Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Erwinia carotovora subsp. atroseptica UPF0442 protein ECA3865 (ECA3865), partial CSB-YP738433EAAB1
CSB-EP738433EAAB1
CSB-BP738433EAAB1
CSB-MP738433EAAB1
CSB-EP738433EAAB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Thiamine import ATP-binding protein ThiQ (thiQ) CSB-YP738434EAAB
CSB-EP738434EAAB
CSB-BP738434EAAB
CSB-MP738434EAAB
CSB-EP738434EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) CSB-YP738435EAAB
CSB-EP738435EAAB
CSB-BP738435EAAB
CSB-MP738435EAAB
CSB-EP738435EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica UPF0289 protein ECA3803 (ECA3803) CSB-YP738436EAAB
CSB-EP738436EAAB
CSB-BP738436EAAB
CSB-MP738436EAAB
CSB-EP738436EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica 23S rRNA (guanosine-2'-O-)-methyltransferase RlmB (rlmB) CSB-YP738437EAAB
CSB-EP738437EAAB
CSB-BP738437EAAB
CSB-MP738437EAAB
CSB-EP738437EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Undecaprenyl-diphosphatase (uppP), partial CSB-YP738438EAAB1
CSB-EP738438EAAB1
CSB-BP738438EAAB1
CSB-MP738438EAAB1
CSB-EP738438EAAB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Sulfite reductase [NADPH] flavoprotein alpha-component (cysJ), partial CSB-YP738439EAAB
CSB-EP738439EAAB
CSB-BP738439EAAB
CSB-MP738439EAAB
CSB-EP738439EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica tRNA pseudouridine synthase D (truD) CSB-YP738440EAAB
CSB-EP738440EAAB
CSB-BP738440EAAB
CSB-MP738440EAAB
CSB-EP738440EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Phosphoheptose isomerase (gmhA) CSB-YP738441EAAB
CSB-EP738441EAAB
CSB-BP738441EAAB
CSB-MP738441EAAB
CSB-EP738441EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Regulatory protein recX (recX) CSB-YP738442EAAB
CSB-EP738442EAAB
CSB-BP738442EAAB
CSB-MP738442EAAB
CSB-EP738442EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica 50S ribosomal protein L19 (rplS) CSB-YP738443EAAB
CSB-EP738443EAAB
CSB-BP738443EAAB
CSB-MP738443EAAB
CSB-EP738443EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP738444EAAB
CSB-EP738444EAAB
CSB-BP738444EAAB
CSB-MP738444EAAB
CSB-EP738444EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Glutamate--tRNA ligase (gltX) CSB-YP738445EAAB
CSB-EP738445EAAB
CSB-BP738445EAAB
CSB-MP738445EAAB
CSB-EP738445EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Membrane-bound lytic murein transglycosylase F (mltF) CSB-YP738446EAAB
CSB-EP738446EAAB
CSB-BP738446EAAB
CSB-MP738446EAAB
CSB-EP738446EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Co-chaperone protein hscB (hscB) CSB-YP738447EAAB
CSB-EP738447EAAB
CSB-BP738447EAAB
CSB-MP738447EAAB
CSB-EP738447EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica GTPase Der (der) CSB-YP738448EAAB
CSB-EP738448EAAB
CSB-BP738448EAAB
CSB-MP738448EAAB
CSB-EP738448EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Multidrug resistance protein MdtA (mdtA) CSB-YP738449EAAB
CSB-EP738449EAAB
CSB-BP738449EAAB
CSB-MP738449EAAB
CSB-EP738449EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase (arnT), partial CSB-YP738450EAAB
CSB-EP738450EAAB
CSB-BP738450EAAB
CSB-MP738450EAAB
CSB-EP738450EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Chorismate synthase (aroC) CSB-YP738451EAAB
CSB-EP738451EAAB
CSB-BP738451EAAB
CSB-MP738451EAAB
CSB-EP738451EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica UPF0208 membrane protein ECA3038 (ECA3038), partial CSB-YP738452EAAB1
CSB-EP738452EAAB1
CSB-BP738452EAAB1
CSB-MP738452EAAB1
CSB-EP738452EAAB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>