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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rabbit Protein S100-B (S100B) CSB-YP740995RB
CSB-EP740995RB
CSB-BP740995RB
CSB-MP740995RB
CSB-EP740995RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Kynurenine--oxoglutarate transaminase 3 (CCBL2) CSB-YP740996HU
CSB-BP740996HU
CSB-MP740996HU
CSB-EP740996HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma 50S ribosomal protein L20 (rplT) CSB-YP740997OBN
CSB-EP740997OBN
CSB-BP740997OBN
CSB-MP740997OBN
CSB-EP740997OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Heat-inducible transcription repressor HrcA (hrcA) CSB-YP740998OBN
CSB-EP740998OBN
CSB-BP740998OBN
CSB-MP740998OBN
CSB-EP740998OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma DNA ligase (ligA), partial CSB-YP740999OBN
CSB-EP740999OBN
CSB-BP740999OBN
CSB-MP740999OBN
CSB-EP740999OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Elongation factor P (efp) CSB-YP741000OBN
CSB-EP741000OBN
CSB-BP741000OBN
CSB-MP741000OBN
CSB-EP741000OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma 50S ribosomal protein L1 (rplA) CSB-YP741001OBN
CSB-EP741001OBN
CSB-BP741001OBN
CSB-MP741001OBN
CSB-EP741001OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma 50S ribosomal protein L17 (rplQ) CSB-YP741002OBN
CSB-EP741002OBN
CSB-BP741002OBN
CSB-MP741002OBN
CSB-EP741002OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma 50S ribosomal protein L30 (rpmD) CSB-YP741003OBN
CSB-EP741003OBN
CSB-BP741003OBN
CSB-MP741003OBN
CSB-EP741003OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma 30S ribosomal protein S17 (rpsQ) CSB-YP741004OBN
CSB-EP741004OBN
CSB-BP741004OBN
CSB-MP741004OBN
CSB-EP741004OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Isoleucine--tRNA ligase (ileS), partial CSB-YP741005OBN
CSB-EP741005OBN
CSB-BP741005OBN
CSB-MP741005OBN
CSB-EP741005OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Triosephosphate isomerase (tpiA) CSB-YP741006OBN
CSB-EP741006OBN
CSB-BP741006OBN
CSB-MP741006OBN
CSB-EP741006OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma SsrA-binding protein (smpB) CSB-YP741007OBN
CSB-EP741007OBN
CSB-BP741007OBN
CSB-MP741007OBN
CSB-EP741007OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma 30S ribosomal protein S9 (rpsI) CSB-YP741008OBN
CSB-EP741008OBN
CSB-BP741008OBN
CSB-MP741008OBN
CSB-EP741008OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma 50S ribosomal protein L31 (rpmE) CSB-YP741009OBN
CSB-EP741009OBN
CSB-BP741009OBN
CSB-MP741009OBN
CSB-EP741009OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Putative ABC transporter ATP-binding protein PAM_020 (PAM_020), partial CSB-YP741010OBN
CSB-EP741010OBN
CSB-BP741010OBN
CSB-MP741010OBN
CSB-EP741010OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma 30S ribosomal protein S18 (rpsR) CSB-YP741011OBN
CSB-EP741011OBN
CSB-BP741011OBN
CSB-MP741011OBN
CSB-EP741011OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica PHD finger protein ALFIN-LIKE 9 (Os07g0608400, LOC_Os07g41740) CSB-YP741012OFG
CSB-EP741012OFG
CSB-BP741012OFG
CSB-MP741012OFG
CSB-EP741012OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Potassium transporter 25 (HAK25), partial CSB-YP741013OFG
CSB-EP741013OFG
CSB-BP741013OFG
CSB-MP741013OFG
CSB-EP741013OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens 30S ribosomal protein S2, chloroplastic (rps2) CSB-YP741014EVD
CSB-EP741014EVD
CSB-BP741014EVD
CSB-MP741014EVD
CSB-EP741014EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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