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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xenopus tropicalis GPI mannosyltransferase 1 (pigm), partial CSB-YP737752XBF
CSB-EP737752XBF
CSB-BP737752XBF
CSB-MP737752XBF
CSB-EP737752XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Zona pellucida-like domain-containing protein 1 (zpld1), partial CSB-YP737753XBE
CSB-EP737753XBE
CSB-BP737753XBE
CSB-MP737753XBE
CSB-EP737753XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Immediate early response gene 5-like protein (ier5l) CSB-YP737754XBE
CSB-EP737754XBE
CSB-BP737754XBE
CSB-MP737754XBE
CSB-EP737754XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Interferon regulatory factor 2-binding protein-like A (irf2bpl-a), partial CSB-YP737755XBE
CSB-EP737755XBE
CSB-BP737755XBE
CSB-MP737755XBE
CSB-EP737755XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis UPF0694 transmembrane protein C14orf109 homolog A, partial CSB-YP737756XBE1
CSB-EP737756XBE1
CSB-BP737756XBE1
CSB-MP737756XBE1
CSB-EP737756XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Nuclear factor interleukin-3-regulated protein (nfil3) CSB-YP737757XBE
CSB-EP737757XBE
CSB-BP737757XBE
CSB-MP737757XBE
CSB-EP737757XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Forkhead box protein N2 (foxn2) CSB-YP737758XBE
CSB-EP737758XBE
CSB-BP737758XBE
CSB-MP737758XBE
CSB-EP737758XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Histone-lysine N-methyltransferase SETD1B (setd1b), partial CSB-YP737759XBE
CSB-EP737759XBE
CSB-BP737759XBE
CSB-MP737759XBE
CSB-EP737759XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis 45 kDa calcium-binding protein (sdf4) CSB-YP737760XBE
CSB-EP737760XBE
CSB-BP737760XBE
CSB-MP737760XBE
CSB-EP737760XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Translationally-controlled tumor protein homolog (tpt1) CSB-YP737761XBF
CSB-EP737761XBF
CSB-BP737761XBF
CSB-MP737761XBF
CSB-EP737761XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Transcription factor Sox-11 (sox11) CSB-YP737762XBF
CSB-EP737762XBF
CSB-BP737762XBF
CSB-MP737762XBF
CSB-EP737762XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Sprouty-related, EVH1 domain-containing protein 1 (spred1) CSB-YP737763XBF
CSB-EP737763XBF
CSB-BP737763XBF
CSB-MP737763XBF
CSB-EP737763XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Polyribonucleotide 5'-hydroxyl-kinase Clp1 (clp1) CSB-YP737764XBF
CSB-EP737764XBF
CSB-BP737764XBF
CSB-MP737764XBF
CSB-EP737764XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Uncharacterized protein C8orf58 homolog CSB-YP737765MO
CSB-EP737765MO
CSB-BP737765MO
CSB-MP737765MO
CSB-EP737765MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Mannose-1-phosphate guanyltransferase alpha-B (gmppa-b) CSB-YP737766XBE
CSB-EP737766XBE
CSB-BP737766XBE
CSB-MP737766XBE
CSB-EP737766XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Proline-rich nuclear receptor coactivator 2 A (pnrc2-a) CSB-YP737767XBE
CSB-EP737767XBE
CSB-BP737767XBE
CSB-MP737767XBE
CSB-EP737767XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Deoxyhypusine hydroxylase (dohh) CSB-YP737768XBE
CSB-EP737768XBE
CSB-BP737768XBE
CSB-MP737768XBE
CSB-EP737768XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis 39S ribosomal protein L51, mitochondrial (mrpl51) CSB-YP737769XBE
CSB-EP737769XBE
CSB-BP737769XBE
CSB-MP737769XBE
CSB-EP737769XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Calcium-binding mitochondrial carrier protein SCaMC-1 (slc25a24), partial CSB-YP737770DIL1
CSB-EP737770DIL1
CSB-BP737770DIL1
CSB-MP737770DIL1
CSB-EP737770DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fragaria ananassa Putative UDP-rhamnose:rhamnosyltransferase 1 (GT4), partial CSB-YP737771FEZ1
CSB-EP737771FEZ1
CSB-BP737771FEZ1
CSB-MP737771FEZ1
CSB-EP737771FEZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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