB-MP735946MO
CSB-EP735946MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Nucleoporin NUP188 homolog (NUP188), partial CSB-YP735947HU
CSB-EP735947HU
CSB-BP735947HU
CSB-MP735947HU
CSB-EP735947HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Dehydrogenase/reductase SDR family member 13 (Dhrs13) CSB-YP735948MO
CSB-EP735948MO
CSB-BP735948MO
CSB-MP735948MO
CSB-EP735948MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein FAM46C (Fam46c) CSB-YP735949MO
CSB-EP735949MO
CSB-BP735949MO
CSB-MP735949MO
CSB-EP735949MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Neuferricin (Cyb5d2) CSB-YP735950MO
CSB-EP735950MO
CSB-BP735950MO
CSB-MP735950MO
CSB-EP735950MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Valine--tRNA ligase, mitochondrial (VARS2), partial CSB-YP735951HU
CSB-EP735951HU
CSB-BP735951HU
CSB-MP735951HU
CSB-EP735951HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ankyrin repeat domain-containing protein 40 (Ankrd40) CSB-YP735952MO
CSB-EP735952MO
CSB-BP735952MO
CSB-MP735952MO
CSB-EP735952MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Spermatogenesis-associated protein 22 (Spata22) CSB-YP735953MO
CSB-EP735953MO
CSB-BP735953MO
CSB-MP735953MO
CSB-EP735953MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Leukocyte elastase inhibitor C (Serpinb1c) CSB-YP735954MO
CSB-EP735954MO
CSB-BP735954MO
CSB-MP735954MO
CSB-EP735954MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human PGC-1 and ERR-induced regulator in muscle protein 1 (PERM1), partial CSB-YP735955HU
CSB-EP735955HU
CSB-BP735955HU
CSB-MP735955HU
CSB-EP735955HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Clustered mitochondria protein homolog (Cluh), partial CSB-YP735956MO
CSB-EP735956MO
CSB-BP735956MO
CSB-MP735956MO
CSB-EP735956MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Myosin-4 (Myh4), partial CSB-YP735957MO
CSB-EP735957MO
CSB-BP735957MO
CSB-MP735957MO
CSB-EP735957MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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