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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xenopus laevis Ammonium transporter Rh type B-A (rhbg-a), partial CSB-YP737973XBE
CSB-EP737973XBE
CSB-BP737973XBE
CSB-MP737973XBE
CSB-EP737973XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichophyton rubrum Subtilisin-like protease 1 (SUB1) CSB-YP737974TQA
CSB-EP737974TQA
CSB-BP737974TQA
CSB-MP737974TQA
CSB-EP737974TQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromosome partition protein Smc (smc), partial CSB-YP737975MSD
CSB-EP737975MSD
CSB-BP737975MSD
CSB-MP737975MSD
CSB-EP737975MSD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Potassium transporter 22 (HAK22), partial CSB-YP737976OFG
CSB-EP737976OFG
CSB-BP737976OFG
CSB-MP737976OFG
CSB-EP737976OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable 6-phosphogluconolactonase 4, chloroplastic (Os09g0529100, LOC_Os09g35970) CSB-YP737977OFG
CSB-EP737977OFG
CSB-BP737977OFG
CSB-MP737977OFG
CSB-EP737977OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Cyclin-D3-1 (CYCD3-1) CSB-YP737978OFG
CSB-EP737978OFG
CSB-BP737978OFG
CSB-MP737978OFG
CSB-EP737978OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable potassium transporter 14 (HAK14), partial CSB-YP737979OFG
CSB-EP737979OFG
CSB-BP737979OFG
CSB-MP737979OFG
CSB-EP737979OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Ammonium transporter 3 member 3 (AMT3-3), partial CSB-YP737980OFG
CSB-EP737980OFG
CSB-BP737980OFG
CSB-MP737980OFG
CSB-EP737980OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Putative 12-oxophytodienoate reductase 5 (OPR5) CSB-YP737981OFG
CSB-EP737981OFG
CSB-BP737981OFG
CSB-MP737981OFG
CSB-EP737981OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica 40S ribosomal protein S13-2 (Os08g0117300, LOC_Os08g02410) CSB-YP737982OFG
CSB-EP737982OFG
CSB-BP737982OFG
CSB-MP737982OFG
CSB-EP737982OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Auxin-responsive protein IAA23 (IAA23) CSB-YP737983OFG
CSB-EP737983OFG
CSB-BP737983OFG
CSB-MP737983OFG
CSB-EP737983OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Zinc finger CCCH domain-containing protein 44 (Os06g0618100, LOC_Os06g41384) CSB-YP737984OFG
CSB-EP737984OFG
CSB-BP737984OFG
CSB-MP737984OFG
CSB-EP737984OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Uncharacterized protein NCBP2-AS2 (NCBP2-AS2) CSB-YP737985HU
CSB-EP737985HU
CSB-BP737985HU
CSB-MP737985HU
CSB-EP737985HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Pseudopodium-enriched atypical kinase 1 (Peak1), partial CSB-YP737986MO
CSB-EP737986MO
CSB-BP737986MO
CSB-MP737986MO
CSB-EP737986MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Leucine-rich repeat and coiled-coil domain-containing protein 1 (Lrrcc1), partial CSB-YP737987MO
CSB-EP737987MO
CSB-BP737987MO
CSB-MP737987MO
CSB-EP737987MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Rho GTPase-activating protein 23 (Arhgap23), partial CSB-YP737988MO
CSB-EP737988MO
CSB-BP737988MO
CSB-MP737988MO
CSB-EP737988MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Serine/threonine-protein kinase 36 (Stk36), partial CSB-YP737989MO
CSB-EP737989MO
CSB-BP737989MO
CSB-MP737989MO
CSB-EP737989MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse E3 ubiquitin-protein ligase PDZRN3 (Pdzrn3), partial CSB-YP737990MO
CSB-EP737990MO
CSB-BP737990MO
CSB-MP737990MO
CSB-EP737990MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse EH domain-binding protein 1 (Ehbp1), partial CSB-YP737991MO
CSB-EP737991MO
CSB-BP737991MO
CSB-MP737991MO
CSB-EP737991MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Switch-associated protein 70 (Swap70) CSB-YP737992MO
CSB-EP737992MO
CSB-BP737992MO
CSB-MP737992MO
CSB-EP737992MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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