重組蛋白
重組蛋白質是分析生物相互作用的有用工具。在研究實驗室中使用了大量的重組蛋白質,包括商業(yè)蛋白質和在特定研究項目中生成的蛋白質。它們在許多應用中發(fā)揮著關鍵作用。
如果您正在尋找具有優(yōu)良質量(驗證的生物活性,較低的內毒素水平和高純度)的重組蛋白質來支持您的研究,您來對地方了。
經(jīng)過16多年的發(fā)展,華美生物已經(jīng)建立了從原核到真核的五大系統(tǒng),用于表達重組蛋白質。我們擁有一系列蛋白質表達和純化技術,包括基于桿狀病毒外膜蛋白(OMP)的蛋白質表達技術,以及膜蛋白表達技術等。
產(chǎn)品特點
- 超過10,000種重組蛋白覆蓋大多數(shù)基因/靶點;
- 5種表達系統(tǒng),包括大腸桿菌(E.coli)、酵母、桿狀病毒感染的昆蟲細胞、哺乳動物細胞和體外大腸桿菌。而體外大腸桿菌表達系統(tǒng)是華美生物的獨特表達系統(tǒng)。與傳統(tǒng)蛋白表達系統(tǒng)相比,體外大腸桿菌省略了許多過程,如質粒轉化、細胞培養(yǎng)、收集、破碎和離心等,大大提高了工作效率。
- 多種標簽,包括His、GST、Flag、MBP等。
- 廣泛應用,包括抗原、細胞分析、結合分析/蛋白質相互作用、細胞培養(yǎng)-無血清培養(yǎng)基、藥物相關研究、體外酶活性、ELISA標準及其原料、體內研究、質譜標準、蛋白芯片、SDS-PAGE控制、蛋白質結構分析(晶體/電子顯微鏡)。
- 多種物種,包括人類、小鼠、大鼠、兔子、豚鼠、雞、猴子、豬等。
- 無動物副產(chǎn)品的動物源性重組蛋白質。華美生物已開發(fā)出無動物副產(chǎn)品接觸的重組蛋白質產(chǎn)品線。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質,或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的抗體,請查看華美生物蛋白質定制服務。
通過選擇目標名稱的首字母瀏覽產(chǎn)品。
產(chǎn)品名稱 | 貨號 | 規(guī)格 |
---|---|---|
Recombinant Haloarcula marismortui Signal recognition 54 kDa protein (srp54) | CSB-YP736158HTJ CSB-EP736158HTJ CSB-BP736158HTJ CSB-MP736158HTJ CSB-EP736158HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui UPF0447 protein rrnAC3100 (rrnAC3100), partial | CSB-YP736159HTJ CSB-EP736159HTJ CSB-BP736159HTJ CSB-MP736159HTJ CSB-EP736159HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui UvrABC system protein C (uvrC), partial | CSB-YP736160HTJ CSB-EP736160HTJ CSB-BP736160HTJ CSB-MP736160HTJ CSB-EP736160HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui Putative ski2-type helicase (rrnAC2876), partial | CSB-YP736161HTJ CSB-EP736161HTJ CSB-BP736161HTJ CSB-MP736161HTJ CSB-EP736161HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui Cell division control protein 6 homolog 4 (cdc6-4), partial | CSB-YP736162HTJ CSB-EP736162HTJ CSB-BP736162HTJ CSB-MP736162HTJ CSB-EP736162HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui Ferredoxin-2 (fer2) | CSB-YP736163HTJ CSB-EP736163HTJ CSB-BP736163HTJ CSB-MP736163HTJ CSB-EP736163HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui Replication factor C small subunit (rfcS) | CSB-YP736164HTJ CSB-EP736164HTJ CSB-BP736164HTJ CSB-MP736164HTJ CSB-EP736164HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui Ribonuclease Z (rnz) | CSB-YP736165HTJ CSB-EP736165HTJ CSB-BP736165HTJ CSB-MP736165HTJ CSB-EP736165HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui CCA-adding enzyme (cca) | CSB-YP736166HTJ CSB-EP736166HTJ CSB-BP736166HTJ CSB-MP736166HTJ CSB-EP736166HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui Phosphate import ATP-binding protein PstB 2 (pstB2) | CSB-YP736167HTJ CSB-EP736167HTJ CSB-BP736167HTJ CSB-MP736167HTJ CSB-EP736167HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui Nicotinamide-nucleotide adenylyltransferase (rrnAC1913) | CSB-YP736168HTJ CSB-EP736168HTJ CSB-BP736168HTJ CSB-MP736168HTJ CSB-EP736168HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui Probable deoxycytidine triphosphate deaminase (dcd) | CSB-YP736169HTJ CSB-EP736169HTJ CSB-BP736169HTJ CSB-MP736169HTJ CSB-EP736169HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui Replication factor C large subunit (rfcL), partial | CSB-YP736170HTJ CSB-EP736170HTJ CSB-BP736170HTJ CSB-MP736170HTJ CSB-EP736170HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui Tritrans,polycis-undecaprenyl-diphosphate synthase (GGDP specific) (uppS) | CSB-YP736171HTJ CSB-EP736171HTJ CSB-BP736171HTJ CSB-MP736171HTJ CSB-EP736171HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui DNA-directed RNA polymerase subunit P (rpoP) | CSB-YP736172HTJ CSB-EP736172HTJ CSB-BP736172HTJ CSB-MP736172HTJ CSB-EP736172HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui Orotidine 5'-phosphate decarboxylase (pyrF) | CSB-YP736173HTJ CSB-EP736173HTJ CSB-BP736173HTJ CSB-MP736173HTJ CSB-EP736173HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui Adenylosuccinate synthetase (purA) | CSB-YP736174HTJ CSB-EP736174HTJ CSB-BP736174HTJ CSB-MP736174HTJ CSB-EP736174HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui Probable cobyrinic acid A,C-diamide synthase (cobB) | CSB-YP736175HTJ CSB-EP736175HTJ CSB-BP736175HTJ CSB-MP736175HTJ CSB-EP736175HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) | CSB-YP736176HTJ CSB-EP736176HTJ CSB-BP736176HTJ CSB-MP736176HTJ CSB-EP736176HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial | CSB-YP736177HTJ CSB-EP736177HTJ CSB-BP736177HTJ CSB-MP736177HTJ CSB-EP736177HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。
第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條:如何選擇合適的表達載體?
表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。
第三條:如何表達具有生物活性的蛋白質?
根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022