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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Candida glabrata mRNA-capping enzyme subunit alpha (CEG1) CSB-YP738795CZI
CSB-EP738795CZI
CSB-BP738795CZI
CSB-MP738795CZI
CSB-EP738795CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Mediator of RNA polymerase II transcription subunit 10 (NUT2) CSB-YP738796CZI
CSB-EP738796CZI
CSB-BP738796CZI
CSB-MP738796CZI
CSB-EP738796CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata tRNA (guanine (9)-N1)-methyltransferase (TRM10) CSB-YP738797CZI
CSB-EP738797CZI
CSB-BP738797CZI
CSB-MP738797CZI
CSB-EP738797CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Protein BSP1 (BSP1), partial CSB-YP738798CZI
CSB-EP738798CZI
CSB-BP738798CZI
CSB-MP738798CZI
CSB-EP738798CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata J protein JJJ2 (JJJ2) CSB-YP738799CZI
CSB-EP738799CZI
CSB-BP738799CZI
CSB-MP738799CZI
CSB-EP738799CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Ubiquitin-conjugating enzyme E2 6 (UBC6), partial CSB-YP738800CZI
CSB-EP738800CZI
CSB-BP738800CZI
CSB-MP738800CZI
CSB-EP738800CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Ubiquitin-like modifier-activating enzyme ATG7 (ATG7), partial CSB-YP738801CZI
CSB-EP738801CZI
CSB-BP738801CZI
CSB-MP738801CZI
CSB-EP738801CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Serine/threonine-protein phosphatase 4 regulatory subunit 3 (PSY2), partial CSB-YP738802CZI
CSB-EP738802CZI
CSB-BP738802CZI
CSB-MP738802CZI
CSB-EP738802CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata 40S ribosomal protein S16 (RPS16) CSB-YP738803CZI
CSB-EP738803CZI
CSB-BP738803CZI
CSB-MP738803CZI
CSB-EP738803CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Autophagy-related protein 20 (ATG20), partial CSB-YP738804CZI
CSB-EP738804CZI
CSB-BP738804CZI
CSB-MP738804CZI
CSB-EP738804CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata 60S ribosomal protein L43 (RPL43) CSB-YP738805CZI
CSB-EP738805CZI
CSB-BP738805CZI
CSB-MP738805CZI
CSB-EP738805CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Peptide-N (4)- (N-acetyl-beta-glucosaminyl)asparagine amidase (PNG1) CSB-YP738806CZI
CSB-EP738806CZI
CSB-BP738806CZI
CSB-MP738806CZI
CSB-EP738806CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata COPII coat assembly protein SEC16 (SEC16), partial CSB-YP738807CZI
CSB-EP738807CZI
CSB-BP738807CZI
CSB-MP738807CZI
CSB-EP738807CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesoplasma florum Uridylate kinase (pyrH) CSB-YP738808MEO
CSB-EP738808MEO
CSB-BP738808MEO
CSB-MP738808MEO
CSB-EP738808MEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesoplasma florum Arginine--tRNA ligase (argS), partial CSB-YP738809MEO
CSB-EP738809MEO
CSB-BP738809MEO
CSB-MP738809MEO
CSB-EP738809MEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesoplasma florum NH (3)-dependent NAD (+) synthetase (nadE) CSB-YP738810MEO
CSB-EP738810MEO
CSB-BP738810MEO
CSB-MP738810MEO
CSB-EP738810MEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesoplasma florum 50S ribosomal protein L21 (rplU) CSB-YP738811MEO
CSB-EP738811MEO
CSB-BP738811MEO
CSB-MP738811MEO
CSB-EP738811MEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesoplasma florum tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP738812MEO
CSB-EP738812MEO
CSB-BP738812MEO
CSB-MP738812MEO
CSB-EP738812MEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesoplasma florum Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP738813MEO1
CSB-EP738813MEO1
CSB-BP738813MEO1
CSB-MP738813MEO1
CSB-EP738813MEO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesoplasma florum tRNA pseudouridine synthase B (truB) CSB-YP738814MEO
CSB-EP738814MEO
CSB-BP738814MEO
CSB-MP738814MEO
CSB-EP738814MEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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