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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Candida glabrata Autophagy protein 16 (ATG16) CSB-YP739015CZI
CSB-EP739015CZI
CSB-BP739015CZI
CSB-MP739015CZI
CSB-EP739015CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Palmitoyltransferase AKR1 (AKR1), partial CSB-YP739016CZI1
CSB-EP739016CZI1
CSB-BP739016CZI1
CSB-MP739016CZI1
CSB-EP739016CZI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Pyruvate decarboxylase (PDC1), partial CSB-YP739017CZI
CSB-EP739017CZI
CSB-BP739017CZI
CSB-MP739017CZI
CSB-EP739017CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Mitochondrial inner membrane magnesium transporter LPE10 (LPE10), partial CSB-YP739018CZI1
CSB-EP739018CZI1
CSB-BP739018CZI1
CSB-MP739018CZI1
CSB-EP739018CZI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Nuclear protein localization protein 4 (NPL4), partial CSB-YP739019CZI
CSB-EP739019CZI
CSB-BP739019CZI
CSB-MP739019CZI
CSB-EP739019CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Alpha-1,3/1,6-mannosyltransferase ALG2 (ALG2), partial CSB-YP739020CZI1
CSB-EP739020CZI1
CSB-BP739020CZI1
CSB-MP739020CZI1
CSB-EP739020CZI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Defective in cullin neddylation protein 1 (DCN1) CSB-YP739021CZI
CSB-EP739021CZI
CSB-BP739021CZI
CSB-MP739021CZI
CSB-EP739021CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Histone H1 (HHOA) CSB-YP739022CZI
CSB-EP739022CZI
CSB-BP739022CZI
CSB-MP739022CZI
CSB-EP739022CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Sensitive to high expression protein 9 homolog, mitochondrial (SHE9), partial CSB-YP739023CZI
CSB-EP739023CZI
CSB-BP739023CZI
CSB-MP739023CZI
CSB-EP739023CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata 40S ribosomal protein S1 (RPS1) CSB-YP739024CZI
CSB-EP739024CZI
CSB-BP739024CZI
CSB-MP739024CZI
CSB-EP739024CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Mitochondrial genome maintenance protein MGM101 (MGM101) CSB-YP739025CZI
CSB-EP739025CZI
CSB-BP739025CZI
CSB-MP739025CZI
CSB-EP739025CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Translationally-controlled tumor protein homolog (CAGL0L12870g) CSB-YP739026CZI
CSB-EP739026CZI
CSB-BP739026CZI
CSB-MP739026CZI
CSB-EP739026CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata pH-response regulator protein palA/RIM20 (RIM20), partial CSB-YP739027CZI
CSB-EP739027CZI
CSB-BP739027CZI
CSB-MP739027CZI
CSB-EP739027CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata 5'-3' exoribonuclease 2 (RAT1), partial CSB-YP739028CZI
CSB-EP739028CZI
CSB-BP739028CZI
CSB-MP739028CZI
CSB-EP739028CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Dimethyladenosine transferase (DIM1) CSB-YP739029CZI
CSB-EP739029CZI
CSB-BP739029CZI
CSB-MP739029CZI
CSB-EP739029CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata KNR4/SMI1 homolog (CAGL0L06534g), partial CSB-YP739030CZI
CSB-EP739030CZI
CSB-BP739030CZI
CSB-MP739030CZI
CSB-EP739030CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Ribosome biogenesis protein ALB1 (ALB1) CSB-YP739031CZI
CSB-EP739031CZI
CSB-BP739031CZI
CSB-MP739031CZI
CSB-EP739031CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Protein PNS1 (PNS1), partial CSB-YP739032CZI
CSB-EP739032CZI
CSB-BP739032CZI
CSB-MP739032CZI
CSB-EP739032CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Cytoplasmic tRNA 2-thiolation protein 2 (NCS2) CSB-YP739033CZI
CSB-EP739033CZI
CSB-BP739033CZI
CSB-MP739033CZI
CSB-EP739033CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata CCR4-associated factor 4 homolog (CAF4), partial CSB-YP739034CZI
CSB-EP739034CZI
CSB-BP739034CZI
CSB-MP739034CZI
CSB-EP739034CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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