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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Methanococcus maripaludis Translation initiation factor 5A (eif5a) CSB-YP740455MSB
CSB-EP740455MSB
CSB-BP740455MSB
CSB-MP740455MSB
CSB-EP740455MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis F420-0:gamma-glutamyl ligase (cofE) CSB-YP740456MSB
CSB-EP740456MSB
CSB-BP740456MSB
CSB-MP740456MSB
CSB-EP740456MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0333 protein MMP0903 (MMP0903), partial CSB-YP740457MSB1
CSB-EP740457MSB1
CSB-BP740457MSB1
CSB-MP740457MSB1
CSB-EP740457MSB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UvrABC system protein C (uvrC), partial CSB-YP740458MSB
CSB-EP740458MSB
CSB-BP740458MSB
CSB-MP740458MSB
CSB-EP740458MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Uracil phosphoribosyltransferase (upp) CSB-YP740459MSB
CSB-EP740459MSB
CSB-BP740459MSB
CSB-MP740459MSB
CSB-EP740459MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0237 protein MMP0657 (MMP0657) CSB-YP740460MSB
CSB-EP740460MSB
CSB-BP740460MSB
CSB-MP740460MSB
CSB-EP740460MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S17e (rps17e) CSB-YP740461MSB
CSB-EP740461MSB
CSB-BP740461MSB
CSB-MP740461MSB
CSB-EP740461MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Threonine--tRNA ligase (thrS), partial CSB-YP740462MSB
CSB-EP740462MSB
CSB-BP740462MSB
CSB-MP740462MSB
CSB-EP740462MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Uridylate kinase (pyrH) CSB-YP740463MSB
CSB-EP740463MSB
CSB-BP740463MSB
CSB-MP740463MSB
CSB-EP740463MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Replication factor C large subunit (rfcL) CSB-YP740464MSB
CSB-EP740464MSB
CSB-BP740464MSB
CSB-MP740464MSB
CSB-EP740464MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP740465MSB
CSB-EP740465MSB
CSB-BP740465MSB
CSB-MP740465MSB
CSB-EP740465MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis L-tyrosine decarboxylase (mfnA) CSB-YP740466MSB
CSB-EP740466MSB
CSB-BP740466MSB
CSB-MP740466MSB
CSB-EP740466MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis PyrE-like protein (MMP0079) CSB-YP740467MSB
CSB-EP740467MSB
CSB-BP740467MSB
CSB-MP740467MSB
CSB-EP740467MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein LX (rplX) CSB-YP740468MSB
CSB-EP740468MSB
CSB-BP740468MSB
CSB-MP740468MSB
CSB-EP740468MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 3-dehydroquinate synthase (MMP0006) CSB-YP740469MSB
CSB-EP740469MSB
CSB-BP740469MSB
CSB-MP740469MSB
CSB-EP740469MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Error-prone DNA polymerase (dnaE2), partial CSB-YP740470DWY
CSB-EP740470DWY
CSB-BP740470DWY
CSB-MP740470DWY
CSB-EP740470DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Uridylate kinase (pyrH) CSB-YP740471PAAD
CSB-EP740471PAAD
CSB-BP740471PAAD
CSB-MP740471PAAD
CSB-EP740471PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP740472PAAD
CSB-EP740472PAAD
CSB-BP740472PAAD
CSB-MP740472PAAD
CSB-EP740472PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP740473PAAD
CSB-EP740473PAAD
CSB-BP740473PAAD
CSB-MP740473PAAD
CSB-EP740473PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila ATP synthase subunit delta (atpH) CSB-YP740474PAAD
CSB-EP740474PAAD
CSB-BP740474PAAD
CSB-MP740474PAAD
CSB-EP740474PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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