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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Mannheimia succiniciproducens RNA pyrophosphohydrolase (rppH) CSB-YP737592MAAB
CSB-EP737592MAAB
CSB-BP737592MAAB
CSB-MP737592MAAB
CSB-EP737592MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Ribonuclease 3 (rnc) CSB-YP737593MAAB
CSB-EP737593MAAB
CSB-BP737593MAAB
CSB-MP737593MAAB
CSB-EP737593MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Na (+)-translocating NADH-quinone reductase subunit A (nqrA) CSB-YP737594MAAB
CSB-EP737594MAAB
CSB-BP737594MAAB
CSB-MP737594MAAB
CSB-EP737594MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Porphobilinogen deaminase (hemC) CSB-YP737595MAAB
CSB-EP737595MAAB
CSB-BP737595MAAB
CSB-MP737595MAAB
CSB-EP737595MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Orotate phosphoribosyltransferase (pyrE) CSB-YP737596MAAB
CSB-EP737596MAAB
CSB-BP737596MAAB
CSB-MP737596MAAB
CSB-EP737596MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP737597MAAB
CSB-EP737597MAAB
CSB-BP737597MAAB
CSB-MP737597MAAB
CSB-EP737597MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens UPF0059 membrane protein MS0192 (MS0192), partial CSB-YP737598MAAB1
CSB-EP737598MAAB1
CSB-BP737598MAAB1
CSB-MP737598MAAB1
CSB-EP737598MAAB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) CSB-YP737599MAAB
CSB-EP737599MAAB
CSB-BP737599MAAB
CSB-MP737599MAAB
CSB-EP737599MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Probable oxaloacetate decarboxylase gamma chain (oadG), partial CSB-YP737600MAAB1
CSB-EP737600MAAB1
CSB-BP737600MAAB1
CSB-MP737600MAAB1
CSB-EP737600MAAB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica FACT complex subunit SSRP1-B (SSRP1-B), partial CSB-YP737601OFG
CSB-EP737601OFG
CSB-BP737601OFG
CSB-MP737601OFG
CSB-EP737601OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica E3 ubiquitin-protein ligase XB3 (XB3) CSB-YP737602OFG
CSB-EP737602OFG
CSB-BP737602OFG
CSB-MP737602OFG
CSB-EP737602OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryphonectria parasitica mycoreovirus 1 Uncharacterized protein VP11 (S11), partial CSB-YP737603CFAT1
CSB-EP737603CFAT1
CSB-BP737603CFAT1
CSB-MP737603CFAT1
CSB-EP737603CFAT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryzias latipes Protein yippee-like 3 (ypel3) CSB-YP737604OFH
CSB-EP737604OFH
CSB-BP737604OFH
CSB-MP737604OFH
CSB-EP737604OFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorocebus aethiops Protein yippee-like 3 (YPEL3) CSB-YP737605DSU
CSB-EP737605DSU
CSB-BP737605DSU
CSB-MP737605DSU
CSB-EP737605DSU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Uncharacterized protein C19D5.10c (SPAC19D5.10c), partial CSB-YP737606SXV1
CSB-EP737606SXV1
CSB-BP737606SXV1
CSB-MP737606SXV1
CSB-EP737606SXV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ornithine carbamoyltransferase, catabolic (arcB) CSB-YP737607BUI
CSB-EP737607BUI
CSB-BP737607BUI
CSB-MP737607BUI
CSB-EP737607BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP737608BUI
CSB-EP737608BUI
CSB-BP737608BUI
CSB-MP737608BUI
CSB-EP737608BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptidyl-tRNA hydrolase (pth) CSB-YP737609BUI
CSB-EP737609BUI
CSB-BP737609BUI
CSB-MP737609BUI
CSB-EP737609BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cocksfoot mottle virus Capsid protein (ORF3) CSB-YP737610CBAJ
CSB-EP737610CBAJ
CSB-BP737610CBAJ
CSB-MP737610CBAJ
CSB-EP737610CBAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60 kDa chaperonin (groL), partial CSB-YP737611BUI
CSB-EP737611BUI
CSB-BP737611BUI
CSB-MP737611BUI
CSB-EP737611BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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