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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Acinetobacter sp. Phosphoglycerate kinase (pgk) CSB-YP738855AWW
CSB-EP738855AWW
CSB-BP738855AWW
CSB-MP738855AWW
CSB-EP738855AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. GTP cyclohydrolase folE2 (folE2) CSB-YP738856AWW
CSB-EP738856AWW
CSB-BP738856AWW
CSB-MP738856AWW
CSB-EP738856AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Chaperone protein hscA homolog (hscA), partial CSB-YP738857AWW
CSB-EP738857AWW
CSB-BP738857AWW
CSB-MP738857AWW
CSB-EP738857AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Regulatory protein recX (recX) CSB-YP738858AWW
CSB-EP738858AWW
CSB-BP738858AWW
CSB-MP738858AWW
CSB-EP738858AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP738859AWW
CSB-EP738859AWW
CSB-BP738859AWW
CSB-MP738859AWW
CSB-EP738859AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Ribose-5-phosphate isomerase A (rpiA) CSB-YP738860AWW
CSB-EP738860AWW
CSB-BP738860AWW
CSB-MP738860AWW
CSB-EP738860AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. N-succinylarginine dihydrolase (astB) CSB-YP738861AWW
CSB-EP738861AWW
CSB-BP738861AWW
CSB-MP738861AWW
CSB-EP738861AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP738862AWW
CSB-EP738862AWW
CSB-BP738862AWW
CSB-MP738862AWW
CSB-EP738862AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP738863AWW
CSB-EP738863AWW
CSB-BP738863AWW
CSB-MP738863AWW
CSB-EP738863AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Urease accessory protein UreE (ureE) CSB-YP738864AWW
CSB-EP738864AWW
CSB-BP738864AWW
CSB-MP738864AWW
CSB-EP738864AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Betaine aldehyde dehydrogenase (betB) CSB-YP738865AWW
CSB-EP738865AWW
CSB-BP738865AWW
CSB-MP738865AWW
CSB-EP738865AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Undecaprenyl-diphosphatase 2 (uppP2), partial CSB-YP738866AWW
CSB-EP738866AWW
CSB-BP738866AWW
CSB-MP738866AWW
CSB-EP738866AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 30S ribosomal protein S7 (rpsG) CSB-YP738867AWW
CSB-EP738867AWW
CSB-BP738867AWW
CSB-MP738867AWW
CSB-EP738867AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Deoxycytidine triphosphate deaminase (dcd) CSB-YP738868AWW
CSB-EP738868AWW
CSB-BP738868AWW
CSB-MP738868AWW
CSB-EP738868AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP738869AWW
CSB-EP738869AWW
CSB-BP738869AWW
CSB-MP738869AWW
CSB-EP738869AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Ribosomal RNA small subunit methyltransferase C (rsmC) CSB-YP738870AWW
CSB-EP738870AWW
CSB-BP738870AWW
CSB-MP738870AWW
CSB-EP738870AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP738871AWW
CSB-EP738871AWW
CSB-BP738871AWW
CSB-MP738871AWW
CSB-EP738871AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Aspartate--tRNA ligase (aspS), partial CSB-YP738872AWW
CSB-EP738872AWW
CSB-BP738872AWW
CSB-MP738872AWW
CSB-EP738872AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Non-canonical purine NTP pyrophosphatase (ACIAD0526) CSB-YP738873AWW
CSB-EP738873AWW
CSB-BP738873AWW
CSB-MP738873AWW
CSB-EP738873AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 50S ribosomal protein L28 (rpmB) CSB-YP738874AWW
CSB-EP738874AWW
CSB-BP738874AWW
CSB-MP738874AWW
CSB-EP738874AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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