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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Helicobacter hepaticus UPF0102 protein HH_1751 (HH_1751) CSB-YP742797HCO
CSB-EP742797HCO
CSB-BP742797HCO
CSB-MP742797HCO
CSB-EP742797HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Histidinol dehydrogenase (hisD) CSB-YP742798HCO
CSB-EP742798HCO
CSB-BP742798HCO
CSB-MP742798HCO
CSB-EP742798HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Apolipoprotein N-acyltransferase (lnt), partial CSB-YP742799HCO1
CSB-EP742799HCO1
CSB-BP742799HCO1
CSB-MP742799HCO1
CSB-EP742799HCO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP742800HCO
CSB-EP742800HCO
CSB-BP742800HCO
CSB-MP742800HCO
CSB-EP742800HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus S-adenosylmethionine synthase (metK) CSB-YP742801HCO
CSB-EP742801HCO
CSB-BP742801HCO
CSB-MP742801HCO
CSB-EP742801HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus 50S ribosomal protein L25 (rplY) CSB-YP742802HCO
CSB-EP742802HCO
CSB-BP742802HCO
CSB-MP742802HCO
CSB-EP742802HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Flagellar hook-basal body complex protein FliE (fliE) CSB-YP742803HCO
CSB-EP742803HCO
CSB-BP742803HCO
CSB-MP742803HCO
CSB-EP742803HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus 50S ribosomal protein L18 (rplR) CSB-YP742804HCO
CSB-EP742804HCO
CSB-BP742804HCO
CSB-MP742804HCO
CSB-EP742804HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus 50S ribosomal protein L16 (rplP) CSB-YP742805HCO
CSB-EP742805HCO
CSB-BP742805HCO
CSB-MP742805HCO
CSB-EP742805HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus 30S ribosomal protein S10 (rpsJ) CSB-YP742806HCO
CSB-EP742806HCO
CSB-BP742806HCO
CSB-MP742806HCO
CSB-EP742806HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP742807HCO
CSB-EP742807HCO
CSB-BP742807HCO
CSB-MP742807HCO
CSB-EP742807HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP742808HCO
CSB-EP742808HCO
CSB-BP742808HCO
CSB-MP742808HCO
CSB-EP742808HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Argininosuccinate synthase (argG) CSB-YP742809HCO
CSB-EP742809HCO
CSB-BP742809HCO
CSB-MP742809HCO
CSB-EP742809HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP742810HCO
CSB-EP742810HCO
CSB-BP742810HCO
CSB-MP742810HCO
CSB-EP742810HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Elongation factor Ts (tsf) CSB-YP742811HCO
CSB-EP742811HCO
CSB-BP742811HCO
CSB-MP742811HCO
CSB-EP742811HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP742812HCO
CSB-EP742812HCO
CSB-BP742812HCO
CSB-MP742812HCO
CSB-EP742812HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Glutamate--tRNA ligase 1 (gltX1) CSB-YP742813HCO
CSB-EP742813HCO
CSB-BP742813HCO
CSB-MP742813HCO
CSB-EP742813HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Flagellar assembly factor FliW 1 (fliW1) CSB-YP742814HCO
CSB-EP742814HCO
CSB-BP742814HCO
CSB-MP742814HCO
CSB-EP742814HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Probable hydrogenase nickel incorporation protein HypA (hypA) CSB-YP742815HCO
CSB-EP742815HCO
CSB-BP742815HCO
CSB-MP742815HCO
CSB-EP742815HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus L-seryl-tRNA (Sec) selenium transferase (selA) CSB-YP742816HCO
CSB-EP742816HCO
CSB-BP742816HCO
CSB-MP742816HCO
CSB-EP742816HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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