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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Adenylosuccinate synthetase (purA) CSB-YP736419SMZ
CSB-EP736419SMZ
CSB-BP736419SMZ
CSB-MP736419SMZ
CSB-EP736419SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP736420SMZ
CSB-EP736420SMZ
CSB-BP736420SMZ
CSB-MP736420SMZ
CSB-EP736420SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Translation initiation factor IF-1 (infA) CSB-YP736421SMZ
CSB-EP736421SMZ
CSB-BP736421SMZ
CSB-MP736421SMZ
CSB-EP736421SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L24 (rplX) CSB-YP736422SMZ
CSB-EP736422SMZ
CSB-BP736422SMZ
CSB-MP736422SMZ
CSB-EP736422SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S19 (rpsS) CSB-YP736423SMZ
CSB-EP736423SMZ
CSB-BP736423SMZ
CSB-MP736423SMZ
CSB-EP736423SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphate acyltransferase (plsX) CSB-YP736424SMZ
CSB-EP736424SMZ
CSB-BP736424SMZ
CSB-MP736424SMZ
CSB-EP736424SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromosomal replication initiator protein DnaA (dnaA) CSB-YP736425SMZ
CSB-EP736425SMZ
CSB-BP736425SMZ
CSB-MP736425SMZ
CSB-EP736425SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable beta-1,3-galactosyltransferase 9 (B3GALT9), partial CSB-YP736426DOA1
CSB-EP736426DOA1
CSB-BP736426DOA1
CSB-MP736426DOA1
CSB-EP736426DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable sugar phosphate/phosphate translocator At3g11320 (At3g11320), partial CSB-YP736427DOA
CSB-EP736427DOA
CSB-BP736427DOA
CSB-MP736427DOA
CSB-EP736427DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Elongation factor Ts, mitochondrial (EFTS) CSB-YP736428DOA
CSB-EP736428DOA
CSB-BP736428DOA
CSB-MP736428DOA
CSB-EP736428DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Protein SEC13 homolog (Sec13) CSB-YP736429RA
CSB-EP736429RA
CSB-BP736429RA
CSB-MP736429RA
CSB-EP736429RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Elongator complex protein 4 (elp4) CSB-YP736430XBE
CSB-EP736430XBE
CSB-BP736430XBE
CSB-MP736430XBE
CSB-EP736430XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis 14-3-3 protein beta/alpha (ywhab) CSB-YP736431XBF
CSB-EP736431XBF
CSB-BP736431XBF
CSB-MP736431XBF
CSB-EP736431XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis F-box/LRR-repeat protein 5 (fbxl5), partial CSB-YP736432XBF
CSB-EP736432XBF
CSB-BP736432XBF
CSB-MP736432XBF
CSB-EP736432XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Src kinase-associated phosphoprotein 2-A (skap2-a) CSB-YP736433XBE
CSB-EP736433XBE
CSB-BP736433XBE
CSB-MP736433XBE
CSB-EP736433XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis DnaJ homolog subfamily B member 6-B (dnajb6-b) CSB-YP736434XBE
CSB-EP736434XBE
CSB-BP736434XBE
CSB-MP736434XBE
CSB-EP736434XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Meiotic nuclear division protein 1 homolog (mnd1) CSB-YP736435XBE
CSB-EP736435XBE
CSB-BP736435XBE
CSB-MP736435XBE
CSB-EP736435XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis V-type proton ATPase subunit C 1 (atp6v1c1) CSB-YP736436XBE
CSB-EP736436XBE
CSB-BP736436XBE
CSB-MP736436XBE
CSB-EP736436XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Proline-rich nuclear receptor coactivator 2 B (pnrc2-b) CSB-YP736437XBE
CSB-EP736437XBE
CSB-BP736437XBE
CSB-MP736437XBE
CSB-EP736437XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Enhancer of mRNA-decapping protein 3 (edc3), partial CSB-YP736438XBE
CSB-EP736438XBE
CSB-BP736438XBE
CSB-MP736438XBE
CSB-EP736438XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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