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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Debaryomyces hansenii Spindle assembly checkpoint component MAD1 (MAD1), partial CSB-YP738233DIS
CSB-EP738233DIS
CSB-BP738233DIS
CSB-MP738233DIS
CSB-EP738233DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Mitochondrial import inner membrane translocase subunit TIM22 (TIM22), partial CSB-YP738234DIS1
CSB-EP738234DIS1
CSB-BP738234DIS1
CSB-MP738234DIS1
CSB-EP738234DIS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii 60S ribosomal protein L7 (RPL7) CSB-YP738235DIS
CSB-EP738235DIS
CSB-BP738235DIS
CSB-MP738235DIS
CSB-EP738235DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii GrpE protein homolog, mitochondrial (mge1) CSB-YP738236DIS
CSB-EP738236DIS
CSB-BP738236DIS
CSB-MP738236DIS
CSB-EP738236DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Vacuolar protein 8 (VAC8), partial CSB-YP738237DIS
CSB-EP738237DIS
CSB-BP738237DIS
CSB-MP738237DIS
CSB-EP738237DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii ATP-dependent RNA helicase DED1 (DED1), partial CSB-YP738238DIS
CSB-EP738238DIS
CSB-BP738238DIS
CSB-MP738238DIS
CSB-EP738238DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Nuclear distribution protein PAC1 (PAC1), partial CSB-YP738239DIS
CSB-EP738239DIS
CSB-BP738239DIS
CSB-MP738239DIS
CSB-EP738239DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Required for respiratory growth protein 9, mitochondrial (RRG9) CSB-YP738240DIS
CSB-EP738240DIS
CSB-BP738240DIS
CSB-MP738240DIS
CSB-EP738240DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Mitochondrial distribution and morphology protein 34 (MDM34), partial CSB-YP738241DIS
CSB-EP738241DIS
CSB-BP738241DIS
CSB-MP738241DIS
CSB-EP738241DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Glucose N-acetyltransferase 1 (GNT1), partial CSB-YP738242DIS
CSB-EP738242DIS
CSB-BP738242DIS
CSB-MP738242DIS
CSB-EP738242DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Pre-mRNA-processing protein 45 (PRP45) CSB-YP738243DIS
CSB-EP738243DIS
CSB-BP738243DIS
CSB-MP738243DIS
CSB-EP738243DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii High osmolarity signaling protein SHO1 (SHO1), partial CSB-YP738244DIS1
CSB-EP738244DIS1
CSB-BP738244DIS1
CSB-MP738244DIS1
CSB-EP738244DIS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Mitochondrial import inner membrane translocase subunit TIM21 (TIM21), partial CSB-YP738245DIS
CSB-EP738245DIS
CSB-BP738245DIS
CSB-MP738245DIS
CSB-EP738245DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Leukotriene A-4 hydrolase homolog (DEHA2B14960g), partial CSB-YP738246DIS
CSB-EP738246DIS
CSB-BP738246DIS
CSB-MP738246DIS
CSB-EP738246DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Pre-mRNA-splicing factor CWC21 (CWC21) CSB-YP738247DIS
CSB-EP738247DIS
CSB-BP738247DIS
CSB-MP738247DIS
CSB-EP738247DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii tRNA (guanine (9)-N1)-methyltransferase (TRM10) CSB-YP738248DIS
CSB-EP738248DIS
CSB-BP738248DIS
CSB-MP738248DIS
CSB-EP738248DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Tethering factor for nuclear proteasome STS1 (STS1) CSB-YP738249DIS
CSB-EP738249DIS
CSB-BP738249DIS
CSB-MP738249DIS
CSB-EP738249DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii E3 ubiquitin-protein ligase BRE1 (BRE1), partial CSB-YP738250DIS
CSB-EP738250DIS
CSB-BP738250DIS
CSB-MP738250DIS
CSB-EP738250DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Probable kinetochore protein SPC24 (SPC24) CSB-YP738251DIS
CSB-EP738251DIS
CSB-BP738251DIS
CSB-MP738251DIS
CSB-EP738251DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii T-complex protein 1 subunit delta (CCT4), partial CSB-YP738252DIS
CSB-EP738252DIS
CSB-BP738252DIS
CSB-MP738252DIS
CSB-EP738252DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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