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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human Progestin and adipoQ receptor family member 9 (PAQR9), partial CSB-YP741075HU1
CSB-EP741075HU1
CSB-BP741075HU1
CSB-MP741075HU1
CSB-EP741075HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human UPF0627 protein ENSP00000358171 CSB-YP741076HU
CSB-EP741076HU
CSB-BP741076HU
CSB-MP741076HU
CSB-EP741076HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Pleckstrin homology domain-containing family M member 3 (PLEKHM3) CSB-YP741077HU
CSB-EP741077HU
CSB-BP741077HU
CSB-MP741077HU
CSB-EP741077HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Nesprin-2 (Syne2), partial CSB-YP741078MO
CSB-EP741078MO
CSB-BP741078MO
CSB-MP741078MO
CSB-EP741078MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Eukaryotic translation initiation factor 2 subunit 1 (Eif2s1) CSB-YP741079MO
CSB-EP741079MO
CSB-BP741079MO
CSB-MP741079MO
CSB-EP741079MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Prothyroliberin (TRH) CSB-YP741080CH
CSB-EP741080CH
CSB-BP741080CH
CSB-MP741080CH
CSB-EP741080CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyberlindnera jadinii Glycerol-3-phosphate dehydrogenase [NAD (+)] 2 (gpd2) CSB-YP741081EPX
CSB-EP741081EPX
CSB-BP741081EPX
CSB-MP741081EPX
CSB-EP741081EPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Zinc finger protein JACKDAW (JKD), partial CSB-YP741082DOA
CSB-EP741082DOA
CSB-BP741082DOA
CSB-MP741082DOA
CSB-EP741082DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat SCO-spondin (Sspo), partial CSB-YP741083RA
CSB-EP741083RA
CSB-BP741083RA
CSB-MP741083RA
CSB-EP741083RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Keratin-associated protein 5-2 (KRTAP5-2) CSB-YP741084HU
CSB-EP741084HU
CSB-BP741084HU
CSB-MP741084HU
CSB-EP741084HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus tenax Malate dehydrogenase (mdh) CSB-YP741085TDN
CSB-EP741085TDN
CSB-BP741085TDN
CSB-MP741085TDN
CSB-EP741085TDN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein E7 (E7) CSB-YP741086HDAE
CSB-EP741086HDAE
CSB-BP741086HDAE
CSB-MP741086HDAE
CSB-EP741086HDAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Minor capsid protein L2 (L2) CSB-YP741087HNP
CSB-EP741087HNP
CSB-BP741087HNP
CSB-MP741087HNP
CSB-EP741087HNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Vpu (vpu), partial CSB-YP741088HKU
CSB-EP741088HKU
CSB-BP741088HKU
CSB-MP741088HKU
CSB-EP741088HKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pichia angusta Mating-type protein ALPHA1 (MATALPHA1) CSB-YP741089EVQ
CSB-EP741089EVQ
CSB-BP741089EVQ
CSB-MP741089EVQ
CSB-EP741089EVQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Mating-type-like protein A1 (MTL1A1) CSB-YP741090CZI
CSB-EP741090CZI
CSB-BP741090CZI
CSB-MP741090CZI
CSB-EP741090CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Forkhead box protein J1-A (foxj1-a) CSB-YP741091XBE
CSB-EP741091XBE
CSB-BP741091XBE
CSB-MP741091XBE
CSB-EP741091XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spalax judaei Cryptochrome-1 (CRY1), partial CSB-YP741092SAAG
CSB-EP741092SAAG
CSB-BP741092SAAG
CSB-MP741092SAAG
CSB-EP741092SAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Enolase (ENO) CSB-YP741093KBK
CSB-EP741093KBK
CSB-BP741093KBK
CSB-MP741093KBK
CSB-EP741093KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mandragora officinarum Maturase K (matK), partial CSB-YP741094MFAE
CSB-EP741094MFAE
CSB-BP741094MFAE
CSB-MP741094MFAE
CSB-EP741094MFAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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