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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mouse Protein FAM179B (Fam179b), partial CSB-YP737993MO
CSB-EP737993MO
CSB-BP737993MO
CSB-MP737993MO
CSB-EP737993MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vulpes lagopus Melanocyte-stimulating hormone receptor (MC1R), partial CSB-YP737994VFU1
CSB-EP737994VFU1
CSB-BP737994VFU1
CSB-MP737994VFU1
CSB-EP737994VFU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Succinate-semialdehyde dehydrogenase, mitochondrial (ALDH5A1) CSB-YP737995EQV
CSB-EP737995EQV
CSB-BP737995EQV
CSB-MP737995EQV
CSB-EP737995EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata Urease (ure1), partial CSB-YP737996NGS
CSB-EP737996NGS
CSB-BP737996NGS
CSB-MP737996NGS
CSB-EP737996NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Putative membrane protein insertion efficiency factor (PPA2351) CSB-YP737997PSG
CSB-EP737997PSG
CSB-BP737997PSG
CSB-MP737997PSG
CSB-EP737997PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Undecaprenyl-diphosphatase (uppP), partial CSB-YP737998PSG
CSB-EP737998PSG
CSB-BP737998PSG
CSB-MP737998PSG
CSB-EP737998PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes 50S ribosomal protein L33 2 (rpmG2) CSB-YP737999PSG
CSB-EP737999PSG
CSB-BP737999PSG
CSB-MP737999PSG
CSB-EP737999PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Uncharacterized transporter PPA2034 (PPA2034), partial CSB-YP738000PSG
CSB-EP738000PSG
CSB-BP738000PSG
CSB-MP738000PSG
CSB-EP738000PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP738001PSG
CSB-EP738001PSG
CSB-BP738001PSG
CSB-MP738001PSG
CSB-EP738001PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes 50S ribosomal protein L1 (rplA) CSB-YP738002PSG
CSB-EP738002PSG
CSB-BP738002PSG
CSB-MP738002PSG
CSB-EP738002PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Biotin biosynthesis bifunctional protein BioWF (PPA1866), partial CSB-YP738003PSG
CSB-EP738003PSG
CSB-BP738003PSG
CSB-MP738003PSG
CSB-EP738003PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes 50S ribosomal protein L22 (rplV) CSB-YP738004PSG
CSB-EP738004PSG
CSB-BP738004PSG
CSB-MP738004PSG
CSB-EP738004PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes 30S ribosomal protein S8 (rpsH) CSB-YP738005PSG
CSB-EP738005PSG
CSB-BP738005PSG
CSB-MP738005PSG
CSB-EP738005PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes 50S ribosomal protein L36 (rpmJ) CSB-YP738006PSG
CSB-EP738006PSG
CSB-BP738006PSG
CSB-MP738006PSG
CSB-EP738006PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes tRNA pseudouridine synthase A (truA) CSB-YP738007PSG
CSB-EP738007PSG
CSB-BP738007PSG
CSB-MP738007PSG
CSB-EP738007PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP738008PSG
CSB-EP738008PSG
CSB-BP738008PSG
CSB-MP738008PSG
CSB-EP738008PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Maf-like protein PPA1709 (PPA1709) CSB-YP738009PSG
CSB-EP738009PSG
CSB-BP738009PSG
CSB-MP738009PSG
CSB-EP738009PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Trigger factor (tig), partial CSB-YP738010PSG
CSB-EP738010PSG
CSB-BP738010PSG
CSB-MP738010PSG
CSB-EP738010PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Elongation factor Ts (tsf) CSB-YP738011PSG
CSB-EP738011PSG
CSB-BP738011PSG
CSB-MP738011PSG
CSB-EP738011PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Translation initiation factor IF-2 (infB), partial CSB-YP738012PSG
CSB-EP738012PSG
CSB-BP738012PSG
CSB-MP738012PSG
CSB-EP738012PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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