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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methylococcus capsulatus Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP736780MFM
CSB-EP736780MFM
CSB-BP736780MFM
CSB-MP736780MFM
CSB-EP736780MFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylococcus capsulatus Chaperone protein hscA homolog (hscA), partial CSB-YP736781MFM
CSB-EP736781MFM
CSB-BP736781MFM
CSB-MP736781MFM
CSB-EP736781MFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylococcus capsulatus UPF0102 protein MCA0184 (MCA0184) CSB-YP736782MFM
CSB-EP736782MFM
CSB-BP736782MFM
CSB-MP736782MFM
CSB-EP736782MFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylococcus capsulatus Glutamyl-tRNA (Gln) amidotransferase subunit A (gatA) CSB-YP736783MFM
CSB-EP736783MFM
CSB-BP736783MFM
CSB-MP736783MFM
CSB-EP736783MFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylococcus capsulatus ATP synthase subunit beta (atpD) CSB-YP736784MFM
CSB-EP736784MFM
CSB-BP736784MFM
CSB-MP736784MFM
CSB-EP736784MFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylococcus capsulatus Endonuclease V (nfi) CSB-YP736785MFM
CSB-EP736785MFM
CSB-BP736785MFM
CSB-MP736785MFM
CSB-EP736785MFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum demissum CASP-like protein SDM1_55t00014 (SDM1_55t00014), partial CSB-YP736786SZW1
CSB-EP736786SZW1
CSB-BP736786SZW1
CSB-MP736786SZW1
CSB-EP736786SZW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Secretory carrier-associated membrane protein 3 (SCAMP3), partial CSB-YP736787OFG1
CSB-EP736787OFG1
CSB-BP736787OFG1
CSB-MP736787OFG1
CSB-EP736787OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog 5-hydroxytryptamine receptor 2C (HTR2C), partial CSB-YP736788DO1
CSB-EP736788DO1
CSB-BP736788DO1
CSB-MP736788DO1
CSB-EP736788DO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca fascicularis Gap junction beta-1 protein (GJB1), partial CSB-YP736789MOV1
CSB-EP736789MOV1
CSB-BP736789MOV1
CSB-MP736789MOV1
CSB-EP736789MOV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca fascicularis Tripeptidyl-peptidase 1 (TPP1) CSB-YP736790MOV
CSB-EP736790MOV
CSB-BP736790MOV
CSB-MP736790MOV
CSB-EP736790MOV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca fascicularis Arylsulfatase E (ARSE), partial CSB-YP736791MOV
CSB-EP736791MOV
CSB-BP736791MOV
CSB-MP736791MOV
CSB-EP736791MOV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Interleukin-17A (IL17A) CSB-YP736792PI
CSB-BP736792PI
CSB-MP736792PI
CSB-EP736792PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nucleoporin ndc-1 (npp-22), partial CSB-YP736793CXX1
CSB-EP736793CXX1
CSB-BP736793CXX1
CSB-MP736793CXX1
CSB-EP736793CXX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fidgetin-like protein 1 (figl-1), partial CSB-YP736794CXX
CSB-EP736794CXX
CSB-BP736794CXX
CSB-MP736794CXX
CSB-EP736794CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coiled-coil domain-containing protein 72 homolog (CBG21705) CSB-YP736795CXX
CSB-EP736795CXX
CSB-BP736795CXX
CSB-MP736795CXX
CSB-EP736795CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative steroid dehydrogenase let-767 (let-767) CSB-YP736796CXX
CSB-EP736796CXX
CSB-BP736796CXX
CSB-MP736796CXX
CSB-EP736796CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mediator of RNA polymerase II transcription subunit 6 (mdt-6) CSB-YP736797CXX
CSB-EP736797CXX
CSB-BP736797CXX
CSB-MP736797CXX
CSB-EP736797CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative H/ACA ribonucleoprotein complex subunit 4 (CBG18316) CSB-YP736798CXX
CSB-EP736798CXX
CSB-BP736798CXX
CSB-MP736798CXX
CSB-EP736798CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cell death protein 4 (ced-4), partial CSB-YP736799CXX
CSB-EP736799CXX
CSB-BP736799CXX
CSB-MP736799CXX
CSB-EP736799CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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