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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Geobacter sulfurreducens Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP741495GBK
CSB-EP741495GBK
CSB-BP741495GBK
CSB-MP741495GBK
CSB-EP741495GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE), partial CSB-YP741496GBK
CSB-EP741496GBK
CSB-BP741496GBK
CSB-MP741496GBK
CSB-EP741496GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Flagellar P-ring protein (flgI) CSB-YP741497GBK
CSB-EP741497GBK
CSB-BP741497GBK
CSB-MP741497GBK
CSB-EP741497GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 3-isopropylmalate dehydrogenase (leuB) CSB-YP741498GBK
CSB-EP741498GBK
CSB-BP741498GBK
CSB-MP741498GBK
CSB-EP741498GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Elongation factor G 2 (fusA2), partial CSB-YP741499GBK
CSB-EP741499GBK
CSB-BP741499GBK
CSB-MP741499GBK
CSB-EP741499GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 50S ribosomal protein L29 (rpmC) CSB-YP741500GBK
CSB-EP741500GBK
CSB-BP741500GBK
CSB-MP741500GBK
CSB-EP741500GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 50S ribosomal protein L30 (rpmD) CSB-YP741501GBK
CSB-EP741501GBK
CSB-BP741501GBK
CSB-MP741501GBK
CSB-EP741501GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Glycerol kinase (glpK) CSB-YP741502GBK
CSB-EP741502GBK
CSB-BP741502GBK
CSB-MP741502GBK
CSB-EP741502GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP741503GBK
CSB-EP741503GBK
CSB-BP741503GBK
CSB-MP741503GBK
CSB-EP741503GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Potassium-transporting ATPase A chain (kdpA), partial CSB-YP741504GBK
CSB-EP741504GBK
CSB-BP741504GBK
CSB-MP741504GBK
CSB-EP741504GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Na (+)/H (+) antiporter nhaA (nhaA), partial CSB-YP741505GBK
CSB-EP741505GBK
CSB-BP741505GBK
CSB-MP741505GBK
CSB-EP741505GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 50S ribosomal protein L28 (rpmB) CSB-YP741506GBK
CSB-EP741506GBK
CSB-BP741506GBK
CSB-MP741506GBK
CSB-EP741506GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP741507GBK
CSB-EP741507GBK
CSB-BP741507GBK
CSB-MP741507GBK
CSB-EP741507GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Shikimate kinase (aroK) CSB-YP741508GBK
CSB-EP741508GBK
CSB-BP741508GBK
CSB-MP741508GBK
CSB-EP741508GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Uridylate kinase (pyrH) CSB-YP741509GBK
CSB-EP741509GBK
CSB-BP741509GBK
CSB-MP741509GBK
CSB-EP741509GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP741510GBK
CSB-EP741510GBK
CSB-BP741510GBK
CSB-MP741510GBK
CSB-EP741510GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Ribonuclease PH (rph) CSB-YP741511GBK
CSB-EP741511GBK
CSB-BP741511GBK
CSB-MP741511GBK
CSB-EP741511GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP741512GBK
CSB-EP741512GBK
CSB-BP741512GBK
CSB-MP741512GBK
CSB-EP741512GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens N utilization substance protein B homolog (nusB) CSB-YP741513GBK
CSB-EP741513GBK
CSB-BP741513GBK
CSB-MP741513GBK
CSB-EP741513GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Ribosome-binding factor A (rbfA) CSB-YP741514GBK
CSB-EP741514GBK
CSB-BP741514GBK
CSB-MP741514GBK
CSB-EP741514GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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